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Role of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa
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Role of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa
Somanath, P.R.; Gandhi, K.K.;
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There are several physiological and pharmacological evidences indicating that opening of voltage dependent channels play a critical role in induction of acrosome reaction in mammalian sperm. We determined the intracellular free concentration in ejaculated goat sperm using a fluorescent, -specific probe, Fura2/AM, after the suspension of sperm in KRB medium, capable of sustaining capacitation and the acrosome reaction. We used nifedipine, D-600 and diltiazem, the channel antagonists belonging to the classes of dihydropyridines, phenylalkylamines and benzothiazepines, to investigate the possibility that L-type voltage gated channels play a role in the progesterone-stimulated exocytotic response. Progesterone promoted a rise in intracellular in goat sperm and addition of nifedipine (100 nM) just prior to progesterone induction, significantly inhibited both intracellular rise and exocytosis suggesting that channels are involved in the process. However, the intracellular increase during the process of capacitation was not affected with the addition of nifedipine suggesting a role of focal channel for during capacitation. Studies using monensin and nigericin, two monovalent cation ionophores showed that an influx of also may play a role in the opening of channels. These results strongly suggests that the entry of channels with characteristics similar to those of L-type, voltage-sensitive channels found in cardiac and skeletal muscle, is a crucial step in the sequence of events leading to progesterone induced acrosome reaction in goat sperm.
Goat Sperm;Nifedipine;Monensin;Nigericin;;Channels;Fura 2;
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