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Influence of the Novel Urease Inhibitor Hydroquinone on Growing Lamb Nitrogen Utilization
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 Title & Authors
Influence of the Novel Urease Inhibitor Hydroquinone on Growing Lamb Nitrogen Utilization
Zhang, Y.G.; Shan, A.S.; Bao, J.;
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 Abstract
Two in vivo experiments were conducted to evaluate the effect of novel urease inhibitor hydroquinone (HQ) on ammonia release rate from urea hydrolysis, nitrogen balance, nutrient digestibility and efficiency of microbial protein synthesis. In Exp. 1, twelve crossbred cannulated lambs were randomly assigned within initial body weight block to one of four HQ treatments, which included 0 (control), 30, 60 or 80 mg HQ/kg DM intake. Ammonia concentration and pH of ruminal fluid were immediately measured at 0, 2, 4, 6 and 8 h after feeding. Increasing the dose of HQ tended (p<0.15) to linearly decrease NH3 formation. The ammonia peak concentration (2 h post-feeding) in animals receiving HQ was approximately one-half of that in animals not receiving HQ (p<0.01), and a relatively sustained ammonia release could be obtained at the dose of 30 or 60 mg HQ/kg DM. In Exp. 2, sixteen intact crossbred lambs (weight ) were used in a factorial design experiment. The four rations consisting of soybean meal-based (SBM) or urea-based (Urea) nitrogen source with or without HQ (S1, S0, U1 and U0) were fed in digestion and N balance trials. Apparent digestibility of major nutrients except that of ADF was not affected by either nitrogen source or addition of HQ. Regardless of nitrogen source, supplementation of HQ significantly improved ADF digestibility (p<0.05). The various ration had no effects on N metabolism in the presence of HQ. There was significant difference between total purine derivatives (PD), estimated efficiency of microbial N synthesis (p<0.05) and urea-N excretion (p<0.01) in the urine for the SBM ration and for the Urea ration. However, HQ had little influence on efficiency of microbial N synthesis as proportion of daily intake of total tract digestible OM (p>0.05). No interactions between main nitrogen source and HQ were measured throughout the trial. Results of this study suggest that addition of HQ to ration may improve ADF digestion with having no negative effect on N metabolism and microbial protein production.
 Keywords
Hydroquinone;Urea;Nitrogen Utilization;Lamb;
 Language
English
 Cited by
 References
1.
AOAC. 1975. Official Methods of Analysis (12th Ed.). Association of Official Analysis Chemists, Washington, DC.

2.
Chen, X. B., F. D. Deb, Hovell, E. R. Orskov and D. S. Brown 1990. Excretion of purine derivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. Br. J. Nutr. 63:131-142.

3.
Fujihara, T., E. R. Orskov, P. J. Reeds and D. J. Kyle. 1987. The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. J. Agric. Sci. (Camb). 107:12.

4.
Goering, H. K. and P. J. VanSoest. 1970. Forage fiber analysis. In: Agriculture Handbook No. 379. Agriculture Research Services. Washington, DC. US.

5.
Jones, G. A. and J. D. Milligan. 1975. Influence on some rumen and blood parameter of feeding acetohydroxamic acid in a urea-containing ration for lambs. Can. J. Anim. Sci. 55:39-47.

6.
Khandaker, Z. H. and A. M. M. Tareque. 1997. Effect of rumen degradable protein in straw based ration on purine derivatives excretion and microbial nitrogen supply in cattle. Asian-Aus. J. Anim.Sci. 10: 364-370.

7.
Ludden, P. A., D. L. Harmon, B. T. Larson and D. E. Axe. 2000a. Influence of the novel urease inhibitor N-(n-butyl) thiophosphoric triamide on ruminant nitrogen metabolism:ΙIn vitro urea kinetics and substrate digestion. J. Anim. Sci. 78:181-187.

8.
Ludden, P. A., D. L. Harmon, B. T. Larson and D. E. Axe. 2000b. Influence of the novel urease inhibitor N-(n-butyl) thiophosphoric triamide on ruminant nitrogen metabolism:Ⅱ. Ruminal nitrogen metabolism, diet digestibility and nitrogen balance in lambs. J. Anim. Sci. 78:188-197.

9.
Marsh, W. H., B. Fingerhut and H. Miller. 1965. Automated and manual direct methods for determination of blood urea. Clinical Chem. 11:624-627.

10.
Moore, M. J., W. R. Woods and T. J. Kopfenstein. 1968. Nitrogen metabolism as influenced by acetohydroxamic acid. J. Anim. Sci. 27:1172-1173.

11.
SAS. 1990. SAS/STAT User' Guide. Volume 2, Version 6, Fourth Edition. SAS Institute Inc., SAS Campus Drive, Cary, NC27513.

12.
Satter, L. D. and L. L. Slyter. 1974. Effect of feeding urea supplemented and urea-treated wheat straw on rumen fermentation pattern and fiber digestibility in crossbred cattle. Indian J. Anim. Nutr. 7(2):107-112.

13.
Streeter, C. L., R. R. Oltjen, L. L. Slyter and W. N. Fishbein. 1969. Urea utilization in wethers receiving the urease inhibitor acetohydroxamic acid. J. Anim. Sci. 29:88-93.

14.
Topps, J. H. and R. C. Elliott. 1965. Relationship between concentrations of ruminal nucleic acids and excretion of purine derivatives by sheep. Nature 205:498-499. crossref(new window)

15.
Voigt, J., K. Krawielitzki and B. Piatkowski. 1980b. Studies on the effect of phosphoric phenly ester diamide as inhibitor of the rumen urease of dairy cows:2. The metabolism of 15Nurea. Arch.Tierernaehr. 30:825-834. crossref(new window)

16.
Voigt, J., K. Krawielitzki and B. Piatkowski. 1980c. Studies on the effect of phosphoric phenly ester diamide as inhibitor of the rumen urease of dairy cows: 3. Digestibility of the nutrients and bacterial protein synthesis. Arch. Tierernaehr. 30:835-840. crossref(new window)

17.
Voigt, J., K. Krawielitzki and B. Piatkowski. 1980a. Studies on the effect of phosphoric phenly ester diamide as inhibitor of the rumen urease of dairy cows: 1. Influence of urea hydrolysis ammonia release and fermentation in the rumen. Arch. Tierernaehr. 30:811-823. crossref(new window)

18.
Verbic, J., X. B. Chen, N. A. Macleod and E. R. Orskov. 1990. Excretion of purine derivatives by ruminants: effect of microbial nucleic acid infusion on purine derivative excretion by steers. J. Agric. Sci. Camb. 114:243-248. crossref(new window)

19.
Whitelaw, F. G., J. S. Milne and S. A. Wright. 1991. Urease inhibition in the sheep rumen and its effect on urea and nitrogen metabolism. Br. J. Nutr. 66:209-225. crossref(new window)

20.
Young, E. G. and C. F. Conway. 1942. On the estimation of allantoin by Rimini-Schryner reaction. J. Biol. Chem. 142:839-853.

21.
Zhang, Y. G., A. S. Shan and J. Bao. 2001. Effect of hydroquinone on ruminal urease in the sheep and its inhibition kinetics in vitro. Asian-Aust. J. Anim. Sci. 14:1216-1220.