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Role of Diacyl Glycerol (DAG) in Caprine Sperm Acrosomal Exocytosis Induced by Progesterone
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Role of Diacyl Glycerol (DAG) in Caprine Sperm Acrosomal Exocytosis Induced by Progesterone
Somanath, P.R.; Gandhi, K.K.;
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Capacitated goat spermatozoa generated diacyl glycerol (DAG) when suspended in Krebs-Ringer bicarbonate medium and induced by progesterone or ionophore A23187. We have added Sn-1-oleoyl-2-acetyl glycerol externally, to study the effect of DAG in goat sperm acrosomal exocytosis. Addition of neomycin abolished the DAG generating capacity of progesterone in a dose dependent manner, suggesting the involvement of a phosphoinositidase C activated phospholipase C system in the process. The level of increase in phosphatidic acid was considerably low and was produced well after the DAG generation thereby suggesting the involvement of a DAG kinase which phosphorylates DAG to produce PA. The inhibition of progesterone mediated effect by inhibitors of channel and channels further supports the evidence that the events of binding of agonist to the receptor(s), opening of channels and the activation of phospholipase C are reconciled to perform the function of acrosome reaction in capacitated goat spermatozoa.
Goat;Spermatozoa;Acrosome Reaction;Diacyl Glycerol;Phospholipase C;
 Cited by
Austin, C. R. 1951. Observation on the penetration of sperm into the mammalian egg. Aust. J. Sci. Res. B4:697-698.

Baldi, E., R. Casano, C. Falcetti, C. Krausz, M. Maggi and G. Forti. 1991. Intracellular Ca$^{2+}$ accumulation and responsiveness to progesterone in capacitating human spermatozoa. J. Androl. 12:323-330.

Bansal, P. and S. K. Atreja. 1991. Phosphoinositide specific phospholipase C activity of goat spermatozoa in transit from the caput to the Cauda epididymis. Indian J. Biochem. Biophys. 28:307-311.

Berridge, M. J. 1987. Inositol triphosphate and diacyl glycerol : Two interacting second messengers. Rev. Biochem. 56:159-193. crossref(new window)

Blackmore, P., S. Beebe, D. Danforth and N. Alexander. 1990. Progesterone and 17 hydroxy progesterone, novel stimulators of calcium influx in human sperm. J. Biol. Chem. 265:1376-1380.

Bligh, E. G. and W. J. Dryer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917.

Bray, G. A. 1960. A simple efficient liquid scintillation for counting aqueous solutions in a liquid scintillation counter. Anal. Biochem. 1:279-285. crossref(new window)

Carney, D. H., D. L. Scott, E. A. Gordon and E. F. La Belle. 1985. Phosphoinositides in mitogenesis : neomycin inhibits thrombin-stimulated phosphoinositide turnover and initiation of cell proliferation. Cell. 42:479-488. crossref(new window)

Chang, M. C. 1951. Fertilizing capacity of spermatozoa deposited in the fallopian tubes. Nature 168:697-689. crossref(new window)

Cockcroft, S. and B. D. Gomperts. 1985. Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase. Nature 314:534-536. crossref(new window)

Cross, N. L., P. Morales, J. M. Overstreet and F. W. Hanson. 1988. Induction of acrosome reaction by human zona pellucida. Biol. Reprod. 38:235-244. crossref(new window)

De Chaffong de Courcellas, D., P. Rocvens and H. Van Belle. 1989. The role of endogenously formed diacyl glycerol in the propagation and termination of platelet activation. A biochemical and functional analysis using the novel diacyl glycerol kinase inhibitor R59949. J. Biol. Chem. 264:3247-3285.

Domino, S. E., S. B. Bockino and D. L. Garbers. 1989. Activation of phospholipase D by the fucose-sulfate glyco conjugate that induces an acrosome reaction in spermatozoa. J. Biol. Chem. 264:9412-9419.

Exton, J. H. 1990. Signalling through phosphatidylcholine breakdown. J. Biol Chem. 265:1-4.

Exton, J. H. 1994. Phosphatidyl choline breakdown and signal transduction. Biochim. Biophys. Acta. 1212:26-42.

Kaul, G., S. Singh, K. K. Gandhi and S. R. Anand. 1997. Calcium requirement and time course of capacitation of goat spermatozoa assessed by chlortetracycline assay. Andrologia 24:243-251.

Meizel, S. and K. O. Turner. 1991. Progesterone acts at the plasma membrane of human sperm. Mol. Cell. Endocrinol. 11:R1-R5. crossref(new window)

Meizel, S., M. Pillai, E. Diaz-Perez and P. Thomas. 1990. Initiation of human sperm acrosome reaction by components of human follicular fluid and cumulus secretions including steroids. In: Fertilization in Mammals (Ed. B. D. Bavister, J. M. Cummins, E. R. S. Roldan). Serona Symposia, Norwell MA, pp. 205-222.

Melendrez, C., S. Meizel and R. Berger. 1994. Comparison of the ability of progesterone and heat solubilized porcine zona pellucida to initiate the porcine sperm acrosome reaction. Mol. Reprod. Dev. 39:433-438. crossref(new window)

Meyers, S. A., K. M. Irwain, J. W. Overstreet, S. Wadas and E. Z. Drobniz. 1996. Zona pellucida binding and zona induced acrosome reaction in horse spermatozoa : comparison between fertile and infertile stallions. Theriogenology 46:1277-1288. crossref(new window)

Meyers, S. A., J. W. Overstreet, I. K. M. Liu and E. Z. Drobniz. 1995. Capacitation in vitro of stallion spermatozoa : comparisons of progesterone induced acrosome reactions in fertile and subfertile males. J. Androl. 16:47-54.

Mukerjee, A. B. and J. Lippes. 1972. Effect of human follicular and tubal fluids on human, mouse and rat spermatozoa in vitro. Can. J. Genet. Cytol. 14:167-174.

O'Toole, C. M. B., E. R. S. Roldan and L. R. Fraser. 1996a. Role of Ca$^{2+}$ channels in the signal transduction pathway leading to acrosomal exocytosis in human spermatozoa. Mol. Reprod. Dev. 45:204-211. crossref(new window)

O'Toole, C. M. B., E. R. S. Roldan, P. Hampton and L. R. Fraser. 1996b. A role of diacyl glycerol in human sperm acrosomal exocytosis. Mol. Hum. Reprod. 2:317-326. crossref(new window)

Osman, R. A., M. L. Andria, A. D. Jones and S. Meizel. 1989. Steroid induced exocytosis : the human sperm acrosome reaction. Biochem. Biophys. Res. Commun. 160:828-833. crossref(new window)

Revelli, A., M. Modotti, A. Pittaretti-Yanez, M. Massobrio and M. Balerna. 1994. Steroid receptors in human spermatozoa. Hum. Reprod. 9:760-766.

Roldan, E. R. S. and E. Dawes. 1993. Phospholipase D and exocytosis of the ram sperm acrosome. Biochim. Biophys. Acta. 1210:48-54.

Roldan, E. R. S. and R. A. P. Harrison. 1989. Polyphosphoinositide breakdown and subsequent exocytosis in the Ca$^{2+}$ ionophore induced acrosome reaction of mammalian spermatozoa. Biochem. J. 259:397-406.

Roldan, E. R. S. and T. Murase. 1994. Polyphosphoinositidederived diglycerol stimulates the hydrolysis of phosphatidyl choline by phospholipase C during exocytosis of the ram sperm acrosome. J. Biol. Chem. 269:23583-23589.

Schacht, J. 1978. Purification of polyphosphoinositides by chromatography or immobilized neomycin. J. Lipid Res. 19:1063-1067.

Siiteri, J. E., P. Dandekar and S. Meizel. 1988. Human sperm acrosome reaction - initiating activity associated with the human cumulus oophorus and murine granulosa cells. J. Exp. Zool. 246:71-80. crossref(new window)

Snedecor, G. W. and W. G. Cochran. 1967. Statistical methods. Oxford & IBH Publishing Co., Calcutta.

Somanath, P. R. and K. K. Gandhi. 2002. Role of calcium and calcium channels in progesterone induced acrosome reaction in caprine spermatozoa. Asia-Aust. J. Anim. Sci. 15(7):949-956.

Somanath, P. R., K. Suraj and K. K. Gandhi. 2000. Caprine sperm acrosome reaction: Promotion by progesterone and homologous zona pellucida. Small Rum. Res. 37:279-286. crossref(new window)

Tesarik, J. 1985. Comparison of acrosome reaction - inducing activities of human cumulus oophorus, follicular fluid and ionophore A23187 in human sperm populations of proven fertility ability in vitro. J. Reprod. Fertil. 74:383-388. crossref(new window)

Thomas, P. and S. Meizel. 1989. Phosphatidyl inositol 4,5-bisphosphate hydrolysis in human sperm stimulated with follicular fluid or progesterone is dependent upon Ca$^{2+}$ influx. Biochem. J. 264:539-546.