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Postnatal Expression of Growth/Differentiation Factor-8 (GDF-8) Gene in European and Asian Pigs
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 Title & Authors
Postnatal Expression of Growth/Differentiation Factor-8 (GDF-8) Gene in European and Asian Pigs
Lin, C.S.; Wu, Y.C.; Sun, Y.L.; Huang, M.C.;
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 Abstract
Myostatin (growth differentiation factor (GDF)-8), is one member of the transforming growth factor superfamily. Investigations of GDF-8 null mice and double-muscled cattle revealed that GDF-8 has a profound influence upon skeletal muscle growth. Therefore, the GDF-8 effect upon the productive performance of pigs is worth exploring. In the present study, the nucleotide sequences and expression levels of GDF-8 genes in European pigs (Landrace and Duroc) and Asian pigs (Taoyuan and Small-ear) were evaluated. Based upon their genetic background these breeds possess significantly distinct growth rate and muscle productionphenotypes. Our sequence data showed that the nucleotide sequences of European and Asian pigs were 100% similar. Postnatal expression of GDF-8 gene in skeletal muscles, from birth to 12 mo of age, among different breeds was measured. GDF-8 expression levels in the longissimus muscle of neonatal European breed littermates were the highest, however it declined significantly (p<0.05) at 1 and 3 mo, and then increased gradually at 6 to 12 mo. The Asian breeds, however, GDF-8 expression level increased markedly at 3 mo and maintained a constant level thereafter. The results indicate that rather than polymorphism within the GDF-8 functional sequence between European and Asia breeds, it was relative to the gene regulation in postnatal muscle growth.
 Keywords
Growth Differentiation Factor-8;Skeletal Muscle;Pig;Gene Expression;Myostatin;
 Language
English
 Cited by
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 References
1.
Devereux, J., P. Haeberli and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395. crossref(new window)

2.
Dunner, S., C. Charlier, F. Farnir, B. Brouwers, J. Canon and M. Georges. 1997. Towards interbreed IBD fine mapping of the mh locus: double-muscling in the Asturiana de los Valles breed involves: the same locus as in Belgian Blue cattle breed. Mamm. Genome 8:430-435. crossref(new window)

3.
Gaur, G. K., S. P. S. Ahlawat, A. K. Chhabra and S. Paul. 1998. Genetic trend for growth in a closed Indian herd of Landrace x desi crossbreds. Asian-Aust. J. Anim. Sci. 11:363-367.

4.
Griffith, D. L., P. C. Keck, T. K. Sampath, D. C. Rueger and W. D. Carlson. 1996. Three-dimensional structure of recombinant human osteogenic protein 1: structural paradigm for the transforming growth factor-beta superfamily. Proc. Natl. Acad. Sci. USA 93:878-883. crossref(new window)

5.
Grobet, L., L. J. R. Martin, D. Poncelet, D. Pirottin, B. Brouwers, J. Riquet, A. Schoeberlein, S. Dunner, F. Menissier, J. Massabanda, R. Fries, R. Hanset and M. Georges. 1997. A deletion in the bovine myostatin gene causes the doublemuscled phenotype in cattle. Nat. Genet. 17:71-74. crossref(new window)

6.
Hyun, Y., B. F. Wolter and M. Ellis. 2001. Feed intake patterns and growth performance of purebred and crossbred Meishan and Yorkshire pigs. Asian-Aust. J. Anim. Sci. 14:837-843.

7.
Ji, S., R. L. Losinski, S. G. Cornelius, G. R. Frank, G. M. Willis, D. E. Gerrard, F. F. S. Depreux and M. E. Spurlock. 1998. Myostatin expression in porcine tissues: tissue specificity and developmental and postnatal regulation. Am. J. Physiol. 44:R1265-1273.

8.
Kambadur, R., M. Sharma, T. P. L. Smith and J. J. Bass. 1997. Mutations in myostatin (GDF-8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Res.7:910-916.

9.
Kingsley, D. M. 1994. The TGF-beta superfamily: new members, new receptors, and new genetic tests of function in different organisms. Genes Dev. 8:133-146. crossref(new window)

10.
Lee, S. J. and A. C. McPherron. 1999. Myostatin and the control of skeletal muscle mass. Curr. Opin. Genet. Dev. 9:604-607. crossref(new window)

11.
McPherron, A. C. and S. L. Lee. 1996. In growth factors and cytokines in health and disease, eds. LeRoith, D. and C. Bondy (JAI, Greenwich, CT), Vol. 1B, pp.357-393.

12.
McPherron, A. C. and S. J. Lee. 1997. Double muscling in cattle due to mutations in the myostatin gene. Proc. Natl. Acad. Sci. USA 94:12457-12461. crossref(new window)

13.
McPherron, A. C., A. M. Lawler and S. J. Lee. 1997. Regulation of skeletal muscle mass in mice by a new TGF-$\beta$ superfamily member. Nature 387:83-90. crossref(new window)

14.
National Science Council. 1993. Handbook of management and use in laboratory animal. Taipei, Taiwan.

15.
Serra, J. J., M. Ellis and C. S. Haley. 1992. Genetic components of carcass and meat quality traits in Meishan and Large White pigs and their reciprocal crosses. Anim. Prod. 54:117-127.

16.
Stratil, A. and M. Kopecny. 1999. Genomic organization, sequence and polymorphism of the porcine myostatin (GDF8; MSTN) gene. Anim. Genet. 30:462-478. crossref(new window)

17.
Young, L. D. 1992. Effects of Duroc, Meishan, Fengjing, and Minzhu boars on productivity of mates and growth of firstcross progeny. J. Anim. Sci. 70:2020-2029.