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Postnatal Expression of Growth/Differentiation Factor-8 (GDF-8) Gene in European and Asian Pigs
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 Title & Authors
Postnatal Expression of Growth/Differentiation Factor-8 (GDF-8) Gene in European and Asian Pigs
Lin, C.S.; Wu, Y.C.; Sun, Y.L.; Huang, M.C.;
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Myostatin (growth differentiation factor (GDF)-8), is one member of the transforming growth factor superfamily. Investigations of GDF-8 null mice and double-muscled cattle revealed that GDF-8 has a profound influence upon skeletal muscle growth. Therefore, the GDF-8 effect upon the productive performance of pigs is worth exploring. In the present study, the nucleotide sequences and expression levels of GDF-8 genes in European pigs (Landrace and Duroc) and Asian pigs (Taoyuan and Small-ear) were evaluated. Based upon their genetic background these breeds possess significantly distinct growth rate and muscle productionphenotypes. Our sequence data showed that the nucleotide sequences of European and Asian pigs were 100% similar. Postnatal expression of GDF-8 gene in skeletal muscles, from birth to 12 mo of age, among different breeds was measured. GDF-8 expression levels in the longissimus muscle of neonatal European breed littermates were the highest, however it declined significantly (p<0.05) at 1 and 3 mo, and then increased gradually at 6 to 12 mo. The Asian breeds, however, GDF-8 expression level increased markedly at 3 mo and maintained a constant level thereafter. The results indicate that rather than polymorphism within the GDF-8 functional sequence between European and Asia breeds, it was relative to the gene regulation in postnatal muscle growth.
Growth Differentiation Factor-8;Skeletal Muscle;Pig;Gene Expression;Myostatin;
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