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RAPD Fingerprinting for the Species Identification of Animals
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 Title & Authors
RAPD Fingerprinting for the Species Identification of Animals
Huang, Mu-Chiou; Horng, Yan-Ming; Huang, Hsiu-Lin; Sin, Yen-Long; Chen, Ming-Jaw;
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The studies were based on the RAPD fingerprinting for the species identification of animals. The genomic DNA samples of ostriches, Taiwan local chickens, Aboracres broilers, Leghorn chickens, quails, doves, emus, Beltville small white turkeys, pheasants, Chinese geese, mule ducks, Holstein cattle and Landrace pigs were amplified with random primers by RAPD-PCR for fingerprinting. The results showed that the varied band patterns of DNA fingerprints were generated from templates depending on the kinds of primers or animal species. The same primer applied to the same breed, all of the main bands are similar, but which were different among species. In order to try to identify the species from the mixture of meat by RAPD fingerprinting, the meat of ostrich and cattle was mixed in different ratios for this study. The results showed that it could be easily and precisely distinguished according to the band distribution of RAPD patterns.
Genomic DNA;RAPD-PCR;Polymorphism;Fingerprinting;Species Identification;
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아세아태평양축산학회지, 2008. vol.21. 9, pp.1233-1237 crossref(new window)
A novel sex-specific DNA marker in Columbidae birds, Theriogenology, 2007, 67, 2, 328  crossref(new windwow)
Identification of Species Origin of Meat and Meat Products on the DNA Basis: A Review, Critical Reviews in Food Science and Nutrition, 2015, 55, 10, 1340  crossref(new windwow)
Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species, Genetics and Molecular Research, 2011, 10, 3, 1416  crossref(new windwow)
Application of a real-time PCR assay for the detection of ostrich (Struthio camelus) mislabelling in meat products from the retail market, Food Control, 2011, 22, 3-4, 523  crossref(new windwow)
A review of current PCR-based methodologies for the authentication of meats from game animal species, Trends in Food Science & Technology, 2010, 21, 8, 408  crossref(new windwow)
Identification of pork genome in commercial meat extracts for Halal authentication by SYBR green I real-time PCR, International Journal of Food Science & Technology, 2011, 46, 5, 951  crossref(new windwow)
A Simple and Rapid PCR-Based Method for Ostrich Sexing Using Micro Amounts of DNA, Reproduction in Domestic Animals, 2008, 43, 6, 643  crossref(new windwow)
A novel molecular genetic marker for gender determination of pigeons, Theriogenology, 2006, 65, 9, 1759  crossref(new windwow)
RAPD analysis as a tool for discriminating marble trout from hybrids (marble trout x brown trout) in the zones of hybridization, Journal of Animal Breeding and Genetics, 2004, 121, 3, 156  crossref(new windwow)
Andrews, C. D., R. G. Berger, R. P. Mageau, B. Schwab and R. W. Johnston. 1992. Detection of beef, sheep, deer, and horse meat in cooked meat products by enzyme-linked immunosorbent assay. J. AOAC. Int. 75:572-576.

Appannavar, M. M., M. G. Govindaiah and K. P. Ramesha. 2003. Genetic distance study among deoni breed of cattle using random amplified DNA markers. Asian-Aust. J. Anim. Sci. 16:315-319.

Chikuni, K., K. Ozutsumi, T. Koishikawa and S. Kato. 1990. Species identification of cooked meats by DNA hybridization assay. Meat Sci. 27:119-128.

Chikuni, K., T. Tabata, M. Kosugiyama, M. Monma and M. Saito. 1994. Polymerase chain reaction assay for detection of sheep and meats. Meat Sci. 37:337-345.

Choy, Y. H., S. J. Oh and J. O. Kang. 2001. Application of RAPD method in meat for beef breed identification. Asian-Aust. J. Anim. Sci. 14:1655-1658.

Dincer, B., J. L. Spearow, R. G. Cassens and M. L. Greaser. 1987. The effects of curing and cooking on the detection of species origin of meat products by competitive and indirect ELISA techniques. Meat Sci. 20:253-260.

Horng, Y. M. and M. C. Huang. 1999. Comparison between RAPD and RAMPO fingerprints of Holstein cattle. J. Agri. Assoc. China. 186:134-143.

Horng, Y. M. and M. C. Huang. 2000. Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Proc. Natl. Sci. Counc. ROC (B) 24:41-46.

Horng, Y. M. and M. C. Huang. 2003. Male-specific DNA sequences in pigs. Theriogenology 59:841-848.

Huang, M. C. 1988. Studies on the microinjection of foreign gene into mammalian fertilized eggs. J. Agri. Forest. 37:97-109.

Huang, M. C., W. C. Lin, Y. M. Horng, R. Rouvier and C. W. Huang. 2003. Female-specific DNA sequences in geese. Br. Poult. Sci. 44:1-6.

Joens, S. J. and R. L. S. Patterson. 1985. Double-antibody ELISA for detection of trace amounts of pig meat in raw meat mixtures. Meat Sci. 15:1-13.

Kang’ethe, E. K., J. M. Gathuma and K. J. Lindqvist. 1986. Identification of the species of origin of fresh, cooked and canned meat on meat products using antisera to thermostable muscle antigens by Ouchterlony’s double diffusion test. J. Sci. Food Agric. 37:157-162.

King, N. L. 1984. Breakdown of connection during cooking of meat. Meat Sci. 11:27-43. crossref(new window)

Martin, G. B., J. G. K. Williams and S. D. Tanksley. 1991. Rapid identification of marker linked to a pseudomonas resistance gene in tomato using random primers and near-isogenic lines. Proc. Natl. Acad. Sci. USA 88:2336-2340.

Patterson, R. M., R. G. Whittaker and T. L. Spencer. 1984. Improved species identification of raw meat by double sandwich enzyme-linked immunosorbent assay. J. Sci. Food Agric. 35:1018-1023.

Sensi, E., R. Vignani, M. Scali, E. Masi and M. Cresti. 2003. DNA fingerprinting and genetic relatedness among cultivated varieties of Olea europaea L. estimated by AFLP analysis. Sci. Hort. 97:379-388.

Shiau, J. W. and M. C. Huang. 1997. The probe of repetitive sequence for DNA fingerprinting in Holstein cattle. J. Agri. Assoc. China. 177:1-10.

Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.

Wintero, A. K., P. D. Thomsen and W. Davies. 1990. A comparison of DNA hybridization, immunodiffusion, countercurrent immunoelectrophoresis and isoelectric focusing for detecting the admixture of pork to beef. Meat Sci. 27:75-85.

Yen, N. T., M. C. Huang and C. Tai. 2001. Genetic variation of RAPD polymorphisms in Taoyuan and Duroc pigs. J. Anim. Breed. Genet. 118:111-118.

Zerifietal, A., C. Labie and G. Bernard. 1992. SDS-PAGE technique for the species identification of cooked meat. Fleischwirtsch Int. 1: 54-56.