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Post-thaw Thermal Resistance Test on Motility and Acrosomal Integrity of Filtered and Non-filtered Frozen Semen of Murrah Buffalo Bulls
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 Title & Authors
Post-thaw Thermal Resistance Test on Motility and Acrosomal Integrity of Filtered and Non-filtered Frozen Semen of Murrah Buffalo Bulls
Maurya, V.P.; Tuli, R.K.;
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Present investigation was conducted to determine the post-thaw sperm motility and acrosomal damage of filtered and non-filtered frozen semen of Murrah buffalo bulls. Twenty semen ejaculates (from four Murrah buffalo bulls collected at weekly interval) were diluted in Tris egg yolk glycerol extender and divided into two parts. One was filtered through sephadex G-100 column and the other portion was kept as such (non-filtered). Both fractions were frozen in liquid nitrogen () by the standard method developed in the laboratory. After 24 h of freezing, non-filtered and filtered semen samples were thawed at for 1 min. These samples were incubated at in a water both. The different seminal characteristics i.e. percent progressive sperm motility, live and abnormal spermatozoa and spermatozoa with damaged acrosome were assessed at hourly interval till they remained motile. The filtered frozen and thawed semen showed significantly (p<0.05) high sperm viability and acrosomal integrity as compared to non-filtered semen.
Murrah Bull;Sephadex Filtration;Frozen Semen Motility;Acrosomal Integrity;
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Influence of selected factors on bovine spermatozoa cold shock resistance, Acta Veterinaria Brno, 2015, 84, 2, 125  crossref(new windwow)
Anzar, M. and E. F. Graham. 1995. Role of sperm motility and acrosomal integrity in the filtration of bovine semen. Theriogenology 43:513-520.

Anzar, M., E. F. Graham and N. Iqbal. 1997. Post-thaw plasma membrane integrity of bull spermatozoa separated with sephadex column. Theriogenology 47:845-856.

Campbell, R. C., J. L. Hancock and I. G. Shaw. 1960. Cytological characteristics and fertilizing capacity of bull spermatozoa. J. Agric. Sci. 55:91-99.

Chauhan, S. S., G. Mohan, S. Kumar and K. L. Sahni. 1993. Effect of sephadex filtration in improving the quality of buffalo semen during storage and freezing. Indian. J. Anim. Sci. 63:1036-1041.

Cummins, J. M., A. M. Jequier and R. Kan. 1994. Molecular biology of human male fertility: link with aging, mitochondrial genetics and oxidative stress. Mol. Reprod. Dev. 37:345-362.

Dhami, A. J., V. R. Jani, K. L. Sahni. and G. Mohan. 1995. Freezability and fertility of heterospermic semen of Friesian and Murrah bulls using Tris and milk extenders. Indian Vet. J. 72:1273-1276.

Dhami, A. J., K. L. Sahni and G. Mohan. 1992. Effect of various cooling rates (from 30$^{\circ}C$ to 5$^{\circ}C$) and thawing temperatures on the deep freezing of Bos Taurus and Bos Bubalis semen. Theriogenology 38:565-574.

Fayemi, E. O., B. G. Crabo and E. F. Graham. 1979. Assay of frozen boar semen with sephadex filtration. Theriogenology 12:13-17.

Fiser, P. S., C. Hansen, H. Underhill and G. J. Marcus. 1991. New thermal test to assess the viability of cryopreserved boar sperm. Cryobiology 28:454-459.

Goyal, R. L. 1993. Effect of separation procedures on freezability of buffalo spermatozoa. Ph.D. Thesis, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.

Goyal, R. L., R. K. Tuli, G. C. Georgie and D. Chand. 1996. Comparison of quality and freezability of water buffalo semen after washing or sephadex filtration. Theriogenology 46:679-686.

Graham, E. F. and B. G. Crabo. 1982. The influence of dead or abnormal sperm on fertility. Adv. Anim. Breed. 1:12-13.

Graham, E. F. and J. K. Graham. 1990. The effect of whole ejaculate filtration on the morphology and fertility of bovine semen. J. Dairy Sci.73:91-97.

Graham, E. F., M. K. L. Schmehl and B. K. Evensen. 1978. An over view of column separation of spermatozoa. Proc. of the 7th NAAB Technical Conf. on Artificial Insemination and Reproduction, 13-15 April, Madision, WI, National Association of Animal Breeders, Columbia, MO, pp. 69-73.

Heuer, C. and M. N. Tahir. 1982. Experiment on deep freeze preservation of water buffalo semen using sephadex filtration as semen assay. Proc. Buffalo Seminar on Reproduction and Meat Production Tanuku: pp.55-63.

Kanakaraj, P., B. M. Easwaran and A. R. Krishnan. 1996. Sephadex column filtration in improving the quality of bull semen. Int. J. Anim. Sci. 11:125-127.

Kumar, N. 1989. Studies on filtration through different media and freezing of buffalo bull semen in certain extenders. M. V. Sc. Thesis, Punjab Agricultural University, Ludhiana, India.

Kumar, N., G. R. Pangawkar, R. K. Sharma and J. S. Matharoo. 1999. Effect of sephadex filtration of buffalo bull semen on freezability and sperm penetration into homologous cervical mucus. Indian J. Anim. Sci. 69:91-93.

Landa, C. A., J. O. Almquist and R. P. Amann. 1980. Factors influencing sephadex separation of bovine and ovine spermatozoa. J. Dairy Sci. 63:277-282.

Linford, E., F. A. Glover, C. Bishop and D. L. Stewart. 1976. The relationship between semen evaluating methods and fertility in the bulls. J. Reprod. Fertil. 47:283-291.

Maki-Laurila, M. and E. F. Graham. 1968. Separation of dead and live spermatozoa in bovine semen. J. Dairy Sci. 51:965.

Maurya, V. P., R. K. Tuli and R. L. Goyal. 2003. Effect of buffer composition, sephadex grade and column size on filtration based quality improvement of semen from Murrah buffalo bull. Asian-Aust. J. Anim. Sci. 16:165-171.

Norusis, M. J. 1988. SPSS/PC Student ware, SPSS Inc. Chicago, Illinois (USA).

Panghal, V. S. 1996. Freezability of buffalo semen after filtration through sephadex gel and glass wool columns. M.Sc. Thesis, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.

Panghal, V. S. and R. K. Tuli. 1999. Effect of filtration of Murrah buffalo semen on its freezability and seminal characters. Indian J. Dairy Sci. 52:233-236.

Raizada, S. 1979. Fertility of cattle and buffalo bulls vis-a-vis acrosomal abnormalities and rate of hyaluronidase release. M.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.

Rao, A. V. N., G. B. Harnath and G. Somasekharam. 1993. Revivability and acrosomal integrity of buffalo spermatozoa deep frozen in straws using different equilibration and thaw rates. Indian J. Anim. Res. 14:44-47.

Roberts, A. M. 1972. Gravitational separation of X- and Yspermatozoa. Nature 238:223-225. crossref(new window)

Saacke, R. G. 1970. Morphology of sperm and its relationship to fertility. Proc. NAAB III Tech, Conf. Artif. Insem. Reprod. pp. 17-30.

Saacke, R. G. and J. M. White. 1972. Semen quality test and their relationship to fertility. In: Proc. 4th NAAB Tech. Con. Artif. Insemination. Reprod. 18-20 April, Madison, W. I. National Association of Animal Breeders, Columbia, MO, pp. 22-27.

Samper, J. C. and B. G. Crabo. 1993. Assay of capacitated, freeze damaged and extended stallion spermatozoa by filtration. Theriogenology 39:1209-1220.

Shannon, P. and B. Curson. 1972. Toxic effect and action of dead sperm on diluted semen. J. Dairy Sci. 55:615-620.

Spreckels, I. 1994. Investigations on the selection of spermatozoa by glass wool sephadex filtration of stallion semen with special reference to cryopreservation. Ph.D. Thesis, University of Hannover, Germany.

Viswanath, R. and P. Shannon. 1997. Do sperm cell age? A review of physiological changes in sperm during storage at ambient temperature. Reprod. Fertil. Dev. 9:321-331.

Watson, P. F. and I. C. A. Martin. 1972. A comparison of change in the acrosomes of deep-frozen ram and bull spermatozoa. J. Reprod. Fertil. 28:99-102.

Younis, M., H. A. Samad, N. Ahmad and I. Ahmad. 1998. Studies on semen quality of young, adult and old buffalo bulls during low and peak breeding seasons. Pakistan Vet. J. 18:134-140.