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Effect of Feeding Chemically Treated Mustard (Brassica campestris) Straw on Rumen Fibre Degrading Enzymes in Sheep
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 Title & Authors
Effect of Feeding Chemically Treated Mustard (Brassica campestris) Straw on Rumen Fibre Degrading Enzymes in Sheep
Vaithiyanathan, S.; Raghuvanshi, S.K.S.; Mishra, A.S.; Tripathi, M.K.; Misra, A.K.; Prasad, R.; Jakhmola, R.C.;
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The aim of the experiment was to study the changes in the activities of various rumen fibre degrading enzymes due to the feeding of chemically treated mustard (Brassica campestris) straw in sheep. Mustard straw (MS) (<5 cm particle size) was treated either with urea (4% (w/w), or with 2% sodium hydroxide (NaOH), or with alkaline hydrogen peroxide (2% NaOH and 1.5% hydrogen peroxide ()) and/or supplemented with 2% (w/w) urea. Seven maintenance type rations were prepared using MS (70 parts) with molasses (5 parts) and concentrate (25 parts). They were untreated MS (CMS), urea treated MS (UMS), urea supplemented MS (MSUS), alkali treated MS (AMS), alkali treated and urea supplemented MS (AMS-US), alkali treated MS (AHMS) and alkali treated and urea supplemented MS (AHMS-US). They were then compressed into a complete feed block with the help of block making machine. Forty two male hoggets of Malpura breed sheep were equally distributed into each treatment group and (were) offered feed and water ad libitum. At the end of 21 days of feeding trial, rumen liquor was collected through stomach tube from three animals in each group at 0 h, 4 h, 8 h, 12 h of post feeding. Results showed that the level of enzyme varied from 8.52 to 11.12, 40.85 to 50.37, 3.22 to 3.78, 2.09 to 2.77 and 31.44 to 44.24 units/100 ml SRL respectively for carboxymethyl cellulase (CMCase), -amylase, microcrystalline cellulase (MCCase), filter paper (FP) degrading enzyme and -glucosidase. Processing of MS affected the enzyme activities, in a way, that NaOH and AHP treatment significantly reduced CMCase and FP degrading enzyme. The effect of urea treatment showed an increase in the activity of MCCase and -glucosidase. But the supplementation of urea increased the activity of CMCase, FP degrading enzyme and -glucosidase. The CMCase, -amylase, -glucosidase activities were highest at 4hr whereas MCCase and FP degrading enzyme had maximum activities at 12 h post feeding Results suggested that MS might need longer time in the rumen for its effective degradation.
Chemical Treatments;Mustard Straw;Rumen Fibre Degrading Enzyme;Sheep;
 Cited by
Optimization of Solid State Fermentation of Mustard (Brassica campestris) Straw for Production of Animal Feed by White Rot Fungi (Ganoderma lucidum),Misra, A.K.;Mishra, A.S.;Tripathi, M.K.;Prasad, R.;Vaithiyanathan, S.;Jakhmola, R.C.;

Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 2, pp.208-213 crossref(new window)
Agarwal, N. 2000. Estimation of fibre degrading enzyme. In: Feed microbiology. (Ed L. C. Chaudhary, N. Agarwal, D. N. Kamra amd D. K. Agarwal). CAS Animal Nutrition, IVRI, Izatnagar, India.

Agarwal, N., I. Agarwal, D. N. Kamra and L. C. Chaudhary. 2000. Diurnal variations in the activities of hydrolytic enzyme in different fractions of rumen content of Murrah Buffalo. J. Appl. Anim. Res. 18:73-80.

AOAC. 1995. Official methods of analysis. 16th ed., Association of Official Analytical Chemists Washington, DC.

Chaturvedi, O. H., A. Santra, A. S. Mishra, R. Prasad and R. C. Jakhmola. 1999. Effect of soaking medium and hours of soaking in nutritive value of mustard (Brassica campestris) straw. Indian J. Anim. Sci. 69:739-741.

Chaturvedi, O. H., A. Santra, A. S. Mishra, R. Prasad, A. K. Misra, S. Vaithiyanathan and R. C. Jakhmola. 2002. Titrable groups and soluble phenolics compounds in untreated and treated mustard (Brassica campestris) straw and their relation with cell wall constituents and dry matter disappearance. Indian J. Anim. Sci.71:69-73.

Chaudhry, A. S. 1998. Chemical and biological procedures to upgrade cereal straw for ruminants. Nutr. Abst and Rev. (Series B). 68:319-331.

Klopfenstein, T. J. 1978. Chemical treatment of crop residues. J. Anim. Sci. 46:841-848.

Miller, G. L. 1959. Modified DNS method for reducing sugars. Anal. Chem. 31:426-428. crossref(new window)

Mishra, A. S., O. H. Chaturvedi, A. K. Misra and S. A. Karim. 2000. Comparative utilization of urea and fungal treated MS by sheep. Indian J. Anim. Sci. 70:854-856.

Mishra, A. S., O. H. Chaturvedi, A. K. Misra and S. A. Karim. 1996. A note on chemical composition of untreated and urea treated mustard (Brassica campestris) straw. Indian J. Small Rumi. 2:49-51.

Misra, A. K., O. H. Chaturvedi, A. S. Mishra and S. A. Karim. 1999. Effect of urea treatment on the cell wall constituents and dry matter digestibility of mustard (Brassica campestris) straw. Indian J. Anim. Sci. 69:1180-1182.

Misra, A. K., S. A. Karim, D. L. Verma, A. S. Mishra and M. K. Tripathi. 2000. Nutrient intake, its utilization, rumen fermentation pattern and blood bio-chemical constituents of sheep fed urea treated mustard (Brassica campestris) straw. Asian-Aus. J. Anim. Sci. 13:1674-1680.

Robertson, J. B. and P. J. Van Soest. 1981. The detergent system of analysis and its application to human foods. Cornell University, Ithaca, New York.

Silva, A. T., R. J. Wallace and E. R. Orskov. 1987. Use of particle bound microbial activity to predict the rate and extent of fibre degradation: A review. Br. J. Nut. 57:407-415.

Tarkow, H. and W. L. Feist. 1969. A mechanism for improving the digestibility of lignocellulose materials with dilute alkali and liquid ammonia. J. Anim. Sci. 35:451-357.

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fibre, neutral detergent fibre and nonstarch polysaccharides in relation to animal nutrition. Symposium: Carbohydrate methodology, metabolism and nutritional implications in dairy cattle. J. Dairy Sci. 74:3583-3597.