JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Non-ionic Surfactants on Enzyme Distributions of Rumen Contents, Anaerobic Growth of Rumen Microbes, Rumen Fermentation Characteristics and Performances of Lactating Cows
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Non-ionic Surfactants on Enzyme Distributions of Rumen Contents, Anaerobic Growth of Rumen Microbes, Rumen Fermentation Characteristics and Performances of Lactating Cows
Lee, S.S.; Ahn, B.H.; Kim, H.S.; Kim, C.H.; Cheng, K.-J.; Ha, J.K.;
  PDF(new window)
 Abstract
A series of experiments was carried out to determine the possibility for the non-ionic surfactant (NIS) as a feed additive for ruminant animals. The effect of the NIS on (1) the enzyme distribution in the rumen fluids of Hereford bulls, (2) the growth of pure culture of rumen bacteria and (3) rumen anaerobic fungi, (4) the ruminal fermentation characteristics of Korean native cattle (Hanwoo), and (5) the performances of Holstein dairy cows were investigated. When NIS was added to rumen fluid at the level of 0.05 and 0.1% (v/v), the total and specific activities of cell-free enzymes were significantly (p<0.01) increased, but those of cell-bound enzymes were slightly decreased, but not statistically significant. The growth rates of ruminal noncellulolytic species (Ruminobacter amylophilus, Megasphaera elsdenii, Prevotella ruminicola and Selenomonas ruminantium) were significantly (p<0.01) increased by the addition of NIS at both concentrations tested. However, the growth rate of ruminal cellulolytic bacteria (Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens and Butyrivibrio fibrisolvens) were slightly increased or not affected by the NIS. In general, NIS appears to effect Gram-negative bacteria more than Gram-positive bacteria; and non-cellulolytic bacteria more than cellulolytic bacteria. The growth rates of ruminal monocentric fungi (Neocallimastix patriciarum and Piromyces communis) and polycentric fungi (Orpinomyces joyonii and Anaeromyces mucronatus) were also significantly (p<0.01) increased by the addition of NIS at all concentrations tested. When NIS was administrated to the rumen of Hanwoo, Total VFA and ammonia-N concentrations, the microbial cell growth rate, CMCase and xylanase activities in the rumen increased with statistical difference (p<0.01), but NIS administration did not affect at the time of 0 and 9 h post-feeding. Addition of NIS to TMR resulted in increased TMR intake and increased milk production by Holstein cows and decreased body condition scores. The NEFA and corticoid concentrations in the blood were lowered by the addition of NIS. These results indicated that the addition of NIS may greatly stimulate the release of some kinds of enzymes from microbial cells, and stimulate the growth rates of a range of anaerobic ruminal microorganisms, and also stimulate the rumen fermentation characteristics and animal performances. Our data indicates potential uses of the NIS as a feed additive for ruminant animals.
 Keywords
Non-ionic Surfactants;Enzyme Activity;Microbial Growth;Rumen Microorganism;Ruminal Fermentation;Lactation;Performances;Hanwoo;Holstein;
 Language
English
 Cited by
1.
Influences of Surfactant Tween 80 on the Gas Production, Cellulose Digestion and Enzyme Activities by Mixed Rumen Microorganisms,;;

Asian-Australasian Journal of Animal Sciences, 2003. vol.16. 8, pp.1151-1157 crossref(new window)
2.
Effects of Tween 80 Pretreatment on Dry Matter Disappearance of Rice Straw and Cellulolytic Bacterial Adhesion,;;;;;;;

Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 9, pp.1397-1401 crossref(new window)
3.
Effects of Mixtures of Tween80 and Cellulolytic Enzymes on Nutrient Digestion and Cellulolytic Bacterial Adhesion,;;;;;;;;;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 11, pp.1604-1609 crossref(new window)
4.
비이온성 및 양쪽 이온성 계면활성제 첨가가 반추위 혼합 미생물의 성장과 볏짚의 in vitro 소화에 미치는 영향,이신자;김완영;문여황;김현섭;김경훈;하종규;이성실;

생명과학회지, 2008. vol.18. 4, pp.515-521 crossref(new window)
 References
1.
Allison, M. J. 1978. Production of branched-chain volatile fatty acids by certain anaerobic bacteria. Appl. Environ. Microbiol. 35:872-877.

2.
Asther, M., G. Corrieu, R. Drapon, and E. Odier. 1987. Effect of Tween 80 and oleic acid on ligninase production by Phanerochaete chrysosporium INA-12. Enzyme Microbiol. Technol. 9:245-249. crossref(new window)

3.
Brown, M. R. W., and R. M. E. Richards. 1964. Effect of polysorbate (Tween) 80 on the resistance of Pseudomonas aeruginosa to chemical inactivation. J. Pharm. Pharmacol. 16:51-55.

4.
Bryant, M. P., and L. A. Burkey. 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205-217.

5.
Caldwell, D. R., and M. P. Bryant. 1966. Medium without rumen fluid for non-selective enumeration and isolation of rumen bacteria. Appl. Microbiol. 14:794-801.

6.
Dehority, B. A., and H. W. Scott. 1967. Extent of cellulose and hemicellulose digestion in various forages by pure cultures of rumen bacteria. J. Anim. Sci. 52:418-426.

7.
Demain, A. L., and J. Birnbaum. 1968. Alternation of permeability for the release of metabolites from microbial cells. Curr. Topics Microbiol. Immun. 46:1-25.

8.
Deshpande, M. V., M. C. Srinavasan, and S. S. Deshmakh. 1987. Effects of fatty acids on cellulase production by Penicillium funiculosum and its mutants. Biotechnol. Lett. 9:301-304. crossref(new window)

9.
Graham, H., P. Aman, O. Theander, N. Kolankaya, and C. S. Stewart. 1985. Influence of heat sterilisation and ammoniation on straw composition and degradation by pure cultures of cellulolytic rumen bacteria. Anim. Feed Sci. Technol. 12:195-203. crossref(new window)

10.
Halliwell, G., and M. P. Bryant. 1963. The cellulolytic activity of pure strains of bacteria from the rumen of cattle. J. Gen. Microbiol. 32:441-448.

11.
Heinrichova, K., M. Wojciechowiez, and A. Ziolecki. 1985. An exo-D-galacturonase of Butyrivibrio fibrisolvens from bovine rumen. J. Gen. Microbiol. 131:2053-2058.

12.
Hulme, M. A., and D. W. Stranks. 1970. Induction and the regulation of production of cellulase by fungi. Nature, London. 226:469-470. crossref(new window)

13.
Hungate, R. E. 1950. The anaerobic mesophyllic cellulolytic bacteria. Bacteriol. Rev. 14:1-49.

14.
Hung, B. R., L. Lara, M. A. Patron, N. N. Ugarova, W. Bechstedt, and S. Clappes. 1988. Tween 80 and proteose peptone effect on cellulase production. Acta Biotechnol. 8:461-464. crossref(new window)

15.
Long, K., and J. S. Knapp. 1991. The effect of Junlon PW110 and Tween 80 on the production of cellulolytic enzymes by Coprinus cinereus. Mycol. Res. 95:1077-1081. crossref(new window)

16.
Lowe, S. E., M. K. Theodorou, and A. P. J. Trinci. 1987. Isolation of anaerobic fungi from saliva and faeces of sheep. J. Gen. Microbiol. 133:1829-1834.

17.
Miller, G. L. 1959. Use of dinitrosalicylic acid as reagent for the determination of ruducing sugars. Anal. Chem. 31:426-428. crossref(new window)

18.
Morris, E. J., and N. P. Van Gylswyk. 1980. Comparison of the action of rumen bacteria on cell walls of Eragrostic tef. J. Agric. Sci. 95:313-323. crossref(new window)

19.
Munn, E. A., G. P. Hazlewood, and M. Graham. 1983. Uptake and incorporation of the products of proteolysis by the rumen bacterium Bacteroides ruminicola R8/4. Curr. Microbiol. 8:317-320. crossref(new window)

20.
Paunescu, E., A. Ciolac-Negoescu, and G. Pisica. 1964. The effect of Tween 80 and penicillin on the physicochemical properties of the cell wall in mycobacteria. Academie Republicii Populare Romine, Institutul de Biochimie, Studii si Cercetari de Biochimie. 7:83-89.

21.
Reese, E. T., and A. Maguire. 1969. Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiol. 17:242-245.

22.
Russell, J. B. 1983. Fermentation of peptides by Bacteroides ruminicola $B_14$. Appl. Environ. Microbiol. 45:1566-1574.

23.
Russell, J. B., and D. B. Wilson. 1988. Potential opportunities and problems for genetically altered rumen microorganisms. J. Nutr. 118:271-278.

24.
SAS. 1996. User's Guide: Statistics, Version 6 Editions. SAS Inst., Inc., Cary, NC. USA.

25.
Schewale, J. G., and J. C. Sadana. 1978. Cellulase and $\beta$-glucosidase production by basidiomycetes species. Can. J. Microbiol. 24:1204-1216.

26.
Scott, H. W., and B. A. Dehority. 1965. Vitamin requirements of several cellulolytic rumen bacteria. J. Bacteriol. 89:1169-1175.

27.
Theodorou, M. K., M. Gill, C. King-Spooner, and D. E. Beever. 1990. Enumeration of anaerobic chytridiomycetes as thallus forming units: a novel method for the quantitification of fibrolytic fungal populations from the digestive tract ecosystem. Appl. Environ. Micoriol. 56:1073-1078.

28.
Wallace, R. J., and M. L. Brammall. 1985. The role of different species of bacteria in the hydrolysis of protein in the rumen. J. Gen. Microbiol. 131:821-832.

29.
Weimer, P. J., J. M. Lopez-Guisa, and A. D. French. 1990. Effect of cellulose fine structure on kinetics of its digestion by mixed ruminal microflora. Appl. Environ. Microbiol. 56:2421-2429.

30.
Wildman, E. E., Jones, G. M., Wagner, P. E., Boman, R. L., Troutt Jr., H. F. and T. N. Lesch. 1982. A dairy cow body condition scoring system and its relationship to selected production characteristics. J. Dairy Sci. 65, pp. 495¯501.

31.
Wittenberger, C. L., A. J. Beaman, and L. N. Lee. 1978. Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius. J. Bacteriol. 133:231-239.

32.
Yazdi, M. T., J. R. Woodward, and A. Radford. 1990. The cellulase complex of Neurospora crassa:activity, stability and release. J. Gen. Microbiol. 136:1313-1319.