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Effect of Different Ratios of Concentrate and Roughage on Lipid Synthesis by Rumen Microorganisms In Vitro
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 Title & Authors
Effect of Different Ratios of Concentrate and Roughage on Lipid Synthesis by Rumen Microorganisms In Vitro
Sasaki, H.; Horiguchi, K.; Takahashi, T.;
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 Abstract
The effect of different feeding ratios of concentrate to roughage on ruminal lipid synthesis in vitro was examined. Three sheep fitted with a rumen fistula were fed three different ratios (8:2, 4:6 and 0:10) of concentrate and roughage, and their rumen liquor were used for incubation. -labeled glucose or sodium acetate as substrate was added to cultures of rumen liquor, and they were incubated for 6 h. The total lipid in the culture of the rumen liquor was extracted, and the percentage of excess was analyzed. The percentage of excess recovered when incubated with glucose increased with increased ratio of concentrate in the diet. The values of cultures incubated with glucose were higher than those incubated with sodium acetate except the roughage-only feeding. In the roughage-only diet, the percentage of excess when incubated with sodium acetate was highest of all diets. The recovery percentage of from glucose increased with increased ratio of concentrate. The recovery percentage of from sodium acetate addition in only roughage feeding was highest among the three diets. The recovery percentage of from glucose was markedly higher than that of sodium acetate addition in all feedings. The results indicate that high concentrate feeding facilitates lipid synthesis by rumen microorganisms, and that glucose may be the precursor for lipid synthesis rather than acetic acid.
 Keywords
Concentrate and Roughage;Ruminal Lipid Synthesis;Rumen Microorganism;
 Language
English
 Cited by
 References
1.
Abe, M. and T. Iriki. 1978. Effects of diet on the protozoa population in permeable continuous cultures of rumen contents. Br. J. Nutr. 39:255-264. crossref(new window)

2.
Abe, M., H. Shibui, T. Iriki and F. Kumeno. 1973. Relation between diet and protozoal population in the rumen. Br. J. Nutr. 29:197-202. crossref(new window)

3.
Dawson, R. M. C. and P. Kemp. 1967. The aminoethylphosphonate- containing lipids of rumen protozoa. Biochem. J. 105:837-842.

4.
Folch, J., M. Lees and G. H. Sloan-Stanley. 1967. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.

5.
Grubb, J. A. and B. A. Dehority. 1975. Effects of an abrupt change in ration from all roughage to high concentrate upon rumen microbial numbers in sheep. Appl. Microbiol. 30:404-412.

6.
Harfoot, C. G. 1981. Lipid metabolism in the rumen. In: Lipid Metabolism in Ruminant Animals (Ed. W. W. Christie). Oxford. Pergamon Press. pp. 21-55.

7.
Jenkins, T. C. 1993. Lipid metabolism in the rumen. J. Dairy Sci. 76:3851-3863.

8.
Jenkins, T. C. 1994. Regulation of lipid metabolism in the rumen. J. Nutr. 124:1372-1376.

9.
Latham, M. J., E. Sharpe and J. D. Sutton. 1971. The microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation. J. Appl. Bacteriol. 34:425-434.

10.
Morimoto, H. 1971. Doubutsueiyoushikenhou. Tokyo. Japan. Yokendo. pp. 448-450.

11.
Nakamura, K. 1985. Rumen senmouchu no seitai. In: Rumen No Sekai (Ed. M. Kandatsu and K. Suto). Tokyo. Japan. Nozan gyoson bunkakyokai. pp. 141-166.

12.
Patton, R. A., R. D. McCarthy and L. C. Jr. Griel, 1970. Lipid synthesis by rumen microorganisms. II. Further characterization of the effects of methionine. J. Dairy Sci. 53:460-465.

13.
Patton, R. A., R. D. McCarthy, L. G. Keske and L. C. Jr. Griel. 1968. Lipid synthesis by rumen microorganisms. I. Stimulation by methionine in vitro. J. Dairy Sci. 51:1310-1311.

14.
Polan, C. E., J. J. McNeil and S. B. Tove. 1964. Biohydrogenation of unsaturated fatty acids by rumen bacteria. J. Bacteriol. 88:1056-1063.

15.
Russell, J. B. and R. B. Hespell. 1981. Microbial rumen fermentation. J. Dairy Sci. 64:1153-1169.

16.
Sasaki, H., K. Horiguchi and T. Takahashi. 2000. Effects of the different ratios of concentrate and roughage on the concentration and composition of long chain fatty acids of rumen microorganisms and microorganisms-free-liquor in sheep. Anim. Sci. J. 71:J26-38.

17.
Sasaki, H., K. Horiguchi and T. Takahashi. 2001. Effects of different concentrate and roughage ratios on ruminal balance of long chain fatty acids in sheep. Asian-Aust. J. Anim. Sci. 14:960-965.

18.
Viviani, R. 1970. Metabolism of long-chain fatty acids in the rumen. Adv. Lipid Res. 8:267-346.

19.
Wallace, R. J. 1980. Cytoplasmic reserve polysaccharide of Selenomonas ruminantium. Appl. Environ. Microbiol. 39:630-634.

20.
Wu, Z., O. A. Ohajuruka and D. L. Palmquist. 1991. Ruminal synthesis, biohydrogenation and digestibility of fatty acids by dairy cows. J. Dairy Sci. 74:3025-3034.