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Effects of Surfactant Tween 80 on Enzymatic Accessibility and Degradation of Orchardgrass (Dactylis glomerata L.) at Different Growth Stages
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 Title & Authors
Effects of Surfactant Tween 80 on Enzymatic Accessibility and Degradation of Orchardgrass (Dactylis glomerata L.) at Different Growth Stages
Goto, M.; Bae, Hee-Dong; Yahaya, M.S.; Karita, S.; Wanjae, K.; Baah, J.; Sugawara, K.; Cheng, K.J.;
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The study evaluates the enzymatic dry matter (DM) degradability and water holding capacity of leaf and stem fractions of orchardgrass (Dactylis glomerata L.) at different growth stages with or without the presence of surfactant Tween 80. While Tween 80 significantly (p<0.05) increased water and enzyme holding capacities in the leaf blades fraction, less was observed in the fraction of leaf sheath and stem of orchardgrass. The enzyme holding capacity in the leaves was also altered more than that for water holding capacity. This resulted in the increased rate and extent of enzymatic hydrolysis of the leaf blade fractions at two growth stages, whereas little was with leaf sheath and stem fractions. It was also observed that at 0.005% concentrations of Tween 80 the enzymatic DM degradability of young leaf blades was higher (p<0.05) by 20-30% compared to that of the control, as well as for water and enzyme holding capacity. For matured leaf blades the DM degradability were increased with over 0.01% concentrations of the surfactant, but the increase was less than leaf blades of young orchardgrass. This result suggests the possibility of using the surfactant Tween 80 to improve forage digestibility in the rumen.
Tween 80;Enzymatic Degradability;Water and Enzyme Holding Capacities;
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Association of Official Analytical Chemists, 1990. Official methods of analysis. 14th ed. A.O.A.C. Washington, DC.

Akesenova, L. A., M. V. Dunaeva, E. A. Zak, Yu. F. Osipov and N. L. Klyachko. 1994. The effect of tween 80 and seed germination in winter wheat cultivars differing in drought rsistance Russian J. Plant Physiol. 41:557-559.

Castanon, M. and C. R. Wilke. 1981. Effects of the surfactant Tween 80 on enzymatic hydrolysis of newspaper. Biotechnol. Bioengin. Technol. 23:1365-1372. crossref(new window)

Gordon, A. H., J. A. Lomax, K. Dalgarno and A. Chesson. 1985. Preparation and composition of mes0phyll, epidermis and fiber cell walls from leaves of parenial ryegrass (Lolium perenne) and italian ryegrass (Lolium multiflorum) J. Sci. Food Agric. 36:509-519. crossref(new window)

Goto, M., T. Sato, O. Morita, K. Takabe and N. Inoue. 1993. Variations in anatomy and ultraviolet microspectrometry between normal and brawn midril mutant maizes possessing different rumen degradabilities. J. Sci. Food Agric. 63:427-434. crossref(new window)

Helle, S. S., S. J. B. Duff and D. G. Cooper. 1993. Effect of surfactants on cellulose hydrolysis. Biotechnol. Bioengin. 42:611-617. crossref(new window)

Linberg, J. E., I. E. Ternrud and O. Theander. 1984. Degradation of rare and chemical composition of different types of alkaline-treated straws during rumen digestion. J. Sci. Food Agric. 32:745-748.

Ooshima, H., M. Sakata and Y. Harano. 1986. Ehancement of enzymatic hydrolysis of cellulose by surfactant. Biotechnol. Bioengin. Technol. 28:1727-1734. crossref(new window)

Snedecor, G. W. and W. G. Cochran. 1980. Two-way classifications and analysis of variance In: Statistical Methods (Ed. G. W. Snedecor and W.G. Cochran7th).

Spackman, D. H., W. H. Stein and S. Moore. 1958. Chromatography of amino acids on sulfonated polystyrene resins. An improved system. Anal. Chem. 30:1185-1190. crossref(new window)

Steve, S. H., J. B. Duff Sheldom and G. C. David. 1993. Effect of surfactants on cellulose hydrolysis. Biotechnol. Bioengin. Technol. 42:611-617. crossref(new window)

Vadiveloo, J. 2000. Nutritional properties of the leaf and stem of rice straw. Anim. Feed Sci. Technol. 83:57-65. crossref(new window)

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Method for dietary fiber, neutral detergent fiber, neutral detergent lignin and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.

Wilson, J. R. 1993. Organization of forage plant tissues (Ed. H. G. Jung, D. R. Buxton, R. D. Hatfield and J. Ralph) In: Forage Cell Wall Structure and Digestibility. USA. pp. 1-32.