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Effects of Microbial Phytase Replacing Partial Inorganic Phosphorus Supplementation and Xylanase on the Growth Performance and Nutrient Digestibility in Broilers Fed Wheat-based Diets
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 Title & Authors
Effects of Microbial Phytase Replacing Partial Inorganic Phosphorus Supplementation and Xylanase on the Growth Performance and Nutrient Digestibility in Broilers Fed Wheat-based Diets
Peng, Y.L.; Guo, Y.M.; Yuan, J.M.;
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Two experiments were conducted with broilers to investigate the feasibility of microbial phytase replacing partial inorganic phosphorus supplementation and the synergistic effects of xylanase (320 FTU/kg) supplementation alone or in combination with phytase (750 U/kg) replacing 0.08% dietary inorganic phosphorus, on the growth performance and utilization of nutrients in broilers fed wheat-based diets. In Experiment 1, 540 broilers were fed five diets for 6 weeks. Diets C0 and C1 were corn-based diets and 0.08% inorganic P supplementation was replaced with 750 U phytase/kg feed in Diet C1. Diets W0, W1 and W2 were wheat-based diets supplemented with microbial phytase 0, 750, 750 U/kg feed and 0, 0.08% and 0.16% dietary inorganic P were replaced, respectively. In Experiment 2, 432 broilers were divided into four treatments to determine the synergistic effects of supplemental xylanase and phytase replacing 0.08% inorganic P. Four experimental diets were arranged according to a factorial design. The results indicated that addition of phytase increased the digestibility of phytic P by 31.0 to 55%, dramatically decreased the excretion of phytic P and total P by 31.6 to 55.0% and 13.8 to 32.9%, respectively (p<0.01). It is feasible to completely replace 0.08% inorganic phosphorus supplementation with microbial phytase 750 U/kg in corn- or wheat-based diets for broilers. Addition of xylanase alone or in combination with phytase replacing 0.08% dietary inorganic P, increased body weight gain and feed utilization efficiency of broilers fed wheat-based diets (p<0.10) and decreased overall mortality (p<0.10). In the groups of birds supplementing xylanase 320 FTU/kg feed, a marked elevation of the dietary AME was observed (p<0.05). Addition of phytase replacing 0.08% dietary inorganic phosphorus, concurrently with xylanase supplementation had additive effects on the apparent digestibility of dietary phytic P and overall feed conversion ratio (p<0.05).
Broilers;Phytase;Xylanase;Wheat-based Diet;Inorganic Phosphorus;
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아세아태평양축산학회지, 2009. vol.22. 6, pp.857-864 crossref(new window)
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아세아태평양축산학회지, 2010. vol.23. 9, pp.1198-1204 crossref(new window)
Significance of phytic acid and supplemental phytase in chicken nutrition: a review, World's Poultry Science Journal, 2008, 64, 04, 553  crossref(new windwow)
Alko Biotechnology. 1990. Assay of phytase activity, method B-021. Rajamaki, Finland.

Almirall, M., M. Frencesch, A. M. Perez-Vendrell, J. Brufau and E. Esteve_Garcia. 1995. The differences in intestinal viscosity produced by barley and $\beta$-glucanase alter digesta enzymes activities and ileal nutrient digestibilities more in broiler chicks than in cocks. J. Nutr. 125:947-955.

Annison, G. 1990. Polysaccharide composition of Australian wheats and the digestibility of their starches in broiler chicken diets. Aust. J. Exp. Agric. 30:183-186. crossref(new window)

Annison, G. 1993. The role of wheat non-starch polysaccharide in broiler nutrition. Aust. J. Agric. Res. 44:405-422.

AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Arlington, Virginia.

Bagheri, S. and L. Gueguen. 1985. Effect of wheat bran and pectin on the absorption and retention of phosphorus, calcium, magnesium and zinc by the growing pig. Rep. Nutr. Dev. 25:705-716. crossref(new window)

Barrier-Guillot, B., P. Casado, P. Maupetit, C. Jondreville and F. Gatel. 1996. Wheat phosphorus availability: 2. In vivo study in broilers and pigs: relationship with endogenous phytasic activity and phytic phosphorus content in wheat. J. Sci. Food Agric. 70:69-74. crossref(new window)

Bolton, W. 1955. The digestibility of the carbohydrate complex by birds of different ages. J. Agric. Sci. 46:420. crossref(new window)

Coelho, M. B. and E. T. Kornegay. 1996. Phytase in animal nutrition and waste management. BASF Corporation, Mount Olive, NJ.

Crouch, A. N., J. L. Grimes, P. R. Ferker and L. N. Thomas. 1997. Enzyme supplementation to enhance wheat utilization in starter diets for broilers and turkeys. J. Appl. Poult. Res. 6:147-154.

Eeckout, W. and M. De Paepe. 1994. Total phosphorus, phytatephosphorus and phytase activity in plant feedstuffs. Anim. Feed Sci. Technol. 47:19-29. crossref(new window)

Fengler, A. I. and R. R. Marquardt. 1988. Water-soluble pentosans from rye: Effects of rate of dialysis on the retention of nutrients by the chick. Cereal Chemistry 65:298-302.

Fruhback, G., R. Alonso, F. Marzo and S. Santidrian. 1995. A modified method for the indirect quantitative analysis of phytate in foodstuffs. Anal. Biochem. 225:206-212. crossref(new window)

Fuente, J. M., P. Perez De Ayala, A. Flores and M. J. Villamide. 1998. Effect of storage time on the metabolizable energy and digesta viscosity of barley-based diets for poultry. Poult. Sci. 77:90-97.

Iji, P. A., R. J. Hughes, M. Choct and D. R. Tivey. 2001. Intestinal structure and function of broiler chickens on wheat-based diets supplemented with a microbial enzyme. Asian-Aust. J. Anim. Sci. 14:54-60.

Jongbloed, A. W., Z. Mroz and P. A. Kemme. 1992. The effect of supplementary Aspergillus niger phytase in diets for pigs in concentration and apparent digestibility of dry matter, total phosphorus and phytic acid in different sections of the alimentary tract. J. Anim. Sci. 70:1155-1168.

Jongbloed, A. W., P. A. Kemme, Z. Mroz and R. Jongbloed. 1996. The effects of organic acids in diets for growing pigs on the efficacy of microbial phytase. In: Phytase in Animal Nutrition and Waste Management (Ed. M. B. Coelho and E. T. Kornegay). BASF Corp., Mount Olive, NJ. pp. 515-524.

Kiiskinen, T., J. Piironen and T. Hakonen. 1994. Effects of supplemental phytase on performance of broiler chickens. Agric. Sci. Fin. 3:457-466.

Kornegay, E. T., D. M. Denbow and Z. Zhang. 1997. Phytase supplementation of corn-soybean meal broilers diets from three to seven week. Poult. Sci. 76(Suppl. 1):6(Abstr.).

Kornegay, E. T. 1999. A review of phosphorus digestion and excretion as influenced by microbial phytase in poultry. BASF Technical symposium, January 16, Atlanta, GA. BASF Corp., Mount Olive, NJ.

Larsson, M., M. Minekus and R. Havenaar. 1997. Estimation of the bioavailability of iron and phosphorus in cereals using a dynamic in vitro gastrointestinal model. J. Sci. Food Agric. 74:99-106. crossref(new window)

Maisonnier, S., J. Gomez and B. Carre. 2001. Nutrient digestibility and intestinal viscosities in broiler chickens fed on wheat diets, as compared to maize diets with added guar gum. Br. Poult. Sci. 42:102-110. crossref(new window)

Marquardt, R. R., A. Brenes, Z. Zhang and D. Boros. 1996. The use of enzymes to improve nutrient availability in poultry feedstuffs. Anim. Feed Sci. Technol. 60:321-330. crossref(new window)

McCance, R. A. and E. M. Widdowson. 1944. Activity of the phytase in different cereal and its resistance to heat. Nature. 153:660. crossref(new window)

Mollah, Y., W. L. Bryden, I. R. Wallis, D. Balnave and E. F. Annison. 1983. Studies on low metabolizable energy wheats for poultry using conventional and rapid assay procedures and the effects of processing. Br. Poult. Sci. 24:81-89. crossref(new window)

Nelson, T. S. 1976. The hydrolysis of phytate phosphorus by chicks and laying hens. Poult. Sci. 55:1552-1562.

Newirk, R. W. and H. L. Classen. 1996. The effects of dietary xylanase, phytase and phosphorus on the performance of laying hens. Poult. Sci. 72(Suppl 1):17(Abstr.).

NRC. 1994. Nutrient requirements of poultry. 9th edn. National Academy Press, Washington, DC.

Ouhida, J. F., Perez J. GASA and F. Puchal. 2000. Enzymes ($\beta$-glucanase and arabinoxylanase) and/or sepiolite supplementation and the nutritive value of maize-barley-wheat based diets for broiler chickens. Br. Poult. Sci. 41:617-624. crossref(new window)

Peter, C. M. and David H. Baker. 2001. Microbial phytase does not improve protein-amino acid utilization in soybean meal fed to young chicks. J. Nutr. 131:1792-1797.

Piao, X. S., In K. Han, J. H. Kim, W. T. Cho, Y. H. Kim and Chao Liang. 1999. Effects of kemzyme, phytase and yeast supplementation on the growth performance and pollution reduction of broiler chicks. Asian-Aus. J. Anim. Sci. 12:36-41.

Qian, H., H. P. Veit, E. T. kornegay, V. Ravindran and D. M. Denbow. 1996. Effects of supplemental phytase and phosphorus on histological and other tibial bone characteristics and performances of broilers fed semi-purified diets. Poult. Sci. 75:618-626.

Rama Rao, S. V. and V. Ramasubba Reddy. 2001. Phytic phosphorus for eco-friendly products. Poult. International. 40:46-52.

Ravindran, V. and W. L. Bryden. 1997. Influence of dietary phytic acid and available phosphorus levels on the response of broilers to supplemental Natuphos. Poultry Research Foundation of the University of Sydney. Report to BASF Australia Ltd., Sydney, Australia.

Ravindran, V., L. I. Hew, G. Ravindran, R. J. Gill, P. H. Pittolo and W. L. Bryden. 1999. Influence of xylanase supplementation on the apparent metabolisable energy and ileal amino acid digestibility in a diet containing wheat and oats, and on the performance of three strains of broiler chickens. Aust. J. Agric. Res. 50:1159-1163. crossref(new window)

Rogel, A. M., E. F. Annison, W. L. Bryden and D. Balnave. 1987. The digestion of wheat starch in broiler chickens. Aust. J. Agric. Res. 38:639-649. crossref(new window)

Schoner, F. J. 1993. Comparative effects of phytase and inorganic phosphorus in male chicken: the influence on performance data, mineral retention and dietary Ca. J. Anim. Physiol. Anim. Nutr. 69:235-244. crossref(new window)

Scott, T. A., F. G. Silversides, H. L. Classen, M. L. Swift and M. R. Bedford. 1999. Prediction of the performance of broiler chicks from apparent metabolizable energy and protein digestibility values obtained using a broiler chick bioassay. Can. J. Anim. Sci. 79:59-64.

Simons, P. C. M. and H. A. J. Versteegh. 1990. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr. 64:525-540. crossref(new window)

Um, J. S., H. S. Lim, S. H. Ahn and I. K. Paik. 2000. Effects of microbial phytase supplementation to low phosphorus diets on the performance and utilization of nutrients in broiler chickens. Asian-Aus. J. Anim. Sci. 13:824-829.

Yinbo, Q., G. Peiji, W. Dong, Z. Xin and Z. Xiao. 1996. Production, Characterization, and application of cellulase-free xylanase from Aspergillus niger. Appl. Biochem. Biotechnol. 57/58: 375-381. crossref(new window)

Zhang, Z. B., E. T. Kornegay, J. S. Radcliffe, D. M. Denbow, H. P. Veit and C. T. Larsen. 2000. Comparison of genetically engineered microbial and plant phytase for young broilers. Poult. Sci. 79:709-717.

Zyla, Krzysztof, Dorota Gogol, Jerzy Koreleski, Sylwester Swiatkiewicz and David R Ledoux. 1999a. Simultaneous application of phytase and xylanase to broiler feeds based on wheat: in vitro measurements of phosphorus and pentose release from wheats and wheat-based feeds. J. Sci. Food Agric. 79:1832-1840. crossref(new window)

Zyla, Krzysztof, Dorota Gogol, Jerzy Koreleski, Sylwester Swiatkiewicz and David R. Ledoux. 1999b. Simultaneous application of phytase and xylanase to broiler feeds based on wheat: feeding experiment with growing broilers. J. Sci. Food Agric. 79:1841-1848. crossref(new window)