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Changes in Ovarian and Placental 20α-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat
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 Title & Authors
Changes in Ovarian and Placental 20α-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat
Seong, H.H.; Min, K.S.; Kang, M.H.; Yoon, J.T.; Jin, H.J.; Chung, H.J.; Chang, W.K.; Yun, S.G.; Shiota, Kunio;
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 Abstract
The enzyme -hydroxysteroid dehydrogenase (-HSD) catabolizes progesterone to -dihydroprogesterone (-OHP), and is appeared in rat corpora luteal and placenta. A polled samples of 10-15 placental and ovarian tissues collected from each individual rat were subjected to measurement of -HSD activity. A -HSD activity in the cytosol fraction was based on the generation of NADPH. In this study, it is designed to study cytosolic -HSD activity in rat ovarian and placenta during pregnancy, and its relationship to embryonic mortality. It was found that from days 5 to 18 of pregnancy the -HSD activities steady by decreased but at parturition time rapidly increased in ovary. On the other hand, placental cytosolic -HSD activities were high detected from days 8 to 10 of pregnancy, not detectable from days 11 to 20 of pregnancy, but again very high at the time of parturition. Analysis of DEAE column chromatography revealed that two different types of -HSD (HSD-1 and HSD-2) were found with similar activity in the placental cytosol on day 10 of pregnancy. The number of fetuses on day 10 of pregnancy was 15.4 and decreased significantly to 12.9 on day 12. The results suggested that expression of -HSD in the placental tissues seems to be related the number of fetal survived in the specific time (days 11 and 12) which spontaneous fetal loss occurs.
 Keywords
-HSD Activity;Progesterone;-dihydroprogesterone;Rat Ovary;
 Language
English
 Cited by
 References
1.
Albarracin, C. T., T. G. Parmer, W. R. Duan, S. E. Nelson and G. Gibori. 1994. Identification of a major prolactin-regulated protein as 20 alpha-hydroxysteroid dehydrogenase: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression. Endocrinology 134:2453-2460. crossref(new window)

2.
Bladford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities utilizing the principle of protein-dye binding. Annu. Biochem. 72:248-254. crossref(new window)

3.
Clarke, C. L and R. L. Sutherland. 1990. Progesterone regulation of cellular proliferation. Endocrine Rev. 14:266-301.

4.
Csapo, A. I and W. G. Wiest. 1969.An examination of the quantitative relationship between progesterone and the maintenance of pregnancy. Endocrinology 85; 735-746 crossref(new window)

5.
Fukuda, T., K. Hirato, T. Yanaihara and T. Nakayama. 1986. Microsonal $20{\alpha}$-atiol and $20{\alpha}$-hydroxysteroid dehydrogenase activity for progesterone in human placenta. Endocrinol Japon 33:361-368. crossref(new window)

6.
Kuhn, N. J and M. S. Briley. 1970. The roles of pregn- 5-ene-$3{\beta}$, $20{\alpha}$-atiol and $20{\alpha}$-hydroxysteroid dehydro-genase in the control of progesterone synthesis preceding parturition and lactogenesis in the rat. Biochemical J. 117:193-201.

7.
Matsuda, J., K. Noda, K. Shiota and M. Takahashi. 1990. Participation of ovarian $20{\alpha}$-hydroxysteroid dehydrogenase in luteotrophic and luteolytic processes during rat pseudopregnancy. J. Reprod. Fertil. 88: 467 - 478.

8.
Matsuyama, S., K. Shiota and M. Takahashi. 1990. Possible role of transforming growth factor-$\beta$ as a mediator of lucotropic action of prolactin in the rat luteal cell cultures. Endocrinology 127:1561-1567. crossref(new window)

9.
Miura, R., K. Shiota, K. Noda, S. Yagi, T. Ogawa and M. Tahahashi. 1994. Molecular cloning of cDNA for rat ovarian 20 alpha-hydroxysteroid dehydrogenase (HSD1). Biochem. J. 299:561-567.

10.
Mori, M. and W. G. Wiest. 1979. Purification of $20{\alpha}$- hydroxysteroid dehydro-genase by affinity chromatography. J. Steroid Biochem. 14:1443-1449.

11.
Murakami, N., M. Takahashi, Y. Suzuki and K. Homma. 1982. Responsiveness of despersed rat luteal cells to luteinizing hormone and PRL during the estrous cycle and early pseudopregnancy. Endocrinology 111:500:508.

12.
Nakajin, S., Y. Kawal, S. Ohno and M. Shinoda. 1989. Purification and characterization of pig adrenal $20{\alpha}$-hydroxysteroid dehydro-genage. J. Steroid Biochem. 33:1181-1198. crossref(new window)

13.
Nancarrow, C. D. M. A. Sharaf and F. Sweet. 1981. Purification of $20{\alpha}$ hydroxysteroid oxidoreductase from bovine fetal erythrocytes. Steroids 37:530-553. crossref(new window)

14.
Noda, K., H. H. Seong, K. Shiota and M. Takahashi. 1992. Changes of two kinds of $20{\alpha}$- hydroxysteroid dehydrogenase during luteal phase in the rat. J. Reprod. Develop. 38:49-53. crossref(new window)

15.
Noda, K., K. shiota and M. Takahashi. 1991. Purification and characterization of rat ovarian $20{\alpha}$-hydroxysteroid dehydrogenase. Biochem. Biophys. Acta. 1079:112-118.

16.
Olofsson. J., E. Norjavaara and G. Selstam. 1992. Synthesis of prostaglandin F2 alpha, E2 and prostacyclin in isolated corpora lutea of adult pseudopregnant rats throughout the luteal lifespan. Prostaglandins Leukot Essent Fatty Acids 46:151-161. crossref(new window)

17.
Pineda, J.A., M. F. Salinas and J. C. Warren. 1985. Purification and characterization of $20{\alpha}$-hydroxysteroid dehydro-genase from bull testis. J. Steroid Biochem. 23:1001-1006. crossref(new window)

18.
Pollard, J. W., A. Bartocci, R. Arceci, A. Orlofsky, M. B. Ladner and E. R. Stanley. 1987. Apparent role of the macrophage growth factor, CSF-1, in placental development. Nature 330:484-486. crossref(new window)

19.
Seong, H. H., K. Shiota, K. Noda, A. Ogure. T. Asano and M. Takahashi. 1992. Expression of activites of two $20{\alpha}$-hydroxysteroid dehydrogenase in rat corpora lutea. J. Reprod. Fertil. 96:573-580.

20.
Sharaf, M. and F. Sweet. 1982. Dual activity at an enzyme active site: $3{\beta}$, $20{\alpha}$- hydroxysteroid oxidoreductase from fetal blood. Biochemistry 21:4615-4620. crossref(new window)

21.
Shikita. M., H. Inano and B. Tamaoki. 1967. Further studies in $20{\alpha}$-hydroxysteroid dehydro genase of rat testes. Biochemistry 6:1760-1764. crossref(new window)

22.
Shiota, K., H. H. Seong, K. Noda, N. Hattori, A. Ikeda, A. Ogura, S-I. Itagaki, M. Takahashi and T. Ogawa. 1993. $20{\alpha}$-hydroxysteroid dehydrogenase activity in rat placenta. Endocrine J. 40:673-681. crossref(new window)

23.
Shiota, K., N. Sasaki, N. Hattori, R. Miura, T. Hasegawa, J-X. Guang, K. Noda and M. Takahashi. 1991. Cytosolic $20{\alpha}$-hydroxysteroid dehydrogenase activity in spontaneous Neoplasms in the dog and cat. J. Vet. Med. Sci. 53:549-552.