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Effects of Enzyme Application Method and Levels and Pre-treatment Times on Rumen Fermentation, Nutrient Degradation and Digestion in Goats and Steers
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 Title & Authors
Effects of Enzyme Application Method and Levels and Pre-treatment Times on Rumen Fermentation, Nutrient Degradation and Digestion in Goats and Steers
Hong, S.H.; Lee, B.K.; Choi, N.J.; Lee, Sang S.; Yun, S.G.; Ha, J.K.;
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Present study investigate the effect of enzyme supplementation, methods (applied to rumen or enzyme treated diet) compared with no enzyme diet, on rumen fermentation and apparent nutrient digestibility in a Latin square design with three rumen cannulated Korean Native goats. In situ rumen degradation kinetics was studied in three rumen cannulated Holstein steers. Three diets were, no enzyme, 1% enzyme in rumen and 1% enzyme in diet. The enzyme was sprayed onto forage, and the forage: concentrate ratio was 30:70. Degradation kinetics was studied with three enzyme levels (0, 1 and 2%, w/w) and four pre-treatment times (0, 1, 12 and 24 h). Results suggested that enzyme application method did not affect rumen fermentation, ruminal enzyme activity and total tract apparent digestibility. Nutrient degradation rate and effective degradability of DM, NDF and ADF increased with increasing enzyme level and pre-treatment times. Degradation of nutrients was affected by enzymes levels or pre-treatment times. Therefore, it is probable that the improved degradation may be due to the supplemented exogenous hydrolytic enzymes under a certain condition.
Enzyme;Digestibility;Rumen;Fermentation;In situ Degradation;
 Cited by
AOAC. 1990. Official Methods of Analysis. 15th Edition. Association of Official Analytical Chemists. Washington DC.

Beauchemin, K. A., L. M. Rode and V. J. H. Sewalt. 1995. Fibrolytic enzymes increase fibre digestibility and growth rate of steers fed dry forages. Can. J. Anim. Sci. 75:641-644.

Beauchemin, K. A. and L. M. Rode. 1996. Use of feed enzymes in ruminant nutrition. In: Animal Science Research and Development-Meeting Future Challenges (Ed. L. M. Rode). Minister of Supply and Services Canada, Ottawa, ON. pp. 104-159.

Beauchemin, K. A., S. D. M. Jones, L .M. Rode and V. J. H. Sewalt. 1997. Effects of fibrolytic enzyme in corn or barley diets on performance and carcass characteristics of feedlot cattle. Can. J. Anim. Sci. 77:645-653.

Beauchemin, K. A., W. Z. Yang and L. M. Rode. 1999. Effects of grain source and enzyme additive on site and extent of nutrient digestion in dairy cows. J. Dairy Sci. 82:378-390.

Beauchemin, K. A., D. P. Morgavi, T. A. McAllister., W. Z. Yang and L . M. Rode. 2001. The use of enzymes in ruminant diets. In: Recent Advances in Animal Nutrition (Ed. P. C. Garnsworthy and J. Wiseman). Nottingham University Press. Nottingham. pp. 298-322.

Chancy, A. L. and E. P. Marbach. 1962. Modification reagents for determination of urea and ammonia. Clin. Chem. 8:130.

Chen, K. H., J. T. Huber., J. Simas, C. B. Theurer, P. Yu, S. C. Chan., F. Santos, Z. Wu, R. S. Swingle and E. J. DePeters. 1995. Effect of enzyme treatment or steam-flaking of sorghum grain on lactation and digestion in dairy cows. J. Dairy Sci. 78:1721-1727.

Erwin, E. S., G. J. Marco and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 44:1768-1770.

Feng, P., C. W. Hunt, G. T. Pritchard and W. E. Julien. 1996. Effect of enzyme preparations on in situ and in vitro degradation and in vivo digestive characteristics of mature cool-season grass forage in beef steers. J. Anim. Sci.. 74:1349-1357.

Gashe, B. A. 1992. Cellulase production and activity by Trichoderma sp. A-001. J. Appl. Bact. 73:79-82.

Groleau, D. and C. W. Forsberg. 1981. Cellulolytic activity of the rumen bacterium Bacteroides succinogenes. Can. J. Microbiol. 27:517-530.

Hristov, A. N, L. M. Rode, K. A. Beauchemin and R. L. Wuerfel. 1996. Effect of a commercial enzyme preparation on barley silage in vitro and in sacco dry matter degradability. Proceedings, Western Section, American Society of Animal Science, Rapid City, South Dakota, Vol. 47:282-284.

Hristov, A. N., T. A. McAllister and K-J. Cheng. 1998. Effect of dietary or abomasal supplementation of exogenous polysaccharide-degrading enzymes on rumen fermentation and nutrient digestibility. J. Anim. Sci. 76:3146-3156.

Krause, M., K. A. Beauchemin, L. M. Rode, B. I. Farr and P. Norgaard. 1998. Fibrolytic enzyme treatment of barley grain and source of forage in high grain diets fed to growing cattle. J. Anim. Sci. 96:1010-1015.

Kung, L., Jr., R. S. Tung, K. G. Maciorowske, K. Buffem and K. Knutsen. 1991. Effecs of plant cell-wall-degrading enzymes and latic acid bacteria on silage fermentation and composition J. Dairy Sci. 74:4284-4296.

Lewis, G. E., C. W. Hunt, W. K. Sanchez, R. Treacher, G. T. Pritchard and P. Feng. 1996. Effect of direct-fed fibrolytic enzymes on the digestive characteristics of a forage-based diet fed to beef steers. J. Anim. Sci. 74:3020-3028.

McAllister, T. A., S. J. Oosting, J. D. Popp, Z. Mir, L. J. Yank, A. N. Hristov, R. J. Treacher and K-J. Cheng. 1999. Effect of exogenous enzymes on digestibility of barley silage and growth performance of feedlot cattle. Can. J. Anim. Sci. 79:353-360.

Morgavi, D. P., K. A. Beauchemin, V. L. Nsereko, L. M. Rode, T. A. McAllister, A. D. Iwaasa, Y. Wang and W. Z. Yang. 2001. Resistance of feed enzymes to proteolytic inactivation by rumen microorganisms and gastrointestinal proteases. J. Anim. Sci. 79:1621-1630.

Nsereko, V. L., D. P. Morgavi, K. A. Beauchemin and L. M. Rode. activities by extracts from silages. Can. J. Anim. Sci. 80:523-526.

Nussio, L. G., J. T. Huber, C. B. Theurer, C. B. Nussio, J. Santos, M. Tarazon, R. O. Lima-Filho, B. Riggs, M. Lamoreaux and R. J. Treacher. 1997. Influence of a cellulase/xylanase complex(C/X) on lactational performance of dairy cows fed alfalfa hay based diets. J. Dairy Sci. 80(Suppl. 1):220(Abstr.)

Orskove, E. R. and I. McDonal. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92:499-503. crossref(new window)

Ridla, M. and S. Uchida. 1999. Comparative study on the effects of combined treatments of lactic acid bacteria and cellulases on the cell wall compositions and the digestibility of Rhodesgrass (Chloris gayana Kunth.) and Italian Ryegrass (Lolium multiflorum Lam.) silages. Asian-Aust. J. Anim. Sci. 12(4):531-536.

Rode, L. M., W. Z.Yang and K. A. Beauchemin. 1999. Fibrolytic enzyme supplements for dairy cows in early lactation. J. Dairy Sci. 82:2121-2126.

Rust, J. W., N. L. Jacobson, A. D. McGillard and D. K. Hotchkiss. 1965. Supplementation of dairy calf diets with enzymes. II. Effect on nutrient utilization and on composition of rumen fluid. J. Anim. Sci. 24:156-160.

SAS. 1990. SAS Users Guide, Version 8, SAS Institute Inc., Cary, NC.

Stokes, M. R. and S. Zheng. 1995. The use of carbohydrase enzymes as feed additives for early lactation cows. Proceedings of the 23rd Conference on Rumen Function, Chicago, Illinois, p. 34(Abstr.)

Treacher, R., T. A. McAllister, J. D. Popp, Z. Mir, P. Mir and K-J. Chen. 1997. Effect of exogenous cellulases and xylanases on feed utilization and growth performance of feedlot steers. Can. J. Anim. Sci. 77:541(Abstr.)

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods of dietary fibre neutral detergent fibre and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.

Wallace, R. J., S. J. A. Wallace, N. McKain, V. L. Nsereko and G. F. Hartnell. 2001. Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal microorganisms in vitro. J. Anim. Sci. 79:1905-1916.

Wang, Y., T. A. McAllister, L. M. Rode, K. A. Beauchemin, D. P. Morgavi, V. L. Nsereko, A. D. Iwaasa and W. Yang. 2001. Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the Rumen Simulation Technique (Rusitec). Br. J. Nutr. 85:325-332. crossref(new window)

Yang, W. Z., K. A. Beauchemin and L. M. Rode. 1999. Effects of enzyme feed additives on extent of digestion and milk production of lactating dairy cows. J. Dairy Sci. 82:391-403.

Yang, W. Z., K. A. Beauchemin and L. M. Rode. 2000. A comparison of methods of adding fibrolytic enzymes to lactating cow diets. J. Dairy Sci. 83:2512-2520.