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Isotyping of Immunoglobulin G Responses of Ruminants and Mice to Live and Inactivated Antigens of Cowdria ruminantium the Causative Agent of Cowdriosis in Ruminants
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 Title & Authors
Isotyping of Immunoglobulin G Responses of Ruminants and Mice to Live and Inactivated Antigens of Cowdria ruminantium the Causative Agent of Cowdriosis in Ruminants
Kibor, A.C.; Sumption, K.J.; Paxton, E.A.;
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 Abstract
The Immunoglobulin and isotype immune responses of domestic ruminants and mice to Cowdria. ruminantium live infection or by immunization with inactivated organisms were determined by the enzyme linked immunosorbent assay and Western blotting. Immunization of goats with inactivated elementary bodies (IEBs) led to a predominant isotype response. This indicated that a Th2 response was induced. After challenge, the IgG isotype responses were mixed whereby both and antibodies were detected. Two goats that survived virulent challenge had a predominant isotype response. In cattle live infection by natur l challenge or experiment led to a predominant isotype response. Immunization of cattle with IEBs however led to mixed IgG responses characterized by similar and ratios. In the mouse live infection led to a predominant isotype response. This indicated the mouse developed a true Th1 type cell mediated immune response when inoculated with live organisms. Immunization with inactivated organisms on the other hand led to a dominant response. It is evident from this work that the immune responses of ruminants and mice to C. ruminantium are different and that using mice as the experimental model for immune responses to Cowdria ruminantium. is not the appropriate.
 Keywords
Immunoglobulin G-isotypes;Ruminants;Mice;Cwdria Ruminantium,;Antigens;
 Language
English
 Cited by
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