Advanced SearchSearch Tips
Isotyping of Immunoglobulin G Responses of Ruminants and Mice to Live and Inactivated Antigens of Cowdria ruminantium the Causative Agent of Cowdriosis in Ruminants
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Isotyping of Immunoglobulin G Responses of Ruminants and Mice to Live and Inactivated Antigens of Cowdria ruminantium the Causative Agent of Cowdriosis in Ruminants
Kibor, A.C.; Sumption, K.J.; Paxton, E.A.;
  PDF(new window)
The Immunoglobulin and isotype immune responses of domestic ruminants and mice to Cowdria. ruminantium live infection or by immunization with inactivated organisms were determined by the enzyme linked immunosorbent assay and Western blotting. Immunization of goats with inactivated elementary bodies (IEBs) led to a predominant isotype response. This indicated that a Th2 response was induced. After challenge, the IgG isotype responses were mixed whereby both and antibodies were detected. Two goats that survived virulent challenge had a predominant isotype response. In cattle live infection by natur l challenge or experiment led to a predominant isotype response. Immunization of cattle with IEBs however led to mixed IgG responses characterized by similar and ratios. In the mouse live infection led to a predominant isotype response. This indicated the mouse developed a true Th1 type cell mediated immune response when inoculated with live organisms. Immunization with inactivated organisms on the other hand led to a dominant response. It is evident from this work that the immune responses of ruminants and mice to C. ruminantium are different and that using mice as the experimental model for immune responses to Cowdria ruminantium. is not the appropriate.
Immunoglobulin G-isotypes;Ruminants;Mice;Cwdria Ruminantium,;Antigens;
 Cited by
Bird, P., H. T. Reyburn, B. A. Blacklaws, D. Allen, P. Nettleton, D. L. Yirrel, N. Watt, D. Sargan and McConell. 1995. The restricted $IgG_1$ antibody response to Maedi-Visna virus following infection but not immunization with recombinant gag protein. Clin Expt. Immunol., 102:274-280.

Byrom, B., S. P. Mahan and A. F. Barbet. 1993. The development of antibody to Cowdria ruminantium in mice and its role in heartwater disease. Revue d'Elev. Med. Vet des Pays Trop. 46:197-201.

Camus, E., Barre, D. Martinez, N. and G. Uilenberg. 1996. Heartwater: A Review, OIE Paris, 1996.

Du Plessis, J. L. and L. Malan. 1987. The application of indirect fluorescent antibody test in research on heartwater. Onderst. J. Vet. Res. 54:319-325.

Du Plessis, J. L., P. Berche and L. Van Gas. 1991. T cell mediated immunity to Cowdria ruminantium in mice the protective role of $Lyt2^+$ T cells. Onderst. J. Vet. Res. 58:171-179.

Du Plessis, J. L. 1993. An in vitro test to demonstrate the inhibitory effect of homologous immune serum on infectivity of Cowdria ruminantium. Onderst. J. Vet. Res. 60:69-73.

Du Plessis, J. L., J. D. Bezuidenhout, M. S. Brett, E. Camus, F. Jongejan, S. M. Mahan and D. Martinez. 1993. The serodiagnosis of heartwater: a comparison of five tests. Rev Elev. Med Vet Pays Trop. 46:123-129.

Estes, D. M., A. Hirano, V. T. Heussler, D. A. E. Dobbelaere and W. C. Brown. 1995. Expression and biological activities of bovine Interleukin 4: Effects of recombinant bovine interleukin 4 on T cell proliferation and B cell differentiation and proliferation in vitro. Cell. Immunol. 163:268-273. crossref(new window)

Jongejan, F., M. J. C. Thielemans, M. De Groot, P. J. S. Van Kooten and B. A. M. van Zeijst. 1991. Competetive enzyme linked immunosorbent assay for heartwater disease using monoclonal antibodies against Cowdria ruminantium specific 32 kilodalton protein. Vet. Microbiol, 28:119-121. crossref(new window)

Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the Bacteriophage T4. Nature 227:680-686. crossref(new window)

Lally, N. C., S. Nicol, E. A. Paxton, C. M. Cary and K. J. Sumption. 1995. The Cowdria rumunantium GroEL operon Microbiology 141:2091-2100.

Lawrence, J. A., J. Malika, A. P. Whiteland and P. T. Kafuwa. 1995. Reactions of heartwater vaccination in crossbred Zebu cattle. Onderst. J. Vet. Res. 62:19-29.

Mahan, S. M., N. Tebele, D. Mkwedeya, S. Semu, C. B. Nyathi, L. A. Wassink, P. J. Kelly, T. Petre and A. F. Barbett. 1993. An immunoblotting diagnostic assay for heartwater based on the immunodominant 32 kilodalton protein of Cowdria ruminantium detects false positives in field sera. J. Clin. Microbiol, 31:2729-2737.

Martinez, D., C. Sheikboudou, P. O. Couraud and A. Bensaid. 1993. Protection of goats against heartwater is acquired by immunisation with inactivated elementary bodies of Cowdria ruminantium. Rev. Elev. Med. Vet. Trop. 46:229.

McQuire, T. C., A. J. Musoke and T. Kurth. 1979. Functional properties of bovine $IgG_1$ and $IgG_2$: interaction with complement, macrophages neutrophils and skin. Immunology 38:249-256.

Mossman, T. R. and R. L. Coffman. 1989. TH1 and TH2 cells: Different patterns of lymphokine secretion lead to different functional properties. Ann. Rev. Immunol. 7:145-73. crossref(new window)

Neitz, A. W. H., G. J. Wiljoen, J. D. Bezuidenhout, P. T. Oberem, L. Visser and N. M. J. Vermuelen. 1986. Detection of Cowdria ruminantium antigens during the course of heartwater disease by means of an enzyme linked immunosorbent assay. Onderst. J.Vet. Res 53:205-207.

Pow, E., E. A. Paxton and K. J. Sumption. 1993. Culture in vitro of Cowdria ruminantium in bovine endothelial cells Trans. Royal Soc. Trop. Med. Hgy. 8723

Rossouw, M., A. W. H. Neitz, D. T. De Waal, J. L. Du Plessis, L. Van Gas and S. Brett. 1990. Identication of antigenic proteins of Cowdria ruminantium. Onderst. J. Vet. Res. 57:215-221.

Semu, S. M., S. Mahan, C. E. Yunker and M. J. Burridge. 1992. Development and persistence of Cowdria antibodies following experimental infection detected by the indirect fluorescent antibody test. Vet. Immunol Immunopathol 33:339-352. crossref(new window)

Soldan, A. W., T. L. Norman, S. Masaka, E. A. Paxton, R. M. Edelsten and K. J. Sumption. 1993. Seroconversion to Cowdria ruminantium of Malawi zebu calves reared under different tick control strategies. Rev.Elev. Med. Vet. Trop., 46:171-177.

Towbin, H., T. Staehelin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and someapplications Proc. Nat Acad Sci. USA 76:4350-4354 crossref(new window)

Uilenberg, G. 1983. Heartwater (Cowdria ruminantium infection) Current Status). Advan Vet Sci Comp Med. 27:427-480.