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Characterization of Korean Cattle Keratin IV Gene
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 Title & Authors
Characterization of Korean Cattle Keratin IV Gene
Kim, D.Y.; Yu, S.L.; Sang, B.C.; Yu, D.Y.;
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Keratins, the constituents of epithelial intermediate filaments, are precisely regulated in a tissue and development specific manner. There are two types of keratin in bovine. The type I is acidic keratin and the type II is neutral/basic keratin. 1.5 kb of 5' flanking sequence of Korean cattle Keratin IV gene, type II keratin (59 kDa), was cloned and sequenced. A symmetrical motif AApuCCAAA are located in a defined region upstream of the TATA box. Proximal SP1, AP1, E-box and CACC elements as the major determinants of transcription are identified. When it was compared to the bovine sequence from -600 bp to ATG upstream, the homology was 97% in nucleotide sequence. Several A and T sequences, located in the promoter region, are deleted in the Korean cattle. An expression vector consisted of Korean cattle Keratin IV gene promoter/SV40 large T antigen was transfected to HaCaT cell (Epithelial keratinocyte). The transformed HaCaT cells showed active proliferation when treated with PDGF (Platelet-derived growth factor) in 0.3% soft agar compared to control cells. These results indicate that Korean cattle Keratin IVgene promoter can be used as a promoter for transfection into epithelial cell.
Keratinocyte;PDGF;Keratin IV Gene;Korean Cattle;
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Alpana, G., T. Malati and P. D. Gupat. 1997. Differential reduction in the expression of keratin polypeptide in human gastric carcinomas. Cancer. Dete. Prev. 21(2):129-134.

Angel, R., V. Miguel, B. Ana, L. Fernando and L. J. Jose. 1995. A 5'-upstream region of a bovine keratin 6 gene confers tissuespecific expression and hyperliferation-related induction in transgenic mice. Proc. Natl. Acad. Sci. USA. 92:4783-4787. crossref(new window)

Antonello, R., S. I. Jang, C. Roberta, M. S. Peter and G. M. Nedialka. 1998. Effect of AP1 Transcription factors on the regulation of transcription in normal human epidermal Keratinocytes. J. Invest. Dermatol. 110:34-40. crossref(new window)

Eitan, S., S. Martin, P. Fabrice, B. Michelle and F. W. Erwin. 2000. The Mammalian UV respose: c-Jun induction is required for exit from p53-imposed growth arrest. Cell 103:897-907. crossref(new window)

Federio, C. M., H. Q. Mirian, M. M. Meytha and K. H. Walid. 1997. Cultivation, serial transfer, and differentiation of epidermal keratinocytes in serum-free medium. Biochem. Biophys. Res. Commun. 236:167-172.. crossref(new window)

Jiang, C. K., H. S. Epstein, M. Tomic, I. M. Freedberg and M. Blumenberg. 1989. Epithelial-specific keratin gene expression: identification of a 300 base-pair controlling segment. Nuc. Acids. Res. 18(2):247-253.

Jone, T., E. Thomas, S. Georg, S. Axel et al. 2000. A transgenic mouse line that develops early-onset invasive gastric carcinoma provides a model for carcinoembryonic antigentargeted tumor therapy. Int. Journal of Cancer. 86:863-869. crossref(new window)

Manfred, B., Z. Hanswalter and L. J. Jose. 1987. Differentially expressed bovine cytokeratin genes. Analysis of gene linkage and evolutionary conservation of 5'-upstream sequences. Embo. J. 6(3):567-575.

Manfred, B., L. J. Jose and W. F. Werner. 1989. Enhancer elements directing cell-type-specific expression of cytokeratin genes and changes of the epithelial cytoskeleton by transfections of hybrid cytokeratin genes. Embo. J. 8(1):117-126.

Oliver, G. O., D. J. Timothy and K. R. Anil. 1998. Transcription regulation of the differentiation-linked human K4 promoter is dependent upon esophageal-specific nuclear factors. J. Biol. Chem. 273(37):23912-23921. crossref(new window)

Park, W. S., R. R. Oh, J. Y. Park, J. H. Lee, M. S. Shin, H. S. Kim, H. K. Lee, Y. S. Kim, S. Y. Kim, S. H. Lee, N. J. Yoo and J. Y. Lee. 2000. Somatic mutations of the trefoil factor family 1 gene in gastric cancer. Gastroenterology 119:691-698. crossref(new window)

Peter, F. S., P. T. David, B. F. Karen and M. T. Jonathon. 1994. Stomach cancer in transgenic mice expressing human papillomaviurs type 16 early region genes from a keratin promoter. J. Gen. Virol. 75:1125-1137. crossref(new window)

Sanger F., S. Nicklen and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467. crossref(new window)

Seth, L. N., E. Winfried and D. J. Timothy. 1998. Mouse keratin 4 is necessary for internal epithelial integrity. J. Biol. Chem. 273(37):23904-23911. crossref(new window)

Tinsley, J. M., C. Fisher and P. F. Searle. 1992. Abnormalities of epidermal differentiation associated with expression of the human papillomavirus type 1 early region in transgenic mice. J. Gen. Virol. 73:1251-1260. crossref(new window)

Tomohiko, A., N. Shinzaburo, I. Osamu et al. 1997. Detection of pancreatic and gastric cancer cells in peripheral and portal blood by amplification of keratin 19 mRNA with reverse transcriptase-polymerase chain reaction. Journal of Cancer 72:408-411.

Yeo, J. S., J. W. Kim, T. K. Chang, D. H. Nam, J. H. Han and C. B. Choi. 2002. Detection of DNA Fragment to Differentiate Korean Cattle. Asian-Aust. J. Anim. Sci. 15(8):1071-1075.