Advanced SearchSearch Tips
Effects of Dietary Polyunsaturated Fatty Acids on Antibody Production and Lymphocyte Proliferation of Laying Hens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Dietary Polyunsaturated Fatty Acids on Antibody Production and Lymphocyte Proliferation of Laying Hens
Xia, Z.G.; Guo, Y.M.; Chen, S.Y.; Yuan, J.M.;
  PDF(new window)
The purpose of present study was to assess the effect of polyunsaturated fatty acids (PUFA) on the immune responses of laying hens. Three hundred and sixty hens at the age of 60 weeks were randomly assigned to ten diets, which contained no oil (CK), 1%, 3%, 5% fish oil (FO); 2%, 4%, 6% linseed oil (LO) and 2%, 4%, 6% corn oil (CO). After 5 weeks of feeding experimental diets, humoral and cellular immune responses were assayed. Laying hens were injected with Sheep Red Blood Cell (SRBC) and Bovine Serum Albumin (BSA) and antibody titers, which were measured on d6, d10, d14 after primary challenge and on d5, d9, d13 after secondary challenge. Concanavalin (ConA) and lipopolysaccharide (LPS) -stimulated proliferation of peripheral blood and spleen lymphocytes were assessed by [H] thymidine incorporation at the week age of 5 and 10, respectively. The results showed that antibody titers in FO-fed and LO-fed laying hens were higher than that in laying hens fed CO. The proliferation response to ConA was lower in laying hens that fed oils rich in n-3 fatty acids than that in laying hens fed CO. Higher level n-3 fatty acids can improve immune functions of laying hens. In conclusion, dietary fat source and level had a significant impact on immune responses of laying hens.
Laying Hens;Dietary Polyunsaturated Fatty Acids;Humoral Immunity;Cell-mediated Immunity;
 Cited by
Alder, H. E. and A. J. DaMassa. 1978. Toxicity of endotoxins to chicks. Avian Dis. 23:174-178. crossref(new window)

Alexander, N. J. and N. L. Smythe. 1988. Dietary modulation of in vitro lymphocyte function. Ann. Nutr. Metab. 32:192-199. crossref(new window)

Cherian, G. and J. S. Sim. 1992. Preferential accumulation of n-3 fatty acids in the brain of chicks from eggs enriched with n-3 fatty acids. Poult. Sci. 71:1658-1668.

Das, U. N. 1994. Beneficial effect of eicosapentaenoic and docosahexaenoic acids in the management of systemic lupus erythematosus and its relationship to the cytokine network. Prostaglandins Leukot. Essent. Fatty Acids 51:207-213. crossref(new window)

Friedman, A. and D. Sklan, 1995. Effect of dietary fatty acids on antibody production and fatty acid composition of lymphoid organs in broiler chicks. Poult. Sci. 74:1463-1469.

Fristche, K. L. and P. V. Johnston. 1989. Modulation of eicosanoid production and cell-mediated cytotixicity by dietary $\alpha$–linolenic acid in BABL/c mice. Lipids 24:305-311. crossref(new window)

Fristche, K. L. and P. V. Johnston. 1990. Effects of dietary omega-3 fatty acids on cell-mediated cytotoxic activity in BALB/c mice. Nutr. Res. 10:577-588. crossref(new window)

Fristche, K. L., N. A. Cassity and S. C. Huang. 1991. Effect of dietary fat source on antibody production and lymphocyte proliferation in chickens. Poult. Sci. 70:611-617.

Hovi, T., J. Suni, L. Hortling and A. Vaheri, 1978. Stimulation of chicken lymphocyte by T- and H-cell mitogens. Cell. Immunol. 39:70-78. crossref(new window)

Hudson, L. and F. C. Hay. 1976. Haemagglutination. Pages 125-130 in: Practical Immunology. Blackwell Scientific. Publications, London, England.

N. M. Jeffery, P. Sanderson, E. A. Newsholme and P. C. Calder. 1996. Effects of varying the omega-6:omega-3 ratio of the diet upon immune function in the rat. Biochem. Soc. Trans. 24:77(Abstr.).

Johnston, P. V. 1985. Dietary fat eicosanoids and immunity. Adv. Lipids Res. 21:37-86.

Kelly, D. S., G. J. Nelson, C. M. Serrato, P. C. Schmidt and L. B. Branch. 1988. Effects of type of dietary fat on indices of immune status of rabbits. J. Nutr. 118:1367-1384.

Kollmorgen, G. M., W. A. Sansing, A. A. Lehman, G. Fischer, R. E. Longley, S. S. Alexander, Jr. M. M. King and P. B. McCay. 1979. Inhibition of lymphocyte function in rats fed high-fat diets. Cancer Res. 39:3458-3462.

Korver, D. R. and K. C. Klasing. 1997. Dietary fish oil alters specific and inflammatory immune responses in chicks. J. Nutr. 127:2039-2046.

Marshall, L. A. and P. V. Johnston. 1985. The influence of dietary essential fatty acids on rat immunocompetent cell prostaglandin synthesis and mitogen-induced blastogensis. J. Nutr. 115:1572-1580.

Meydani, S. N., A. H. Licchtenstein, S. Cornwall, M. Meydani, B. R. Goldin, H. Rasmussen, C. A. Dinarello and E. J. Schaefer. 1993. Immunologic effects of national cholesterol eduction panel step-2 diets without fish-diet n-3 fatty acid enrichment. J. Clin. Invest. 92:105-113. crossref(new window)

Mutia, R. and S. Uchida. 1999. Effects of feeding winged bean oil on cholesterol and lipid contents in egg and liver, and fatty acid composition of egg in Japanese Quail. Asian-Aus. J. Anim. Sci. Vol. 12:376-380.

National Research Council. 1994. Nutrient Requirements of Poultry, 9th rev. ed. National Academy Press, Washington, D.C.

Parmentier, H. K., M. G. B. Nieuwland, M. W. Barwegen, R. P. Kwkkel and J. W. Schrama. 1997. Dietary unsaturated fatty acids affect antibody responses and growth of chicken divergently selected for humoral responses to sheep red blood cells. Poult. Sci. 76:1164-1171.

Phetteplace, H. W., B. A. Watkins and P. B. Siegel. 1989. Effect of dietary n-6 and n-3 fatty acids on lipid metabolism in two genetic lines of broilers. Poult. Sci. 68(Suppl. 1):114(Abstr.).

Pricket, J. D., D. E. Trentham and D. R. Robinson. 1984. Dietary fish oil augments the induction of arthritis in rat immunized with type II collagen. J. Immunol. 132:725-729.

Prickett, J. D., D. R. Robinson and K. J. Bloch. 1982. Enhanced production of IgE and IgG antibodies associated with a diet enriched in eicosapentaenoic acid. Immunology 46:819-826.

Sijben, J. W. C., M. G. B. Nieuwland, B. Kemp, H. K. Parmentier and J. W. Schrama. 2001. Interactions and antiogen dependence of dietary n-3 and n-6 polyunsaturated fatty acids on antibody and responsiveness in growing layer hens. Poult. Sci. 80:885-893.

Toivanen, P. and A. Toivanen. 1973. Selective activation of chicken T lymphocyte by concanvalin A. J. Immunol. 111:1602-1603.

Vainio, O. and M. J. H. Ratcliffe. 1984. Proliferation of chicken peripheral blood leukocytes in response to pokeweed mitogen is macrophage dependent. Cell. Immunol. 85:235-243. crossref(new window)

Wang, Y. W., C. J. Field and J. S. Sim. 2000. Dietary polyunsaturated fatty acids alter lymphocyte subset proportion and proliferation, serum immunoglobulin G concentration, and immune tissue development in chicks. Poult. Sci. 79:1741-1748.

Yaqoob, P. and P. C. Calder. 1995. The effects of dietary manipulation on the production of murine T-cell-derived cytokines. Cytokine 7:548-553. crossref(new window)

Yaqoob, P., E. A. Newsholme, and P. C. Calder. 1994. The effect of dietary lipid manipulation on rat lymphocyte subsets and proliferation. Immunology 82:165-170.

Yoshino, S. and E. F. Ellis. 1989. Stimulation of anaphylaxis in the mouse footpad by dietary fish oil fatty acids. Prostaglandins Leukot. Essent. Fatty Acids 36:165-170. crossref(new window)