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Peptic Hydrolysate of Porcine Crude Myosin Has Many Active Fractions Inhibiting Angiotensin I-converting Enzyme
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 Title & Authors
Peptic Hydrolysate of Porcine Crude Myosin Has Many Active Fractions Inhibiting Angiotensin I-converting Enzyme
Katayama, Kazunori; Fuchu, Hidetaka; Sugiyama, Masaaki; Kawahara, Satoshi; Yamauchi, Kiyoshi; Kawamura, Yukio; Muguruma, Michio;
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 Abstract
In order to clarify one of the biological functions of pork, we investigated whether a peptic hydrolysate of denatured porcine crude myosin showed inhibitory activity against angiotensin I-converting enzyme (ACE), which contributed to hypertension. Our results indicated that this hydrolysate showed relatively strong activity, and we therefore attempted to separate the involved peptides, which were considered to be active substances. To isolate these active peptides, the hydrolysate was separated using a solidphase separation, gel filtration high-performance liquid chromatography (HPLC), and two kinds of reverse phase HPLC. In each stage of separation, many fractions were detected, almost all of which showed ACE inhibitory activity. Thus, we suggested that the activity of the hydrolysate as a whole was a result of the activities of the many individual peptides. Six peaks were distinguished, with yields from 34 to 596 ppm of original crude myosin. In addition to the six peaks, many other active fractions were found throughout the separation steps, strongly suggesting that whole porcine crude myosin itself had ACE inhibitory activity. Moreover, pork as food was considered to function as an ACE inhibitory material in vivo, because pork proteins consist primarily of crude myosin, which included almost all the myofibrillar structural proteins.
 Keywords
Angiotensin I-converting Enzyme;Peptide;Porcine Crude Myosin;Protease Digestion;Pepsin;
 Language
English
 Cited by
1.
Attenuating Development of Cardiovascular Hypertrophy with Hydrolysate of Chicken Leg Bone Protein in Spontaneously Hypertensive Rats,;;;;;;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 5, pp.732-737 crossref(new window)
2.
분리대두단백질 가수분해물의 Angiotensin-I Converting Enzyme 저해효과,백수연;도정룡;도건표;김현구;

한국식품영양과학회지, 2010. vol.39. 1, pp.8-13 crossref(new window)
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