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Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line
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 Title & Authors
Establishment and Identification of a Debao Pony Ear Marginal Tissue Fibroblast Cell Line
Zhou, X.M.; Ma, Y.H.; Guan, W.J.; Zhao, D.M.;
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The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was uccessfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.
Debao Pony;Ear Marginal Tissue;Fibroblast Cell Line;Primary Explant Technique;Collagenase Technique;
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