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Occurrence of Glutathione Sulphydryl (GSH) and Antioxidant Activities in Probiotic Lactobacillus spp.
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Occurrence of Glutathione Sulphydryl (GSH) and Antioxidant Activities in Probiotic Lactobacillus spp.
Yoon, Yung H.; Byun, Jung R.;
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The antioxidative ability on the basis of reduced glutathione sulphydryl level, the inhibition activities of linoleic acid peroxidation of cell free extract of Lactobacillus spp. and the effects of types of media and growth phase of the cells on the cellular GSH level have been determined. Correlation between reduced glutathione sulphydryl level and antioxidative ability of Lactobacillus spp. was analyzed: Lactobacillus casei HY 2782 contained 25.15 mole/g of GSH, the cellular GSH level of L. casei HY 2782 reached maximum after 24 h of cultivation and tended to decrease on further cultivation up to 72 h. There was a significantly higher level of cellular GSH when grown in de Man Rogosa and Sharpe (MRS) broth than in tryptone phytone yeast extract (TPY) broth or bromcresol pruple dextrose (BCP) broth (p<0.05). The antioxidant activity of cell free extract of Lactobacillus spp. have been shown to be significantly different among strains in the inhibition of linoleic acid peroxidation by thiobarbituric acid (TBA) test (p<0.01). L. casei HY 2782 and L. acidophilus ATCC 4356 revealed a high degree of antioxidative effect in linoleic acid oxidation system. Spearmans' rank correlation coefficient between inhibitory activity on linoleic acid peroxidation and cellular GSH levels of Lactobacillus spp. was 0.65, which means a significant positive correlation.
GSH Level;Antioxidant Effect;
 Cited by
Anderson, M. 1985. Tissue glutathione: C.R.C. Handbook of methods for oxygen radical research. Boca Raton, Florida. CRC Press, Inc., 317-329.

Ahotupa, M., M. Saxelin and R. Kopela. 1996. Antioxidative porperiies of Lactobacillus GG. Nutr. Today (suppl.) 31:51S-52S.

Bonous, G., G. Batist and P. Gold. 1989. Immuno-enhancing property of dietary whey protein in mice: Role of glutathione. Clinical investigative Medicine 12:154-161.

Bounous, G. and P. Gold. 1991. The biological activity of undenatured whey proteins: The Role of glutathione. Clinical investigative Medicine 14:154-161.

Bounous. G., L. Letourneau and A. L. Patricia, Kongshavn. 1983. Influence of dietary protein type on the Immune system of mice. J. Nutr. 113:1415-1421.

Byun, J. R., Y. J. Baik and Y. H. Yoon. 2004. Effects of Feeding Lactobacillus spp. on the level of cell glutathione sulphydryl and immunoglobulin M in ICR mice. Asian-Aust. J. Anim. Sci. 17(3):415-419.

Demple, B., E. Hidalgo and H. Ding. 1999. Transcriptional regulation via redox-sensitive iron-sulphur centers in an oxidative stress response. Biochem. Soc. Symp. 64:119-128.

Fahey,.R. C., W. C. Brown, W. B. Brown and M. B. Worsham. 1978. Occurrence of glutathione in bacteria. J. Bacteriol. 133:1126-1129.

Halliwell, B. and J. M. C. Gutterridge. 1984. Oxigen toxicity,oxygen radicals, transition metals and disease. Biochem. J. 219, 1-4.

Johnston, C. S., C. G. Meyer and Srilankshmi. 1993. Vitamin C elevates red blood cell glutathione in healthy adults. Am. J. Clin. Nutr. 58:103-105.

Kullisaar, T., M. Zilmer, T. Vihalemm, H. Annuk, C. Kariane and A. Kilk. 2002. Two antioxidative Lactobacilli strains as promising probiotics. Int. J. Food Microbiol. 72:215-224.

Lin, M. Y. and C. L. Yen. 1999. Inhibition of lipid peroxidation by Lactobtacillus acidophilus and bifidobacterium longum. J. Agr. Food Chem. 47:3661-3664.

Perdigon, G., S. Alvarez, M. E. Nder de Macias, M. Roux and A. A. Pesce de Ruizholgade. 1990. The oral administration of lactic acid bacteria increase the mucosal intestinal immunity in response to enteropathogens. J. Food. Protect. 53:404-410.

Roederer, M., S. W. Ela, F. J. T. Stall and L. A. Herzenberg. 1992. A New Approach to anti HIV therapy. Pharmacology 46:121-129.

Rose, W. C. 1980. New aspects of glutathione biochemistry and Transport-Selective alteration of glutathione Metaboilsm. Nutrition Review/vol. 42. 12/December 397-420.

Salminen, S., M. Laine, A. Wright, Von, J. T. Voupiovarikila and T. Mattila-sandholm. 1996. Delevelopment of selection criteria for probiotic strain to assess their potential in functional food. a nordic and european approach. Bioscience Microfrala, 15:61-67.

Statistical Analysis System Institute, Inc. 1990. SAS Users Guide, Version S. SAS Institute Inc., Cary, NC.

Tammy, M. B. and C. G. Taylor. 1994. Enhanement of tissue glutathione for antioxidant and immune functions in malnutrition. Biochemical Pharcology. 47:2113-2123.

Vuyst, L. D. 1994. Bacteriocins in lactic acid bacteria. Blackil Academic & Professional. p. 353.

Yihong Cao, Zuliang Feng, Annie Hoos and V. Suzanne Klimberg. 1998. Glutamine enhances gut glutathione production. J. Parent. Eteral. Nutr. 22:224-227.

Yoon, Y. H. and B. R. Won. 2002. Antagonism against Helicobacter pylori and proteolysis of Lactobacillus helveticus CU 631 and Strain Identification. Asian-Aust. J. Anim. Sci. 15:1057-1065.