Advanced SearchSearch Tips
Production of Cloned Calves by the Transfer of Somatic Cells Derived from Frozen Tissues Using Simple Portable Incubator
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Production of Cloned Calves by the Transfer of Somatic Cells Derived from Frozen Tissues Using Simple Portable Incubator
Dong, Y.J.; Bai, X.J.; Varisanga, M.D.; Mtango, N.R.; Otoi, T.; Rajamahendran, R.; Suzuki, T.;
  PDF(new window)
The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -20, or in the presence of 5% DMSO used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstracted from the somatic cells, 62.3%, 76.6% to 65% showed cleavage 70.5%, 81.9% to 78.5% reached the stage of morula formation and 39.7%, 43.2% or 47.6% reached the blastocyst stage. There was no significant difference in development when the NT embryos were compared with those reconstracted from fresh somatic cell derieved skin tissues (72%, 75.3%, and 45.2%, for cleavage, and development to morula and blastocyst stage, respectively). NT embryos were then placed in a portable incubator and carried to China from Japan by air. After reaching to farm, two NT embryos were transferred to each of 5 recipients. We obtained 2 NT calves which birth weights is 30kg and 36kg female, and gestation periods is 281 and 284 days, respectively. There were no observation any abnormality from those calves. The results indicated that cell lines derieved from bovine fetal skin cryopreserved by a simple method could be used as donors in nuclear transfer using the portable incubator.
Cloning;Frozen Fetal Skin;Electrofusion;Portable Incubator;Blastocysts;Calves;
 Cited by
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection,;;

아세아태평양축산학회지, 2008. vol.21. 5, pp.648-656 crossref(new window)
Production of hand-made cloned buffalo (Bubalus bubalis) embryos from non-viable somatic cells, In Vitro Cellular & Developmental Biology - Animal, 2016, 52, 10, 983  crossref(new windwow)
Lyophilized somatic cells direct embryonic development after whole cell intracytoplasmic injection into pig oocytes, Cryobiology, 2010, 61, 2, 220  crossref(new windwow)
Arthur, G. H., D. E. Noakes and H. Pearson. 1989. Veterinary Reproduction and Obstetrics (Theriogenology), 6th ed. London: Bailliere Tindall. 49-160.

Bavister, B. D. Interactions between embryos and the culture milieu. 2000. Theriogenology 53: 619-628.

Campbell, K. H., J. McWhir, W. A. Ritchie and I. Wilmut. 1996. Sheep cloned by nuclear transfer from a cultured cell line. Nature, 380:64-66.

Campbell, K. H., P. Loi, P. Cappai and I. Wilmut. 1994. Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-phase of enucleated activated oocytes. Biol. Reprod. 50:1385-1393.

Cibelli, J. B., S. L. Stice, P. J. Golueke, J. J. Kane, J. Jerry, C. Blackwell, A. Ponce de Leon and J. M. Robl. 1998. Cloned transgenic calves produced from a fetal fibroblast cell line. Sci. 280:1256-1258.

Collas, P., Pinto-Correia, F. A. Ponce De Leon and J. M. Robl. 1992. Effect of donor cell cycle stage on chromatin and spindle morphology in nuclear transplant rabbit embryos. Biol. Reprod. 46:501-511.

Cox, S., J. Shaw and G. Jenkin. 1996. Transplantation of cryopreserved fetal ovarian tissue to adult recipients in mice. J. Reprod. Fertil. 107:315-322.

Gosden, R. G., M. I. Boulton, K. Grant and R. Webb. 1994. Follicular development from ovarian xenografts in SCID mice. J. Reprod. Fertil. 101:619-623.

Gunasea, K. T., J. R. T. Lakey, P. M. Villines, E. E. Critser and J. K. Critser. 1997. Allogenic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice. Biol. Reprod. 57:226-341.

Kato, Y., T. Tani, Y. Sotomaru, K. Kurokawa, J. Kato, H. Doguchi, H. Yasue and Y. Tsunoda. 1998. Eight calves cloned from somatic cells of a single adult. Sci. 282:2095-2098.

Kruip, T. and J. H. S. Den Daas. 1997. In vitro produced and cloned embryos: effects on pregnancy, parturition and offspring. Theriogenology 47:43-52.

Liu, J., J. Van der Elst, R. Van der Broecke and M. Dhont. 2001. Live offspring in vitro fertilization of oocytes from cryopreserved primodial mouse follicles after sequential in vitro transplantation and in vitro maturation. Biol. Reprod. 64:171-178.

Mazur, P. 1977. Slow-freezing injury in mammalian cells. Ciba Foundation Symposium . London. 52; p. 19-48.

McMillan, W. H., A. J. Peterson, D. R. H. Hall, M. Donnison, P. A. Pugh and M. G. Lambert. 1996. Is foetal loss random during pregnancy in cattle? In: The 13th Int Cong Anim Reprod, Sydney, Australia. 2: pp. 11-15 (abstract).

Menke, T. M. and A. McLaren. 1970. Mouse blastocysts grown in vivo and in vitro: carbon dioxide production and trophoblast outgrowth. J. Reprod. Ferti. 23:117-127.

Ogura, A., K. Inoue, N. Ogonuki, A. Noguchi, K. Takano, R. Nagano, O. Suzuki, J. Lee , E. Ishino and J. Matsuda. 2000. Production of male cloned mice from fresh cultured and cryopreserved immature Sertoli cells. Biol. Reprod. 62:1579-1584.

Peterson, A. J. and W. H. McMillan. 1998. Variation in the rate of emergence of the bovine allantois. In: Proc of the 29th Annual Conf of the Australian Soc for Repro Biol. Perth, Australia. p. 23 (abstract).

Suzuki, T., C. Sumantri, N. H. K. Khan, M. Murakami and S. Saha. 1999. Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos. Anim. Reprod. Sci. 54:149-157.

Tani, T., Y. Kato and Y. Tsunoda. 2000. Developmental potential of cumulus cells-derieved cultured cells frozen in a quiescent state after nuclear transfer. Theriogenology 53:1623-1629.

Tervit, H. R., D. G. Whittingham and L. E. A. Rowson. 1972. Successful culture in vitro of sheep and cattle ova. J. Reprod. Fertil. 30:493-497.

Van Wagtendonk-de Leeuw, A. M., B. J. G. Aerts and J. H. G. Den Daas. 1998. Abnormal offspring following in vitro production of bovine preimplantation embryos: a filed study. Theriogenology 49:883-894.

Varisanga, M. D., Y. J. Dong, N. R. Mtango, M. Fahrudin, K. K. Ni-wayan and T. Suzuki. 2000. Bovine blastocysts obtained from reconstructed cytoplast and karyoplasts using a simple portable ${CO}_2$ incubator. Cloning 4:167-173.

Varisanga, M. D., Y. J. Dong, N. R. Mtango and T. Suzuki. 2002. Comparison of the effects of using standard and portable ${CO}_2$ incubators on the in vitro developmental competence of bovine embryos reconstituted by somatic cell nuclear transfer. Theriogenology 58:77-86.

Wakayama, T., A. C. Perry, M. Zuccoti, K. R. Johson and R. Yanagimachi. 1998. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374.

Wells, D. N., P. M. Misica and H. R. Tervit. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60:996-1005.

Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind and K. H. S. Campbell. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813.