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Surgical Oocyte Retrieval and the Developmental Potential of the Oocytes Derived from Prepubertal Calves
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Surgical Oocyte Retrieval and the Developmental Potential of the Oocytes Derived from Prepubertal Calves
Yeh, S.P.; Ju, J.C.; Tseng, J.K.; Chou, P.C.; Chen, C.H.; Chih, T.; Fan, Y.K.;
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The objectives of this study were to investigate the ovarian responsiveness of juvenile calves to exogenous gonadotropin treatments and to establish the oocyte retrieval technique for prepubertal heifers. Three 78-day-old calves were treated with 4 doses (40, 30, 30 and 30 mg) of FSH (Folltropin V) at 12 h interval up to 229 day-old. Surgical oocyte retrieval was performed 24 h after the last injection of FSH. Calves with good ovarian responses to FSH treatment had an average ovarian size of cm compared to cm in the less-responsive animals. Large variations were observed in the number of total follicles (), aspirated follicles (), oocytes recovered () and usable oocytes recovered () during 78 to 229 day-old. Oocytes derived from prepubertal calves had significantly lower maturation rate than those from cows (34 vs. 100%, p<0.05). Mean diameters of calf oocytes () and ooplasm () were significantly lower than those of cows ( and , respectively). The diameter of the ooplasm also increased significantly after in vitro maturation (IVM) ( vs. ). However, further studies are required to optimize the IVP system for the oocytes derived from prepubertal heifers.
Oocyte;Gonadotropin;Calf;Surgical Retrieval;
 Cited by
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