Advanced SearchSearch Tips
A Quick Novel Method to Detect the Adulteration of Cow Milk in Goat Milk
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
A Quick Novel Method to Detect the Adulteration of Cow Milk in Goat Milk
Lee, Chi-Chei; Chang, His-Shan; Sheen, Hua-Shan;
  PDF(new window)
This study was to demonstrate a rapid novel method for detection of adulterated cow milk in goat milk using modified staining protocol after native polyacrylamide gel electrophoresis (PAGE). Samples of cow milk and goat milk containing 0, 0.5, 1.0 and 2.0% (v/v) of cow milk were analyzed. Low levels of cow milk mixed in goat milk were identified by the presence of higher mobility of -lactoglobulin A (-Lg A) in cow milk. By mini-gel electrophoresis, a distinguishable protein profile was visualized in 25 min using the modified Coomassie blue staining solution, in which methanol (50%) was replaced with ethanol (20%) and the concentrations of Coomassie blue and acetic acid were reduced from 2 to 0.13% and 10 to 5%, respectively. To visualize the milk proteins, gels in the staining solution were water-bathed in boiling water for 5 min and then cooled down immediately for 3-5 min. The sensitivity of this method is relatively high, allowing examination of 1% cow milk in goat milk. The procedure presented here is also very cost-effective due to less reagents needed. This simplified method would be useful and applicable to dairy industry for routine examination of goat milk.
 Cited by
MALDI-MS and multivariate analysis for the detection and quantification of different milk species, Analytical and Bioanalytical Chemistry, 2011, 399, 10, 3491  crossref(new windwow)
Detection of cow milk adulteration in yak milk by ELISA, Journal of Dairy Science, 2014, 97, 10, 6000  crossref(new windwow)
Amigo, L., M. Ramos, P. J. Martin-Alvarez and M. Barbosa. 1991. Effect of technological parameters on electrophoretic detection of cow’s milk in ewe’s milk cheeses. J. Dairy Sci. 74:1482-1490.

Anguitam, G., R. Martin, T. Garcia, P. Morales, A. I. Haza, I. Gonzalez, B. Sanz and P. E. Hernandez. 1997. A competitive enzyme-linked immunosorbent assay for detection of bovine milk in ovine and caprine milk and cheese using a monoclonal antibody against bovine $\beta$-casein. J. Food Prot. 60:64-66.

Bania, J., M. Ugorski, A. Polanowski and E. Adamczyk. 2001. Application of polymerase chain reaction for detection of goats’ milk adulteration by milk of cow. J. Dairy Res. 68:333-336.

Cattaneo, T. M. P., F. Nigro and G. F. Greppi. 1996. Analysis of cow, goat and ewe milk mixtures by capillary zone electrophoresis (CZE): preliminary approach. Milchwiss. 51:616-619.

Douglas, L. H. and M. G. Zeece. 1988. Two-dimensional electrophoresis of bovine milk proteins. J. Dairy Sci. 71:2044-2050.

El Ghannam, M. S. 1994. Detection of mixtures of buffaloes’, cows’ and goats’ milk using polyacrylamide gel electrophoresis. Alexandria J. Agric. Res. 39:171-182.

Iverson, J. L. and A. J. Sheppard. 1989. Detection of adulteration in cow, goat, and sheep cheeses utilizing gas-liquid chromatographic fatty acid data. J. Dairy Sci. 72:1707-1712.

Jenness, R. 1980. Composition and characteristics of goat milk:review 1968-1978. J. Dairy Sci. 63:1605-1630.

Liang, I. and S. N. Huang. 1999. Detection of cow’s milk in goat’s milk by urea polyacrylamide gel electrophoresis. J. Taiwan Livest. Res. 32: 153-159.

Romero, C., O. P. Andujar, A. Olmedo and S. Jimenez. 1996. Detection of cow’s milk in ewe’s or goat’s milk by HPLC. Chromatogr. 42:181-184.

Sibel, F., K. Akpinar and Z. Soylemez. 1996. Identification of milk from goat, ewe and cow in their mixtures by polyacrylamide gel electrophoresis. J. Food Qual. 19:391-393.

Szijarto, L. and F. R. van de Voort. 1983. Determination of added water and bovine milk to caprine milk. J. Dairy Sci. 66:620-623.