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Optimization of the Viability of Probiotics in a Fermented Milk Drink by the Response Surface Method
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 Title & Authors
Optimization of the Viability of Probiotics in a Fermented Milk Drink by the Response Surface Method
Chen, Ming-Ju; Chen, Kun-Nan; Lin, Chin-Wen;
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Growth promoters were added to skim milk to retain the viability of Lactobacillus acidophilus and Bifidobacterium longum to help the product meet the "therapeutic minimum" at the time of consumption. The experiments were divided into two parts. The first part of the study used chicory inulin, isomalto-oligosaccharides and sucrose to investigate the effects of sugars on the activity of L. acidophilus and B. longum. The results indicated that the addition of isomalto-oligosaccharides stimulated growth of L. acidophilus and B. longum, resulting in a higher level of the probiotics after one month storage and yielded better -galactosidase activity during fermentation. The second part studied the effects of three growth promoters on the viability of the probiotic cultures and the response surface method was employed to find the optimal ratio for addition of the growth promoters. The optimal ratio for added calcium gluconate, sodium gluconate and N-acetylglucosamine in fermented milk drinks were established. The response surface method proved to be a very effective way of optimizing the activity of probiotic cultures when developing a new fermented milk drink.
Probiotics;Response Surface Method;Fermented Milk;
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Chen, M. J., K. N. Chen and C. W. Lin. 2003. Optimization of the growth rate of probiotics in fermented milk using genetic algorithms and sequential quadratic programming techniques. Asian-Aust. J. Anim. Sci. 16:894-902.

Chen, M. J. and C. W. Lin. 2002. Factors affecting the waterholding capacity of fibrinogen/plasma protein gels optimized by response surface methodology. J. Food Sci. 67(7):2579-2582.

Fooks, L. J., R. Fuller and G. R. Gibson. 1999. Prebiotics, probiotics and human gut microbiology. Int. Dairy J. 9:53-61.

Kanbe, M. 1992. Functions of fermented milk: Challenges for the health sciences (Ed Y. Nakazawa and A. Hosono), Elsevier Appl. Publ., London.

Klaver, F. A. M., F. Kingma and A. H. Weerkamp. 1993. Growth and survival of Bifidobacteria in milk. Neth. Milk. Dairy J. 47:151-164.

Kleinbaum, D. G., L. L. Kupper, K. E. Muller and A. Nizam. 1998. Applied regression analysis and multivariable methods. 3rd ed. Duxbury Press. New York, USA.

Kneifel, E., D. Jaros and F. Erhard. 1993. Microflora and acidification properties of yogurt and yogurt-related products fermented with commercially available starter cultures. Int. J. Food Microbiol. 18:179-189.

Kohmoto, T., F. Kukui, H. Takaku, Y. Machida, M. Arai and T. Mitsuoka. 1988. Effect of isomalto-oligosaccharides on human faecal flora. Bifidobacteria Microflora 7:61-69.

Hamann, E. T. and E. H. Marth. 1983. Survival of Streptococcus thermophilus and Lactobacillus bulgaricus in commercial and experimental yogurts. J. Food Prot. 47(10):781-786.

Lankaputhra, W. E. V. and N. P. Shah. 1994. Investigation of factors affecting viability of Lactobacillus acidophilus and Bifidobacteria in yogurt. 24th Inter. Dairy Congress, Melbourne, Australia, Sept. 18-22.

Lapierre, L., P. Undeland and L. J. Cox. 1992. Lithium Chloride- Sodium Propionate agar for the enumeration of bifidobacteria in fermented dairy products. J. Dairy Sci. 75:1192-1196.

Liu, Y. C., M. J. Chen and L. W. Lin. 2002. Studies on Lao-Chao culture filtrate for a flavoring agent in a yogurt-like product. Asian-Aust. J. Anim. Sci. 15(3):172-179.

Lourens-Hattingh, A. and C. Viljoen. 2001. Yogurt as probiotic carrier food. Int. Dairy J. 11:1-17.

Mitsuoka, T., H. Hidaka and T. Eida. 1987. Effect of oligosaccharides on intestinal microflora. Die Nahrung 31:427-436.

Marx, S. P., S. Winkler and W. Hartmeier. 2000. Metabolization of $\beta$-(2,6)-linked fructose-oligosaccharides by different bifidobacteria. FEMS Microbiol. Lett. 182:163-169.

Montgomery, D. C. 1991. Experilments with a single factor:the analysis of variance. Design and Analysis of Experiments, 3rd ed, pp. 75-77. John Wiley & Son. Publ. New York, USA.

Playne, M. 1994. Probiotic foods. Food Australia 46(8):362.

Myers, R. H. and D. C. Montgomery. 1995. Response surface methodology: Process and product optimization using designed experiments. John Wiley & Son. Publ., New York, USA.

SAS Institute Inc. 1990. SAS/STAT User's guide. SAS Institute Inc., North Carolina, USA.

Scheinbach, S. 1998. Probiotics: functionality and commercial status. Biotechnology Advances 16 (3):581-608.

Vinderola, C. G. G. A. Costa, S. Rdgenhardt and J. A. Rdinheimer. 2002. Influence of compounds associated with fermented dairy products on the growth of lactic acid starter and probiotic bacteria. Int. Dairy J. 12:579-589. crossref(new window)

Young, C. K. and F. E. Nelson. 1978. Survival of Lactobacillus acidophilus in 'Sweet Acidophilus Milk' during refrigerated storage. J. Food Prot. 41(4):248-250.

Yu, P.-L., J. B. Smart and B. M. Ennis. 1987. Differential induction of $\beta$-galactosidase and phospho-$\beta$-galactosidase activity in the fermentation of whey permeate by Clostridium acetobutylicum. Applied Microbiology and Biotechnology 26:254-257.

Ziemer, C. J. and G. R. Gibson. 1998. An overview of probiotics, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. Int. Dairy J. 8:473-479.