Advanced SearchSearch Tips
Quality and Fertility of Post Thaw Sephadex Filtered Bull Semen
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Quality and Fertility of Post Thaw Sephadex Filtered Bull Semen
Kumar, A.; Singh, J.; Nanda, A.S.; Pangaonkar, G.R.;
  PDF(new window)
The present investigation was carried out to assess the effect of Sephadex (G-15) filtration on the post thaw bull semen quality and conception rate. Post thaw unfiltered (control) and Sephadex filtered semen from four healthy bulls (three cross bred and one pure bred Holstein Friesian) were subjected to microscopic examination viz. sperm concentration, individual motility, live sperm count and sperm morphology. Sixty-two healthy, normal cycling crossbred cows were inseminated with post thaw unfiltered (n=32) and filtered semen (n=30). Sephadex filtration of post thaw semen significantly (p<0.05) decreased total sperm concentration and sperm with abnormal head, mid piece and tail. The overall average total sperm concentration, head and tail defects in filtered semen decreased significantly (53.4, 1.2 and 6.4 million) than in the unfiltered semen (80.4, 2.4 and 15.7 million, respectively). However, after filtration significant (p<0.05) increase in overall average motile and live sperm concentration were observed (38.8 and 38.0) as compared to unfiltered semen (29.2 and 32.0 million, respectively). The overall conception rate recorded was 21.9% with post thaw unfiltered semen and 56.7% with filtered semen. It was concluded that Sephadex filtration of post thaw semen improved its quality and conception rate.
Bull Semen;Sephadex Filtration;Fertility;
 Cited by
Agrawal, A., B. S. Ann Manglona, R. Kevin and Loughlin. 1991. Filtration of spermatozoa through L4 membrane: A New Method. Fertil Steril. 56:1162-1165.

Aitken, R. J. and J. S. Clarkson. 1989. Significance of reactive oxygen species and antioxidants in defining the efficiency of sperm preparation techniques. J. Androl. 9:367.

Alvariz, J. G., J. C. Touchstone, L. Blasco and B. T. Storey. 1987. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. J. Androl. 8:334-348.

Baker, R. D. and A. A. Degen. 1972. Transport of live and dead boar spermatozoa in their reproductive tract of gilts. J. Reprod. Fertil. 28:369-377.

Blom, E. 1977. The evaluation of bull semen with special reference to its use in artificial insemination. Anim. Breed Abstr. 19:648 (Abstr.).

Chauhan, S. S., Greesh, Mohan, K. Satish and K. L. Sahni. 1993. Effect of Sephadex filtration in improving the quality of buffalo semen during storage and freezing. Indian J. Anim. Sci. 63:1036-1041.

De, B. N., A. Chatterjee, J. Bidyanta, M. Chakarborty, S. K. Deb, P. Mondal and G. P. Sen. 1982. Note on problems of breeding cows with special reference to common coital infections. Indian J. Anim. Sci. 52:700-702.

Estiennen, M. J., J. W. Knight and W. E. Beal. 1988. Isolation of a population of intact, highly motile porcine spermatozoa using a discontinuous bovine serum albumin gradient. Theriogenology 29:771-778.

Fayami, E. O., B. G. Carbo and E. P. Graham. 1979. Assay of frozen boar semen with Sephadex filrtation. Theriogenology 12:13-17.

Fonseca, V. O., V. R. Vale Filho, L. A. Chow, J. A. G. Bergmann and T. I. Assumpcao. 1995. Effect of sperm concentration on conception rate in zebu cows (Bos taurus indicus). Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 47:687-697.

Graham, E. F. and J. K. Graham. 1990. The effect of whole ejaculate filtration on the morphology and the fertility of bovine semen. J. Dairy Sci. 73:91-97.

Graham, E. F., M. K. L. Schnell, L. A. Vanquez and B. K. Evenson. 1976. An assay of semen quality by use of Sephadex filtration. Proc. 8th Int. Congr. on Animal Reproduction and Artificial Insemination. pp. 95-96. Agricultural University, Warszawa, Kvakow, Poland.

Howalader, M. M. R., M. F. Mian, A. H. M. Kamal, M. A. M. Prodhan and M. F. Rahman. 1997. Conception rate of Pabna cows and heifers bred under artificial insemination and natural service. Asian-Aust. J. Anim. Sci. 10:329-332.

Kanakraj, P., B. M. Easwaran and A. R. Krishnan. 1996. Sephadex column filtration in improving the quality of bull semen. Indian J. Anim. Sci. 11:125-127.

Kodoma, H., R. Yamaguchi, J. Fukuda, H. Kasai and T. Tanaka. 1997. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril. 68:519-524.

Kommisrud, E., T. Steine and T. Graffer. 1996. Comparison of fertility rates following insemination with different numbers of spermatozoa per insemination dose of frozen bovine semen. Reprod. Domestic Anim. 31:359-362.

Krishnamurti, G. V., R. D. Nanjiah and B. S. Keshavmurthy. 1974. Bacterial flora of cervical mucus in repeat breeding bovine. Indian Vet. J. 51:254-268.

Kumar, N., J. Singh and G. R. Pangaonkar. 1992. A new technique to improve the post thaw semen quality of buffalo bulls. Indian Vet. Med. J. Vol. 16:47-50.

Linda, C. A., J. O. Almiquist and R. P. Aman. 1980. Factors influencing Sephadex filtration of bovine and ovine spermatozoa. J. Dairy Sci. 63:277-282.

Lodhi, L. A. and B. G. Crabo. 1984. Filtration of bull spermatozoa through Sephadex, polyacrylamide, silica gel and glass wool in presence and absence of two sugars. Proc 10th Int Congr on Animal Reproduction and Artificial Insemination. p. 59. Orbana-Champaign, Illinois, USA.

Luderer, A. A., W. W. Dean, A. R. Zine, D. M. Hess, R. H. Foote and R. J. Wall. 1982. Separation of bovine spermatozoa by density on water insoluble newtonian gel and their use for insemination. Biol. Reprod. 26:813.

Maki-Laurila, M. and E. F. Graham. 1968. Separation of dead and live spermatozoa in bovine semen. J. Dairy Sci. 51:965.

Markvichitr, K., P. Innuraksa, S. Tumwasorn and S. Kaewsuwan. 1997. Reproductive and growth performance test of cross bred beef bulls (1/2-Charolais 1/4 Brahman 1/4-native) for beef production efficiency improvement in Thailand. Kasetsart Journal, Natural Sciences 31:297-304.

Maurya, S. N. 2000. Animal Reproduction Scenario in India: Role of Gynaecologists. Indian J. Anim. Reprod. 21:85-91

McGrath, J., N. Hillman and M. Nodijcka. 1977. Separation of dead and live mouse spermatozoa. Dev Biol. 61:114.

Nair, B. R. Krishnan. 1975. A study on the conception rate in cattle due to insemination with deep frozen semen. Indian Vet. J. 52: 165-169.

Paulson, J. D. and K. L. Polakoski. 1977. A glass wool column procedure for removing extraneous material from human ejaculate. Fertil Steril. 28:178-181.

Rankin, T. A., W. R. Smith, R. D. Shanks and J. R. Lodge. 1992. Timing of insemination in dairy heifers. J. Dairy Sci. 75:2840-2845.

Roberts, A. M. 1972. Gravitational separation of X and Y spermatozoa. Nature. 238:223-225. crossref(new window)

Romanoff, A. L. and A. J. Romanoff. 1949. The Avian Egg. John Wiley & Sons. New York City.

Saacke, R. G. 1970. Morphology of sperm and its relationship to fertility (Cattle mainly). Proc. 3rd Natl. Assoc. Anim. Breeders Tech. Conf. Artif. Insem. Reprod. p. 17.

Sahu, S. B. and R. K. Pandit. 1997. Effect of cryopreservation of sperms of Murrah bulls. Indian J. Anim. Reprod. 18:137-138.

Shannon, P. and B. Curson. 1972. Toxic effect and action of dead sperms on diluted bovine semen. J. Dairy Sci. 55:614-620.

Sharma, N. C. 1987. Artificial Insemination of Cattle and Buffaloes. pp. 30-32. Anupam Printers, Baraily, India.

Singh, S. P., R. K. Pandit and H. B. S. Bhadoria. 1997. Effect of freezing on seminal characteristics of Jersey, Sahiwal their half-bred bulls. Indian J. Anim. Reprod. 18:73-74.

Sofikitis, N., I. Miyagawa, P. M. Zavos, S. Jnaga, A. Iino, T. Toda, T. Harada, Y. Mio and N. Terakawa. 1992. Acrosin profiles of human spermatozoa recovered from the new sperm Prep filtration column. Tohoku J. Exp. Med. 166:451-457.

Tilney, L. G., S. Hatano, H. Ishikawa and M. S. Mooseker. 1973. The polymerization of actin: its role in the generation of the acrosomal process of certain echinoderm sperm. J. Cell Biol. 59:109-126.

Vyas, S., M. Greesh, A. J. Dhami and K. L. Sahni. 1991. Effect of Sephadex and glass wool column filtration on the quality and storage ($5^{\circ}C$) of cross-bred bull semen. Indian J. Anim. Sci. 6:702-704.

Wall, R. J., D. A. Jerrard, J. J. Parrish and R. H. Foote. 1984. Optimization of procedures for separation of motile and nonmotile bull and rabbit spermatozoa with bovine semen albumin gradients. Gamete Res. 10:165.