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Influence of an Anaerobic Fungal Culture (Orpinomyces sp.) Administration on Growth Rate, Ruminal Fermentation and Nutrient Digestion in Calves
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 Title & Authors
Influence of an Anaerobic Fungal Culture (Orpinomyces sp.) Administration on Growth Rate, Ruminal Fermentation and Nutrient Digestion in Calves
Dey, Avijit; Sehgal, Jatinder Paul; Puniya, Anil Kumar; Singh, Kishan;
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The study was to see the effect of administration of ruminal fungi on feed intake, growth rate, rumen fermentation and nutrient digestion of calves (TharparkarHolstein-Friesian, average age: 10 months, average body weight: 130 kg). The 6 calves in first group were fed a mixture consisted of 50% wheat straw and 50% concentrate (Maize 62%, Groundnut cake 35%, Mineral mix. 2% and Common salt 1%) along with 1 kg green oats while second group calves were fed the above-mentioned diet in addition to a dose of 160 ml ( CFU/ml) fungal culture . The average dry matter intake per day was slightly lowered in fungal fed calves yet feed conversion ratio was higher. The average daily weight gain was significantly higher (15.37%) in fungal administered group as compared to control. The nutrient digestibility was increased for crude fibre, NDF and ADF with fungal administration. Digestible energy value of straw-based diet in terms of percent TDN also increased. The pH and -N were lower whereas TVFA, total-N, TCA-N and number of zoospores were higher in rumen liquor in fungal administered group.
Anaerobic Ruminal Fungi;Orpinomyces sp.;Growth Rate;Nutrient Digestion;
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Akin, D. E., G. L. R. Gordon and J. P. Hogan. 1983. Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulphur. Appl. Environ. Microbiol. 46:738-748.

Akin, D. E., L. L. Rigsby, C. E. Lyon and W. R. Windham. 1990. Relationship of tissue digestion to textural strength in bermnda grass and alfalfa stems. Crop Sci. 30:990-993.

AOAC. 1992. Official Methods of Analysis. 16th edn. Association of official analytical chemists, Washington, DC.

Barnett, A. J. G. and R. L. Reid. 1956. Studies on the production of volatile fatty acids from the grass by rumen liquor in an artificial rumen. J. Agric. Sci. 48:315-321.

Bhatia, S. K., S. Prahalad, K. Pradhan and D. P. Sharda. 1982. Note on the effect of guar meals on intra ruminal metabolism of protein and microbial transaminase activity in crossbred cattle. Indian J. Anim. Sci. 52:800-802.

Borneman, W. S., L. G. Ljungdahl, R. D. Hartley and D. E. Akin. 1992. Purification and partial characterization of two feruloyl esterases from the anaerobic fungi in relation to plant cell wall degradation. Appl. Microbiol. Biotech. 33:345-351.

Broody, S. 1945. Bioenergetics and growth. Reinhold Publ. Corp., New York, USA.

Bryant, M. P. and L. A. Burkey. 1953. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci. 36:205-217.

Chang, J. S. and R. E. Calza. 2002 Effect of Cicer Milkvetch (Astragalus cicer) extracts on physiology of rumen fungus: Neocallimastix frontalis EB188. Asian-Aust. J. Anim. Sci. 15:1156-1164.

Conway, E. J. 1962. Micro-diffusion analysis and volumetric error. 5th edn. Crossby Lockwood and Sons Ltd., London.

Goering, H. K. and P. J. VanSoest. 1970. Forage fibre analysis. Agricultural Handbook No. 379, USDA, Washington, DC.

Gordon, G. L. R. and M. W. Phillips. 1993. Removal of anaerobic fungi from the rumen of sheep by chemical treatment and the effect on feed consumption and in vivo fibre digestion. Lett. Appl. Microbiol. 17:220-223.

Gordon, G. L. R. and M. W. Phillips. 1998. The role of anaerobic gut fungi in ruminants. Nutr. Res. Rev. 11:1-36.

Gordon, G. L. R., M. W. Phillips, A. J. Rintout and S. W. White. 2000. Increased intake of fibrous feed by sheep orally dosed with a culture of and elite non-indigenous anaerobic gut fungus. Asian-Aust. J. Anim. Sci. 13 (Suppl) p. 13.

Ha, J. K., S. S. Lee, C. H. Kim, Y. J. Choi and H. K. Min. 1994. Effect of fungal inoculation on ruminal fermentation characteristics, Enzyme activities and nutrient-digestion in sheep. Proc. Soc. Nutr. Physiol. 3:197.

Ho, Y. W., N. Abdullah and S. Jalaludin. 1996. Microbial colonization and degradation of some fibrous crop residues in the rumen of goats. Asian-Aust. J. Anim. Sci. 9:519-524.

Hume, I. D., R. J. Moir and M. Somers. 1970. Synthesis of microbial protein in the rumen. 1. Influence of the level of nitrogen intake. Aust. J. Agri. Res. 21:283-296.

Hungate, R. E. 1969. A roll tube method for cultivation of strict anaerobes. Methods Microbiol. 3B:117-132.

Joblin, K. N. 1981. Isolation, enumeration and maintenance of rumen anaerobic fungi in roll tubes. Appl. Environ. Microbiol. 42:1119-1122.

Johnson, V. W. and J. D. Sutton. 1968. The continuous recording of the pH in the bovine rumen. Br. J. Nutr. 22:303-306.

Kostiukovskii, V. A., O. N. Okunev and B. V. Tarakanov. 1990. Anaerobic cellulolytic fungi from cattle rumen. Mikrobiologiia 59:1067-1074.

Lane, G. T., C. H. Nellor, V. F. Colenbrander, K. R. Cummings and R. B. Harrington. 1968. Apparatus for obtaining ruminoreticular samples and the effect of sampling locations on pH and volatile fatty acids. J. Dairy Sci. 51:114-116.

Lee, S. S., J. K. Ha and K. J. Cheng. 2000. Influence of an anaerobic fungal culture administration on in vivo ruminal fermentation and nutrient digestion. Anim. Feed Sci. Technol. 88:201-217.

Lee, S. S., J. K. Ha and K. J. Cheng. 2001. Effects of LCFA on the gas production cellulose digestion and cellulase activities by the rumen anaerobic fungus Neocallimastix frontalis RE 1. Asian-Aust. J. Anim. Sci. 14:1110-1117. crossref(new window)

Manikumar, B., A. K. Puniya and K. Singh. 2002. Influence of ruminal Fungi on degradation of wheat straw in vitro. Indian J. Microbiol. 42:133-136.

Manikumar, B., A. K. Puniya and K. Singh. 2003. Effect of ruminal fungi on in vitro degradation of rice straw. Indian J. Anim. Sci. 73:312-314.

Mehrez, A. Z., E. R. Orskov and I. McDonald. 1977. Rates of rumen fermentation in relation to ammonia concentration. Br. J. Nutr. 38:437-443.

Miller, E. L. 1973. Evaluation of food as source of N and amino acids. Proc. Nutr. Soc. 32:79-84. crossref(new window)

NRC. 1989. Nutrient requirement of calves. National Academy of Science, Washington, DC.

Oka, A., T. Dohogo, S. Ohtagaki and M. Juen. 1999. Effects of roughage levels on growth, beef quality, ruminal contents and serum constituents in Japanese Black steers during the growing period. Anim. Sci. J. 70:451-459.

Orpin, C. G. 1975. Studies on the rumen flagellate Neocallimastix frontalis. J. Gen. Microbiol. 91:249-262. crossref(new window)

Paul, S. S., D. N. Kamra, V. R. B. Sastry, N. P. Sahu and A. Kumar. 2003. Effect of phenolic monomers on biomass and hydrolytic enzyme activities of an anaerobic fungus isolated from wild nil gai (Baselophus tragocamelus). Lett. Appl. Microbiol. 36:377-381 crossref(new window)

Phillips, M. W. and G. L. R. Gordon. 1995. Carbohydrate fermentation by three different polycentric rumen fungi from cattle and water buffalo in tropical Australia. Anaerobe. 1:41-47.

Punia, B. S. and D. D. Sharma. 1988. Effect of dietary energy source in complete feed on digestion and live weight gain in buffalo and crossbred cattle calves. Indian J. Anim. Sci. 58:715-720.

Samanta, A. K., T. K. Walli and K. K. Singh. 2001. Role of different groups of microbes on fibre utilization. Indian J. Anim. Sci. 71:497-498.

Sehgal, J. P., D. D. Sharma, M. K. Ghosh and K. K. Singhal. 1999. Fattening of male buffalo calves for veal production. Buffalo J. 2:235-239.

Singhal, A. 2000. Characterization of rumen fungi and their genetic analysis. M.Sc. Thesis, Dairy Microbiology Division, National Dairy Research Institute, Karnal, India.

Snedecor, G. W. and W. G. Cochran. 1980. Statistical Methods. 7th edn. The Iowa State University Press, Iowa, USA.

Theodorou, M. K., M. K. Gill, C. King-Spooner and D. E. Beever. 1990. Enumeration of anaerobic chytridiomycetes as thallus forming units: A novel method for the quantification of fibrolytic fungal populations from the digestive tract ecosystem. Appl. Environ. Microbiol. 56:1073-1078.