Advanced SearchSearch Tips
Lectin Activity and Chemical Characteristics of Escherichia coli, Lactobacillus spp. and Bifidobacterium spp. from Gastrointestinal Mucosa of Growing Pigs
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Lectin Activity and Chemical Characteristics of Escherichia coli, Lactobacillus spp. and Bifidobacterium spp. from Gastrointestinal Mucosa of Growing Pigs
Gao, W.; Meng, Q.X.;
  PDF(new window)
Lectin activities and chemical characteristics of Escherichia coli, Lactobacillus spp. and Bifidobacterium spp. originating from the porcine cecal mucosal layer were studied based on hemagglutination assay (HA) and hemagglutination inhibition assay (HIA). Although all the bacterial strains were able to agglutinate erythrocytes of porcine or rabbit origin, much higher HA titers were consistently observed for Lactobacillus spp. than for E. coli or for Bifidobacterium spp. A remarkable reduction in HA titers occurred by the treatment of E. coli and Lactobacillus spp. with protease or trypsin and of Bifidobacterium spp. with protease, trypsin or periodate. There were no significant effects on the HA titers of the three groups of bacteria after the treatment with lipase. Hemagglutination of E. coli was strongly inhibited by D (+)-mannose and D (+)-galactose; Lactobacillus spp. by -L-rhamnose and methyl--galactopyranoside; Bifidobacterium spp. by D (+)-alactose, -L-rhamnose, -L-fucose, L (+)-arabinose, D (+)-mannose, D (-)-fructose at a relatively low concentration (1.43 to 3.75 mg/ml). These results, combined with the enhanced HA activities of the three bacterial strains by modification of rabbit erythrocytes with neuraminidase and abolished HA activity of E. coli after treatment with -galactosidase, indicate that it might be the glycoproteinous substances surrounding the surface of the bacterial cells that are responsible for the adhesions of these microorganisms by recognizing the specific receptors on the red blood cell.
Indigenous Gastrointestinal Bacteria;Escherichia coli;Lactobacillus spp.;Bifidobacterium spp.;Lectins;Growing Pigs;
 Cited by
Alm, L., C. E. Leijonmarok, A. K. Person and T. Midtvedt. 1989. Survival of lactobacilli during digestion: An in vitro and in vivo study. In: Regulatory and Protective Role of the Normal Microflora (Ed. R. Grubb, T. Midtvedt and E. Norin). Stockton Press, New York. pp. 293-297.

Bernet, M. B., D. Brassart, J. Neeser and A. L. Servin. 1993. Adhesion of human bifidobacteria strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions. Appl. Environ. Microbiol. 59:4121-4128.

Chapman, J. D. 1988. Probiotics, acidifiers and yeast culture: A place for natural additives in pig and poultry production. In: Biotechnology in the Feed Industry (Ed. T. P. Lyons and K. A. Jacques). Proc Alltech's 4th Annu Symp Alltech Technical Publications, Kentucky. pp. 219-233.

Goldstein, I. J., R. C. Hughes, M. Monsigny, T. Osawa and N. Sharon. 1980. What should be called a lectin? Nature (Lond.).285:66.

Henriksson, A., R. Sezwzyk and P. L. Conway. 1991. Characteristics of the adhesive determinants of Lactobacillus fermentum 104. Appl. Environ. Microbiol. 57:499-502.

Hentges, D. J. 1992. Gut flora and diseases resistance. In: Probiotics: The Scientific Basis (Ed. R. Fuller). Chapman & Hall, UK. pp. 87-110.

Hungate, R. E. 1969. A roll-tube method for cultivation of strict anaerobes. Methods. Microbiol. 3B:117-132.

Meng, Q. and R. L. Preston. 1997. Evidence for existence of lectins on the ruminal bacteria from steers fed roughage and concentrate diets. J. Gen. Appl. Microbiol. 43:81-87.

Meng, Q. and R. L. Preston. 1998. Factors influencing bacterial attachment to the ruminal epithelium in vitro. J. Gen. Appl. Microbiol. 44:35-41.

Meng, Q., M. S. Kerley, T. J. Russel and G. L. Allee. 1998. Lectins-like activity of Escherichia coli K88, Salmonella choleraesuis and Bifidobacterium pseudolongum originated from porcine gastrointestines. J. Anim. Sci. 76:551-557.

Mukai, T., K. Arihara and H. Itoh. 1992. Lectin-like activity of Lactobacillus acidophilus strain JCM 1026. FEMS. Microbiol. Lett. 98:71-74.

SAS. 1996. SAS User’s Guide. Statistical System Institute, Inc. Cary, NC.

Savage, D. C. 1977. Microbial ecology of the gastrointestinal tract. Annu. Rev. Microbiol. 31:107-133. crossref(new window)

Sharon, N. 1987. Bacterial lectins, cell-cell recognition and infectious disease. FEBS Lett. 217:145-157.

Sharon, N. and H. Lis. 1972. Lectins: Cell agglutinating and sugar specific proteins. Sci. 177:949-959.

Simon, G. L. and S. L. Gorbach. 1986. The human intestinal microflora. Dig. Dis. Sci. 31:147S.

Stavric, S. and E. T. Kornegay. 1995. Microbial probiotics for pigs and poultry. In: Biotechnology in Animal Feeds and Animal Feeding (Ed. R. J. Wallace and A. Chesson). VCH Publishers, New York. pp. 205-231.

Stewart, C. S. 1996. Microorganisms in hindgut fermentors. In: Gastrointestinal Microbiology, Volume 2 Gastrointestinal Microbes and Host Interactions. (Ed. R. I. Mackie, B. A. White and R. E. Isaacson). Chapman & Hall, New York. pp. 142-185.

Tocuda, G. and R. E. Warringtong. 1970. Detection of foot and mouth disease virus antibodies: I. 'Passive' hemagglutination test. Appl. Microbiol. 20:35-39.

Wadstrom, T., K. Andersson, M. Sydow, L. Axelsson, S. Lindgren and B. Gullmar. 1987. Surface properties of lactobacilli isolated from the small intestine of pigs. J. Appl. Bacteriol. 62:513-520.

Yoon, Y. H. and B. R. Won. 2002. Antagonism against Helicobacter pylori and proteolysis of Lactobacillus helveticus CU631 and strain identification. Asian-Aust. J. Anim. Sci. 15:1057-1065.