JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Certain Immunomodulators on Uterine Infections and Fertility in Post Partum Buffaloes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Certain Immunomodulators on Uterine Infections and Fertility in Post Partum Buffaloes
Kumar, Puneet; Srivastava, S.K.; Rawat, Mayank; Yadav, M.C.; Kumar, H.;
  PDF(new window)
 Abstract
The present study was aimed to study the effect of aqueous extract of Tinospora cordifolia and autologous plasma on uterine infections. Buffaloes in estrus, 40 days and above postpartum were checked for uterine infections. Animals having uterine infections were randomly divided into three groups of six animals each. Buffaloes in group I, II and III (control) were given intrauterine infusion of aqueous extract of Tinospora cordifolia (3,000 mg total dose), autologous plasma (150 ml) and phosphate buffer saline (150 ml) respectively, in three divided doses, once daily for three consecutive days, starting from the day of estrus. A fourth group (IV) comprising of six buffaloes having no uterine infection was also included in the present study. Buffaloes were inseminated artificially on next estrus following treatment and confirmed for pregnancy 60 days later. Bacterial population in CVM of buffaloes in group I, II and III was significantly (p<0.05) higher than group IV. After treatment there was a significant (p<0.01) reduction in bacterial population in group I (83.4967.755%) and group II 80.2335.799%) than group in III 7.55733.551%) at next estrus. There was non-significant improvement in first service conception rate (CR, 33.33%) and overall conception rate (OCR, 27.27%) in group I, in comparison to group III (first service CR-16.67%; OCR-20.0%). No significant improvement was seen in OCR (22.22%) in-group II also in comparison to group III. The improvement in group I was however, nonsignificantly lower than normal animals of group IV (First service CR-16.67%; OCR-36.33%).
 Keywords
Buffaloes;Uterine Infection Tinospora cordifolia;Autologous Plasma;Bacterial Load;Conception Rate;
 Language
English
 Cited by
 References
1.
Adhau et al., 1999. Bacteriological isolates in repeat breeder buffaloes and treatment. Indian J. Anim. Sci. 69(7):504-506.

2.
Arora, A. K., Singh Jagir, G. R. Pangaonkar and A. S. Nanda. 2000. Bacteriological studies on the genital infection in repeat breeder bovines. Indian J. Anim. Reprod. 21(2):146-147.

3.
Asbury, A. C. 1984. Uterine defence mechanisms in the mare: the use of intrauterine plasma in managment of endometritis. Theriogenology 21:387-393.

4.
Asbury, A. C., R. I. W. Hallwell and G. W. Foster. 1980. Immunoglobulins in uterine secretions of mares with differing resistance to endometritis. Theriogenology 14:299-308.

5.
Boitor, I., M. Muntean, N. Mutes, D. Moise, R. Morar, M. Boitor, C. Sahleanu and L. Gorgan. 1980. Laboratory and therapeutics studies of puerperal endometritis and repeat breeding in cows. Buletinul Institula Agronomic Clug-Napoea Zootechnic Se Medicina Veterinaria, 34:111-114.

6.
Cowan, S. T. 1974. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge and London, Cambridge University Press.

7.
Dhaliwal, G. S., R. D. Murray and R. Woldehiwet. 2001. Some aspects of immunology of bovine uterus related to treatments for endometritis. Anim. Repro. Sci. 67:135-152.

8.
Direkbusarakom, S., Y. Ezura, M. Yoshimizu and A. Herunsalec. 1998. Efficacy of Thai traditional herb extracts against fish and shrimp pathogenic bacteria. Proceedings of the international symposium on diseases in Marine aquaculture, Hiroshima, 3-6 Oct., 1997 Gyobyo-Kenkyu. Fish Pathology 33(4):437-389.

9.
Feingold Davis, S., N. Goldman John and M. Kuritz Harold. 1968. Locus of the action of serum and the role of lysozyme in the serum bactericidal reaction. Journal of Bacteriology 96a:(6) 2118-2126.

10.
Khan, M. A., I. Hussain, M. Ashfaque and K. M. Ahmad. 1990. Studies on bacterial etiology of metritis with special reference to Brucella in buffaloes and cows. Pak. Vet. J. 10(4):157-158.

11.
Krishnan, R., S. K. Thanwan and M. N. Moghe. 1994. Antibiotic pattern of bacteria isolated from repeat breeding animals. Indian Vet. J. 71:315-317.

12.
Mathew, S. and C. Kuttan. 1997. Antioxidant activty of Tinospora cordifolia and its use in amelioration of cyclophosphamide toxicity. J. Exp. Clin. Cancer Res. 16:407-411.

13.
Myrvik, Q. N. 1956. Serum bactericidins active against grampositive bacteria. Ann. N.Y. Acad. Sci. 66:391-400. crossref(new window)

14.
Nguyen-van-Thanh 1997. Bacteria in genital tract of normal female buffaloes and of those with metritis in North Vietnam. Khoa-Hoc-ky-Thuat-Thu-Y, 4:40-43.

15.
Pateria, A. K., P. N. Kumar, C. V. S. Rawal. 1989. Studies on microbial involvement in metritis affected buffaloes. Ind. J. Comp. Micro. Immunol. Infect. Dis. 10(3):

16.
Popov, Yu N. 1969. Diagnosis of occult endometritis in cows (using white side test on cervical mucous). Veterinariya Moscow No. 4 pp. 85-87.

17.
Prince, P. S. and V. P. Menon. 1999. Antioxidant activity of Tinospora cordifolia roots in experimental diabetes. J. Ethnopharmacol, 63(3):277-281.

18.
Rakesh Sharda and Rajeev Krishnan 2001. Aerobic bacterial flora of cervical mucous of repeat breeder cattle and buffaloes. Indian Vet. J. 78:659-660.

19.
Rege, N. N. and S. A. Dahanukar. 1997. Quantitation of microbicidal activity of mononuclear phagocytes, an in vitro technique. J. Postgrad. Med. 39(1): 22-5.

20.
Rege, N. N., U. M. Thatte and S. A. Dahanukar. 1999. Adaptogenic properties of six Rasayana herbs used in Ayurvedic medicine. Phytotherapy, Res. 13(4):275-91. crossref(new window)

21.
Roberts, S. J. 1986. In: Veterinary obstetrics and genital diseases 3rd Edn. (Ed. S. J. Roberts). Woodstock Vermont pp. 538-546.

22.
Saini, P. S., A. S. Nanda, A. S. Grewal and J. Singh. 1999. Uterine defence modulation for the treatment of repeat breeding due to infectious endometritis in bovines. Indian J. Anim. Sci. 69:307-309.

23.
Sainis, K. B. 1997. Immunomodulation (Ed. S. N. Upadhyay), p. 155, Narora publishing house, New Delhi.

24.
Singh, S. N. 2000. Therapeutic management of bovine subclinical mastitis with special reference to medicinal herbs. M.V.Sc. thesis submitted to Deemed University, IVRI, Izatnagar, India.

25.
Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods, 8th edn. The Iowa State Univeristy Press, Ames, Iowa, USA.

26.
Sohni, Y. R., P. Kaimal and R. M. Bhatt. 1995. The antiamoebic effect of a crude drug formulation of herbal extracts against Entamoeba histolytica in vitro and in vivo. J. Ethnopharmacol. 45(1):43-52.

27.
Tarpstra, J. I. and W. A. Eisma. 1951. Sterility caused by bacteria Tijdschr, Diergeneck, 76:363-368 (c.f. Vet. Bull, 22:253).

28.
Taylor, P. W. 1987. Bactericidal and bacteriolytic activity of serum against G -ve bacteria. Microbiol. Rev. 47:46-83.

29.
Thatte, U. M. and S. A. Dahanukar. 1989. Phytotherapy Res. 3(2):43. Cited in product manual of Immumod (M/s Merind Ltd.)

30.
Venugopal, R. 1995. Intrauterine infusion of homologous plasma in the treatment of endometritis in cows. M.V.Sc. thesis submitted to college of veterinary and animal science, Mannuthy, Tissur.

31.
Waelchli, R. O., L. Corboz and N. C. Winder. 1987. Effect of intra-uterine plasma infusion in the mare: Histological, Bacteriological and cytological findings. 28(6):861-869.

32.
Ward, A. 1985. A new treatment for infertility in mares. Vet. Med. 80:65-69.

33.
Watson, E. D. 1988. Uterine defence mechanisms in mares resistant and succeptible to persistant endometritis: A review. Equine Vet. J. 20:397-400.