Advanced SearchSearch Tips
Genetic Variations Analysis and Characterization of the Fifth Intron of Porcine NRAMP1 Gene
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Genetic Variations Analysis and Characterization of the Fifth Intron of Porcine NRAMP1 Gene
Yan, X.M.; Ren, J.; Ai, H.S.; Ding, N.S.; Gao, J.; Guo, Y.M.; Chen, C.Y.; Ma, J.W.; Shu, Q.L.; Huang, L.S.;
  PDF(new window)
The natural resistance-associated macrophage protein 1 (NRAMP1) gene was identified as a candidate gene controlling the resistance and susceptibility to a number of intracellular parasites in pigs. The genetic variations in a 1.6 kb region spanning exon 1 and exon 3 of the porcine NRAMP1 gene were investigated by PCR-HinfI-RFLP in samples of 1347 individuals from 21 Chinese indigenous pig populations and 3 western pig breeds. Three alleles (A, B, C) and four genotypes (AA, BB, AB, BC) were detected. Significant differences in genotype and allele frequencies were observed between Chinese indigenous pig populations and exotic pig breeds, while in general the differences in genotype and allele frequencies among Chinese indigenous pig populations were not significant. The allele C was detected only in Duroc, Leping Spotted and Dongxiang Spotted pig, and the two Chinese pig populations showed similar genotype and allele frequencies. Four Chinese Tibetan pig populations displayed genetic differentiation at the NRAMP1 gene locus. In addition, intron 5 of the NRAMP1 gene was isolated and characterized by directly sequencing the PCR products encompassing intron 5. The alignment of intron 5 of the porcine, human, equine and ovine NRAMP1 gene showed a similarity of 45.38% between pig and human, 52.55% between pig and horse, 63.47% between pig and sheep, respectively
Pig;NRAMP1 Gene;Genetic Variation;Intron 5;
 Cited by
Bellamy, R. 1999. The natural resistance-associated macrophage protein and susceptibility to intracellular pathogens. Microbes and Infection.1: 23-27. crossref(new window)

Blackwell, J. M., S. Searle and T. Goswami. 2000. Understanding the multiple functions of NRAMP1. Microbes and Infection.2: 317-321.

Cellier, M., G. Govoni and S. Vidal. 1994. Human natural resistance-associated macrophage protein: cDNA cloning, chromosomal mapping, genomic organization, and tissuespecific expression. J. Exp. Med.180:1741-1752.

Forbes, J. R. and P. Gros. 2001. Divalent-metal transport by NRAMP proteins at the interface of host-pathogen interactions. Trends in Microbiology. 9:397-403.

He, L. 2000. Decoding life (In Chinese). Science Press, Beijing.

Lai, Y. B. and F. Y. Huang. 2001. Annals for native livestock and poultry breeds in Jiangxi Province (In Chinese). Jiangxi Science and Technology Press, Nanchang.

Malo, D. and E. Skamene. 1994. Genetic control of host resistance to infection. Trends in Genetics. 10:365-371.

SAS Institue Inc. 1989. SAS/STAT User's Guide: Version 6. 4th edn. SAS Institute Inc, Cary, North Carolina.

Strauss, W. M. 1991. Preparation of genomic DNA from mammalian tissue. In: Current Protocols in molecular Biology (Ed. F. M. Ausubel, R. Brent and R. E. Kingston) Vol. 1, John Wiley and Sons. New York, N.Y., USA. pp. 221-223.

Sun, H. S., L. Wang and M. F. Rothschild. 1998. Mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15. Anim. Genet. 29:138-140.

Tuggle, C. K., C. B. Schmitz and D. Gingerich-Feil. 1997. Rapid communication: cloning of a pig full-length natural resistanceassociated macrophage protein (NRAMP1) cDNA. J. Anim. Sci. 75:277.

Vidal, S. M., D. Malo and K. Vogan. 1993. Natural resistant to infection with intracellular parasites: isolation of a candidate for Bcg. Cell. 73:469-485.

Wang, W. J., L. S. Huang and K. F. Chen. 2002. Polymorphism of insulin-like growth factor-I gene in 13 pig breeds and its relationship with pig growth and carcass traits. Asian-Aust. J. Anim. Sci. 15:1391-1394.