JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Comparison of Gene Expression Patterns in Longissimus dorsi of Pigs between the High-parent Heterosis Cross Combination andrace×Large White and the Mid-parent Heterosis Cross Combination Large White×Meishan
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Comparison of Gene Expression Patterns in Longissimus dorsi of Pigs between the High-parent Heterosis Cross Combination andrace×Large White and the Mid-parent Heterosis Cross Combination Large White×Meishan
Liu, G.Y.; Xiong, Y.Z.; Deng, C.Y.; Zuo, B.; Zhang, J.H.;
  PDF(new window)
 Abstract
In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences in gene expression of pig's Longissimus dorsi between the high-parent heterosis cross combination LandraceLarge White and the mid-parent heterosis cross combination Large WhiteMeishan. Three pig purebreds, Large White, Meishan, and Landrace and four types of reciprocal hybrids were analyzed using nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers and nearly 7,000 reproducible bands were examined. The patterns of gene expression of each cross combination were analyzed and eight common patterns (fifteen kinds) were found. When the results from the two cross combinations were put together and compared, eight different typical expression patterns were observed, these indicated that the patterns of gene expression of these two cross combinations had obvious differences. Gene expression correlation and cluster analyses of the two cross combinations indicated that the gene expression of the mid-parent heterosis cross combination was correlated with maternal effect, but in the high-parent heterosis cross combination, paternal effect acted in the gene expression of the hybrids or the gene expression of the hybrids was biased towards one parent.
 Keywords
Differential Display;Heterosis;Gene Expression Patterns;Correlation and Cluster Analyses;Maternal Effect;Paternal Effect;
 Language
English
 Cited by
1.
Molecular characterization and expression profile of a novel porcine gene differentially expressed in the muscle tissues from Meishan, Large White and their hybrids, Molecular Biology Reports, 2009, 36, 1, 57  crossref(new windwow)
2.
Differential display reveals a novel pig gene, PRPF3, which is differentially expressed in Large White versus Wujin skeletal muscle tissues, Molecular Biology Reports, 2010, 37, 6, 2687  crossref(new windwow)
3.
Identification of a differential gene HUMMLC2B between F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, Gene, 2005, 352, 118  crossref(new windwow)
4.
Molecular cloning and expression analyses of a novel swine gene-ARF4, Molecular Biology Reports, 2009, 36, 3, 455  crossref(new windwow)
5.
Molecular cloning, sequence identification and expression profile of domestic guinea pig (Cavia porcellus) UGT1A1 gene, Biotechnology & Biotechnological Equipment, 2016, 30, 1, 106  crossref(new windwow)
6.
A novel porcine gene, LIPC, differentially expressed in the liver tissues from Meishan and Large White pigs, Journal of Applied Genetics, 2011, 52, 2, 219  crossref(new windwow)
7.
Molecular characterization of the encoding regions and tissue expression analyses for three novel porcine genes––HNRPA1, YIPF5 and UB2D2, Molecular Biology Reports, 2008, 35, 4, 519  crossref(new windwow)
8.
Molecular characterization and expression profile of a novel porcine gene differentially expressed in the muscle and backfat tissues from Chinese Meishan and Russian Large White pigs, Molecular Biology, 2008, 42, 4, 499  crossref(new windwow)
9.
Cloning, sequence characterization, and tissue expression profile analysis of three novel porcine genes—RHOB, RHOG, and PRAF1, Molecular Biology, 2008, 42, 1, 52  crossref(new windwow)
10.
A Novel Porcine Gene-erlin2, Differentially Expressed in the Liver Tissues from Meishan and Large White Pigs, Applied Biochemistry and Biotechnology, 2010, 160, 4, 1047  crossref(new windwow)
11.
Analysis of Genetic Diversity and Relationships of Seven Chinese Indigenous Pig Breeds and Three Exotic Pig Breeds Using the DNA Differential Display Technique, Agricultural Sciences in China, 2006, 5, 9, 713  crossref(new windwow)
12.
Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from black-boned sheep (Ovis aries), Molecular Biology, 2010, 44, 1, 14  crossref(new windwow)
13.
Isolation, sequence identification and tissue expression profiles of 3 novel porcine genes:ASPA, NAGA, andHEXA, Journal of Applied Genetics, 2008, 49, 3, 257  crossref(new windwow)
14.
A novel sheep gene,MMP7, differentially expressed in muscles from black-boned sheep and local common sheep, Journal of Applied Genetics, 2009, 50, 3, 253  crossref(new windwow)
15.
Isolation, sequence analysis and expression profile of a novel porcine gene,CXCL10, differentially expressed in the Longissimus dorsi muscle tissues from Meishan, Meishan × Large White cross and Large White pigs, DNA Sequence, 2007, 18, 6, 415  crossref(new windwow)
16.
Isolation, Sequence Analysis and Expression Profile of a Novel Swine Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from LandracexLarge White Cross-combination, Acta Biochimica et Biophysica Sinica, 2005, 37, 3, 186  crossref(new windwow)
17.
Association of Single Nucleotide Polymorphisms in Exon 3 of Porcine LMCD1 Gene with Meat Quality and Carcass Traits, Agricultural Sciences in China, 2008, 7, 3, 370  crossref(new windwow)
18.
Isolation, sequence identification and tissue expression distribution of three novel porcine genes—RAB14, S35A3 and ITM2A, Molecular Biology Reports, 2008, 35, 2, 201  crossref(new windwow)
19.
A novel porcine gene, MAPKAPK3, is differentially expressed in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs, Molecular Biology Reports, 2010, 37, 7, 3345  crossref(new windwow)
20.
Molecular cloning, polymorphism and association analyses of a novel differentially expressed porcine mRNA, Molecular Biology Reports, 2009, 36, 8, 2279  crossref(new windwow)
21.
cDNA cloning, sequence identification and tissue expression distribution of three novel porcine genes: UCHL3, RIT1 and CCND3, Molecular Biology Reports, 2009, 36, 3, 521  crossref(new windwow)
22.
A novel porcine gene, POT1, differentially expressed in the longissimus muscle tissues from Wujin and Large White pigs, Cytokine, 2012, 59, 1, 22  crossref(new windwow)
23.
Isolation, nucleotide identification and tissue expression of three novel ovine genes—SLC25A4, SLC25A5 and SLC25A6, Molecular Biology Reports, 2010, 37, 6, 2743  crossref(new windwow)
24.
A porcine gene, PBK, differentially expressed in the longissimus muscle from Meishan and Large White pig, Genetics and Molecular Biology, 2009, 32, 4, 771  crossref(new windwow)
25.
Isolation, sequence identification and tissue expression profile of three novel porcine genes –ARL1,ARL3andARL4A, Acta Agriculturae Scandinavica, Section A - Animal Science, 2007, 57, 1, 10  crossref(new windwow)
26.
Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1, Molecular Biology Reports, 2009, 36, 4, 683  crossref(new windwow)
27.
Isolation, sequence identification, and tissue expression profile of 3 novel porcine genes:NCF2, BCKDHB andBCKDHA, Journal of Applied Genetics, 2009, 50, 1, 47  crossref(new windwow)
28.
Isolation, sequence analysis and expression profile of a novel porcine gene, NIP7, differentially expressed in the Longissimus dorsi muscle tissues from Meishan, Meishan × Large White cross and Large White pigs, Molecular Biology Reports, 2007, 34, 4, 213  crossref(new windwow)
29.
Molecular cloning, polymorphism and association analyses of a novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs, Molecular Biology Reports, 2009, 36, 6, 1393  crossref(new windwow)
 References
1.
Davenport, C. B. 1908. Degeneration, albinism and inbreeding. Science. 28:454-455.

2.
East, E. M. 1908. Report of the Connecticut Agricultural Experimental Station for Years 1907-1908. 419-428.

3.
Hua, J., Y. Xing, W. Wu, C. Xu, X. Sun, S. Yu, Q. Zhang. 2003. Single-locus heterotic effects and dominance by dominance interactions can adequately explain the genetic basis of heterosis in an elite rice hybrid. Proc. Natl. Acad. Sci. USA. 100: 2574-2579.

4.
Liang, P. and A. B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-971.

5.
Liang, P., L. Averboukh and A. B. Pardee. 1993. Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res. 21:3269-3275.

6.
Linskens, M. H. 1995. Cataloging altered gene expression in young and senescent cells using enhanced differential display. Nuclei Acids Res. 23:3244-3251. crossref(new window)

7.
Lu, H., J. Romero-Severson and R. Bernardo. 2003. Genetic basis of heterosis explored by simple sequence repeat markers in a random-mated maize population. Theor. Appl. Genet. 107:494-502.

8.
Pan, Peiwen. 2002. Isolation, characterization and mapping of differentially expressed EST from porcine skeletal in different breeds or developing stages. Ph.D. Thesis, Huazhong Agricultural University, Wuhan, China.

9.
Pan, P. W., S. H. Zhao, M. Yu, T. A. Xiong and K. Li. 2003. Identification of Differentially Expressed Genes in the Longissimus Dorsi Muscle Tissue between Duroc and Erhualian Pigs by mRNA Differential Display. Asian-Aust. J. Sci. Vol 16, No.7:1066-1070.

10.
Shull, G. H. 1908. The composition of a field of maize. American Breeders' Association Report 4:296-301.

11.
Wu, L. M., Z. F. Ni, Z. K. Wang, Z. Lin and Q. X. Sun. 2001. Relationship between differential expression patterns of multigene families and heterosis in a wheat diallel crosses. Yi Chuan Xue Bao. 28:256-266.

12.
Xiao, J., J. Li, L. Yuan and S. D. Tanksley. 1995. Dominance as the major genetic basis of heterosis in rice. Proceedings of the Third International Rice Genetics Symposium, Manila (Philippines).

13.
Xie, X. D., Z. F. Ni, F. R. Meng, L. M. Wu, Z. K. Wang and X. Sun. 2003. Relationship between differences of gene expression in early developing seeds of hybrid versus parents and heterosis in wheat. Yi Chuan Xue Bao. 30:260-266.

14.
Yamazaki, M. and K. Saito. 2002. Differential display analysis of gene expression in plants. CMLS, Cell. Mol. Life Sci. 59:1246-1255.

15.
Yu, S. B., J. X. Li, C. G. Xu, Y. F. Tan, Y. J. Gao, X. H. Li, Qifa Zhang and M. A. Saghai Maroof. 1997. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. Genetics. 94: 9226-9231.