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Molecular Characterization of Rathi and Tharparkar Indigenous Cattle (Bos indicus) Breeds by RAPD-PCR
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 Title & Authors
Molecular Characterization of Rathi and Tharparkar Indigenous Cattle (Bos indicus) Breeds by RAPD-PCR
Sharma, Amit Kumar; Bhushan, Bharat; Kumar, Sanjeev; Kumar, Pushpendra; Sharma, Arjava; Kumar, Satish;
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Random amplification of polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out using DNA samples of 30 animals of Rathi cattle and 42 animals of Tharparkar cattle. Genomic DNA was isolated as per standard protocol and evaluated for its quality, purity and concentration. Twenty three random primers were screened out of which 15 primers yielded satisfactory amplifications and were used for further analysis. Average numbers of polymorphic fragments per primer were 7.070.86 in Rathi and 6.800.61 in Tharparkar cattle. The percentage of polymorphic bands in these two cattle breeds were 86 and 87%, respectively. Within breed genetic similarities for pooled over primers in the animals of Rathi and Tharparkar breeds were .5770.30 and 0.5310.02, respectively on the basis of band frequency (BF) and 0.6450.04 and 0.5340.04, respectively on the basis of band sharing (BS). Averages of between breed genetic similarities for pooled over primers were 0.97 and 0.92 according to BF and BS, respectively, which reflect higher degree of genetic similarity between Rathi and Tharparkar cattle breeds. Index of genetic distance based on BF and BS for pooled over primers was 0.0300.011 and 0.0880.031, respectively. Percentage of polymorphic bands and within-breed genetic similarities on the basis of band frequency (BF) and band sharing (BS) for pooled over primers revealed higher genetic similarity in Rathi than Tharparkar cattle population. High estimates of between breed genetic similarities for pooled over primers indicated that either Rathi is having decent from Tharparkar or both the cattle breeds are having common descent. Low value of Index of genetic distances between these two cattle breeds may be due to the fact that Rathi and Tharparkar cattle breeds are the native of Thar Desert in Northwest India. The results of between breed genetic distances also confirm the existence of high degree of genetic similarity between these two breeds of cattle.
RAPD-PCR;Molecular Characterization;Rathi and Tharparkar Cattle;
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Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 9, pp.1233-1237 crossref(new window)
Anderson, L., J. Bohme, L. Rask and P. A. Peterson. 1986. Genomic Hybridization of bovine class II major histocompatibility genes: 1. Extensive polymorphism of DQ$\alpha$ and DQ$\beta$ genes. Anim. Genet. 17:95-112.

Apuya, N. R., B. L. Frazier, P. Keim, E. J. Roth and K. G. Lark. 1988. Restriction fragment length polymorphism as genetic markers in Soya bean Glycine max (L.) Merrill. Theor. Appl. Genet. 75:889-901.

Baird, E., S. C. Bland, R. Waugh, M. D. Maine and W. Powell. 1992. Molecular characterization of inter and intro specific somatic hybrids of potato using random amplified polymorphic DNA (RAPD) marker. Molecular and General Genet. 223(3):469-475.

Bardin, M. G., C. Bandi, S. Comincini, G. Damiani and P. Rognoni. 1992. Use of RAPDs marker to estimate genetic variation in bovine populations. Anim. Genet. 23:57.

Bhattacharjya, T. K. 1999. Genetic characterization of Indigenous breeds of cattle by PCR based methods. Ph.D. thesis, Deemed University IVRI, Izatnagar, Barilly (India). p. 88.

Bowditch, B. M., D. G. Albright, J. G. K. Willians and M. J. Braun. 1993. Use of randomly amplified polymorphic DNA marker in comparative genome studies. Methods in Enzymology, 224:294-309.

Chapaco, W., N. W. Ashton, R. K. Martel, N. Antonishyn and W. L. Crosby. 1992. A feasibility study of use of random amplified polymorphic DNA in the population genetic and systematic of grass hopers. Genome, 35:569-575.

Cushwa, W. T., K. G. Dodds, A. M. Crawferd and J. F. Medrano. 1996. Identification and genetic mapping of random amplified polymorphic DNA (RAPD) markers to the sheep genome. Mamm. Genome, 7:550-585.

Dunnington, E. A., Y. Plotsky, A. Haberfeld, T. Kirk, A. Goldberg, Y. Lavi, A. Chaner, P. B. Siegel and J. Hillel. 1990. DNA fingerprints of chickens selected for high and low body weight for 31 generation. Anim. Genet. 21:247-257.

Gwakisa, P. S., S. J. Kemp and A. J. Teale. 1994. Characterization of Zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. Anim. Genet. 25:89-94.

Jeffreys, A. J. and D. B. Morton. 1987. DNA fingerprinting of dogs and cats. Anim. Genet. 18:1-15.

Kantanen, J., J. Vikki, K. Elo and T. Maki. 1995. Random amplified polymorphic DNA in cattle and sheep: amplification for detecting genetic variation. Anim. Genet. 26:315-320.

Kemp, S. J. and A. J. Teale. 1992. Random amplified DNA polymorphism (RAPDs) and pooled DNA bovine genetic study. Anim. Genet. 23:62.

Lynch, M. 1990. The similarity index and DNA fingerprinting. Mol. Biol. Evol. 7:478-484.

Michelmore, R. W., I. Paran and R. V. Kesseli. 1991. Identification of markers in specific genomic regions by using segregating populations. Proceeding of the Natural Academic Science, USA. Vol. 88:9828-9832.

Mittal, A. P., S. Prasad and C. J. C. Phillips. 1989. Rathi a new breed of cattle from the Indian desert. New technique in cattle production, 2:244-246.

Ramesha, K. P., T. Saravanan, M. K. Rao, M. M. Appannavar and A. O. Reddy. 2002. Genetic distance among south Indian breeds of zebu cattle using random amplified DNA markers. Asian-Aust. J. Anim. Sci. 15(3):309-314.

Wahid, A. 1971. Draft monograph on Thari cattle. Mimeograph, Karachi University.

Welsh, J. and M. McClelland. 1991. Genomic finger printing using arbitrarily prime PCR and a matrix of pair wise combinations of primers. Nucleic Acid Res. 19:5275-5279. crossref(new window)

Wetton, J. H., R. E. Carter, D. T. Parkin and Walters. 1987. Demographic study of a wild hours sparrow population by DNA fingerprinting. Nature, 327:147-148.

Yu, K. F. and K. P. Pauls. 1992. Optimization of the PCR programme for RAPD analysis. Nucleic acid Research 20:2606.