Advanced SearchSearch Tips
Effects of Halogenated Compounds, Organic Acids and Unsaturated Fatty Acids on In vitro Methane Production and Fermentation Characteristics
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Halogenated Compounds, Organic Acids and Unsaturated Fatty Acids on In vitro Methane Production and Fermentation Characteristics
Choi, N.J.; Lee, S.Y.; Sung, H.G.; Lee, S.C.; Ha, J.K.;
  PDF(new window)
The objective of this study was to evaluate the effects of halogenated compounds, organic acids, unsaturated fatty acids and their mixtures on in vitro methane production and fermentative characteristics of mixed rumen microorganisms. Agents used in two in vitro experiments were bromoethanesulfonic acid (BES) and pyromellitic diimide (PMDI) as halogenated compound, fumarate and malate as organic acid, and linoleic acid and linolenic acid as unsaturated fatty acid sources. Ruminal fluid collected from a Holstein steer fed tall fescue and concentrate mixtures was incubated at for 48 h with addition of those materials. Single supplementation of halogenated compounds, organic acids or unsaturated fatty acids decreased in vitro methane production (p<0.05). The second experiment was designed to investigate effects of combination of one of halogenated compounds and either organic acids or fatty acids on methane production. Lower concentration of methane and lower A:P ratio were observed with PMDI compared with BES (p<0.01). In general medium pH, VFA, total gas and hydrogen production, and dry matter degradability were affected by addition of the same compounds. In addition, PMDI+malate treatment resulted in the highest molar proportion of propionate, and lowest A:P ratio and methane production (p<0.01). Hydrogen production was highest in PMDI+linolenic acid and lowest in BES+malate treatment (p<0.01). PMDI+malate combination was the most recommendable in reducing methane production without too much influence on digestibility under conditions of present studies.
Methane;Fermentation;Halogenated Compounds;Organic Acids;Unsaturated Fatty Acids;
 Cited by
Control of Methane Emission in Ruminants and Industrial Application of Biogas from Livestock Manure in Korea,Song, Man-K.;Li, Xiang-Z.;Oh, Young-K.;Lee, Chang-Kyu;Hyun, Y.;

Asian-Australasian Journal of Animal Sciences, 2011. vol.24. 1, pp.130-136 crossref(new window)
In vitro Methanogenesis, Microbial Profile and Fermentation of Green Forages with Buffalo Rumen Liquor as Influenced by 2-Bromoethanesulphonic Acid,Agarwal, Neeta;Kamra, D.N.;Chatterjee, P.N.;Kumar, Ravindra;Chaudhary, L.C.;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 6, pp.818-823 crossref(new window)
Asanuma, N., M. Iwamoto and T. Hino. 1999. Effect of the addition of fumarate on methane production by ruminal microorganisms in vitro. J. Dairy Sci. 82:780-787.

Callaway, T. R. and S. A. Martin. 1996. Effects of organic acid and monensin treatment on in vitro mixed ruminal microorganism fermentation of cracked corn. J. Anim. Sci. 74:1982-1989.

Carro, M. D. and M. J. Ranilla. 2003. Influence of different concentrations of disodium fumarate on methane production and fermentation of concentrate feeds by rumen microorganisms in vitro. Br. J. Nutr. 90:617-623.

Chalupa, W., B. Vecchiarelli, A. E. Elser, D. S. Kronfeld, D. Sklan and D. L. Palmquist. 1984. Rumen fermentation in vitro as influenced by long chain fatty acids. J. Dairy Sci. 67:1439-1444.

Crutzen, P. J., I. Aselmann and W. Seiler. 1986. Methane production by domestic animals, wild ruminants, other herbivorous fauna and humans. Tellus. 388:271-284.

Czerkawski, J. W., K. L. Blaxter and F. W. Wainman. 1966. The metabolism of oleic, linoleic, and linolenic acids by sheep with reference to their effects on methane production. Br. J. Nutr. 20:349-362.

Czerkawski, J. W. 1986. An Introduction to Rumen Studies. New York, Pergamon Press.

Demeyer, D. I. and C. J. Van Nevel. 1975. Methanogenesis, an integrated part of carbohydrate fermentation, and its control. In Digestion and Metabolism in the Ruminant (Ed. I. W. McDonald and A. C. I. Warner) pp. 366-382. The University of New England Publishing Unit. Armidale, N. S. W., Australia.

Demeyer, D. I., C. J. Van Nevel, H. K. Henderickx and J. Martin. 1969. The effect of unsaturated fatty acids upon methane and propionic acid in the rumen. In: Energy Metabolism of Farm Animals (Ed. K. L. Blaxter, J. Kielanowski and G. Thorbek) pp. 139-147. Oriel Press, Newcastle upon Tyne, UK.

Erwin, E. S., G. J. Marco and E. M. Emery. 1961. Volatile fatty acid analysis of blood and rumen fluid by gas chromatography. J. Dairy Sci. 41:1768-1770.

Hino, T. and J. B. Russell. 1985. Effect of reducing-equivalent disposal and NADH/NAD on deamination of amino acids by intact rumen microorganisms and their cell extracts. Appl. Environ. Microbiol. 50:1368-1374.

Hino, T. and Y. Nagatake. 1993. The effects of grass lipids on fibre digestion by mixed rumen microorganisms in vitro. Anim. Sci. Technol. 64:121-128.

Hungate, R. E. 1969. A roll tube method for cultivation of strict anaerobes. In: Methods in Microbiology (Ed. J. R. Norris and D. W. Ribbons) pp. 117-132. Academic Press, New York, USA.

Hungate, R. E., W. Smith, T. Bauchop, I. Yu and J. C. Rabinowitz. 1970. Formate as an intermediate in the bovine rumen fermentation. J. Bacteriol. 102:389-397.

Lennarz, W. J. 1966. Lipid metabolism in the bacteria. Adv. Lipid Res. 4:175-225.

Lee, H. J., S. C. Lee, J. D. Kim, Y. G. Oh, B. K. Kim, C. W. Kim and K. J. Kim. 2003. Methane production potential of feed ingredients as measured by in vitro gas test. Asian-Aust. J. Anim. Sci. 16:1143-1150.

Lila, Z. A., N. Mohammed, S. Kanda, T. Kamada and H. Itabashi. 2003. Effect of sarsaponin on ruminal fermentation with particular reference to methane production in vitro. J. Dairy Sci. 86:3330-3336.

Martin, S. A. 1998. Manipulation of ruminal fermentation with organic acids: a review. J. Anim. Sci. 76:3123-3132.

Martin, S. A. and J. M. Macy. 1985. Effects of monensin, pyromellitic diimide and 2-bromoethanesulfonic acid on rumen fermentation in vitro. J. Anim. Sci. 60:544-550.

Martin, S. A. and M. N. Streeter. 1995. Effect of malate on in vitro mixed ruminal microorganism fermentation. J. Anim. Sci. 73:2141-2145.

McDougall, E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheep’s saliva. Biochem. J. 43:99-109.

Moss, A. R. 1993. Methane: global warming and production by animals. Chalcombe Publications, Kingston, UK.

Russell, J. B. and H. J. Strobel. 1989. Effect of ionophores on ruminal fermentation. Appl. Environ. Microbiol. 55: 1-6.

Santoso, B., S. Kume, K. Nonaka, K. Kimura, H. Mizukoshi, Y. Gamo and J. Takahashi. 2003. Methane emission, nutrient digestibility, energy metabolism and blood metabolites in dairy cows fed silages with and without galacto-oligosaccharides supplementation. Asian-Aust. J. Anim. Sci. 16:534-540.

SAS. 1999. SAS user's guide: Statistics (Version 8.01 Ed.). SAS Inst. Inc., Cary, N.C. USA.

Snedecor, G. W. and W. G. Cochran. 1967. Statistical Methods (6th Ed.). Iowa State Univ. Press, Ames.

Takahashi, J. 2001. Nutritional manipulation of methanogenesis in ruminants. Asian-Aust. J. Anim. Sci. 14:131-135.

Theodorou, M. K., B. A. Williams, M. S. Dhanoa, A. B. McAllan and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feedstuffs. Anim. Feed Sci. Technol. 48:185-197.

Thomson, D. J. 1972. Physical form of the diet in relation to rumen fermentation. Proc. Nutr. Soc. 31:127-139.

Van Nevel, C. J. and D. I. Demeyer. 1988. Manipulation of rumen fermentation. In The rumen microbial ecosystem (Ed. P. N. Hobson) pp. 387-443. Elsevier Science Publishers, New York, USA.

Van Nevel, C. J. and D. I. Demeyer. 1995. Feed additives and other interventions for decreasing methane emissions. In Biotechnology in animal feeds and animal feeding (Ed. R. J. Wallace and A. Chesson) pp. 329-349. VCH, Weinheim, Germany.

Zinn, R. A. 1989. Influence of level and source of dietary fat on its comparative feeding value in finishing diets for feedlot steers: metabolism. J. Anim. Sci. 67:1038-1049.