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Potential Health Benefits of Conjugated Linoleic Acid (CLA): A Review
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Potential Health Benefits of Conjugated Linoleic Acid (CLA): A Review
Khanal, R.C.;
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Conjugated linoleic acid (CLA) is a mixture of positional and geometric isomers of octadecadienoic acid with two conjugated double bonds. Of more than a dozen isomers of CLA found naturally in dairy and meat products from ruminants, c-9, t-11 and t-10, c-12 are the two isomers with known physiological importance, including anticarcinogenic, antidiabetic, antilipogenic, and antiatherosclerotic effects. Positive effects of CLA on immune function and bone modeling have also been reported. In spite of the compelling findings in tissue cultures and experimental animal models, its effect, dose, and mechanism of action vis-à-vis specific isomers remains speculative. Results obtained from animal models are inconclusive and conflicting at times in humans, where the research data is limited. It appears that there is a long way to go before CLA could be accepted unequivocally as having definite effects in any or all of these physiological states and how such effects actually occur in humans. The objective of this review is to critically examine the available literature on potential health benefits of CLA observed in cell culture, animal models, and human subjects, wherever possible and to a certain extent the mechanism of action associated with these biological activities.
Conjugated Linoleic Acid;Potential Health Benefits;Animals;Humans;
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Albers, R., R. P. van der Wielen, E. J. Brink, H. F. Hendricks, V. N. Taran-Dorovska and I. C. Mohede. 2003. Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid (CLA) isomers on immune function in healthy men. Eur. J. Clin. Nutr. 57:595-603.

An, B. K., K. H. Shinn, Y. Kobayashi, N. Tanaka and C. W. Kang. 2003. Excessive dietary conjugated linoleic acid affects hepatic lipid content and muscular fatty acid composition in young chicks. Asian-Aust. J. Anim. Sci. 16:1171-1176.

Aro, A. S. Mannisto, I. Salminen, M.L. Ovaskainen, V. Kataja and M. Uusitupa. 2000. Inverse association between dietary and serum conjugated linoleic acid and risk of breast cancer in postmenopausal women. Nutr. Cancer. 38:151-157.

Azain, M. J., D. B. Hausman, M. B. Sisk, W. P. Flatt and D. E. Jewell. 2000. Dietary conjugated linoleic acid reduces rat adipose tissue cell size rather than cell number. J. Nutr. 130:1548-1554.

Banni, S., C. S. D. Heys and K. W. J. Wahle. 2003. Conjugated linoleic acid as anticancer nutrients: Studies in vivo and cellular mechanisms. In: Advances in Conjugated Linoleic Acid Research. Vol. 2. (Ed. J. Sebedio, W. W. Christie and R. Adolf). AOCS Press, Champaign, IL, pp. 267-281.

Banni, S., E. Angioni, G. Carta, V. Casu, M. Deiena, M. A. Dessi, L. Lucchi, M. P. Melis, A. Rosa, S. Vargiolu and F. P. Corongiu. 1999. Influence of dietary conjugated linoleic acid on lipid metabolism in relation to its anticarcinogenic activity. In: (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, M. W. Pariza and G. J. Nelson). Advances in Conjugated Linoleic Acid Research. Vol. I. AOCS Press, Champaign, IL, pp. 307-318.

Bassaganaya-Riera, B., R. Hontecillas-Magarzo, K. Bregendahl, M. J. Wannenmuehler and D. R. Zimmerman. 2001. Effects of dietary conjugated linoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. J. Anim. Sci. 79:714-721.

Bassaganaya-Riera, J., R. M. Pogranichniy, S. C. Jobgen, P. G. Halbur, K. J. Yoon, M. O’Shea, I. Mochede and R. Hontecillas. 2003. Conjugated linoleic acid ameliorates viral infectivity in a pig model of virally induced immunosuppression. J. Nutr. 133:3204-3214.

Baumgard, L. H., B. A. Corl, D. A. Dwyer, A. S$S{\ae}b{\o}$ and D. E. Bauman. 2001. Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis. Am. J. Physiol. 278:R179-R184.

Baumgard, L. H., E. Matitasvili, B. A. Corl, D.A. Dwyer and D. E. Bauman. 2002. Trans-10, cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis. J. Dairy Sci. 85:2155-2163.

Bee, G. 2000a. Dietary conjugated linoleic acid consumption alter adipose tissue and milk lipids of pregnant and lactating sows. J. Nutr. 130:2292-2298.

Bee, G. 2000b. Dietary conjugated linoleic acid consumption during pregnancy and lactation influences growth and tissue composition in weaned pigs. J. Nutr. 130:2981-2989.

Belury, M. 2002a. Dietary conjugated linoleic acid in health: Physiological effects and mechanisms of action. Annu. Rev. Nutr. 22:505-531.

Belury, M. A. 2002b. Inhibition of carcinogenesis by conjugated linoleic acid: potential mechanisms of action. J. Nutr. 132:2995-2998.

Belury, M. A. 2003. Conjugated linoleic acids in type 2 diabetes mellitus: implications and potential mechanisms. In: Advances in Conjugated Linoleic Acid Research. Vol. 2. (Ed. J. Sebedio, W. W. Christie and R. Adolf). AOCS Press, Champaign, IL, pp. 302-315.

Belury, M. A. and J. P. Vanden Huevel. 1999. Modulation of diabetes by conjugated linoleic acid. In: (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, M. W. Pariza and G. J. Nelson). Advances in Conjugated Linoleic Acid Research. Vol. I. AOCS Press, Champaign, IL, pp. 404-411.

Belury, M. A., A. Mahon and S. Banni. 2003. The conjugated linoleic acid (CLA) isomer, t10c12-CLA, is inversely associated with changes in body weight and serum leptin in subjects with type 2 diabetes mellitus. J. Nutr. 133:257S-260S.

Belury, M. A., K. P. Nickel, C. E. Bird and Y. Wu. 1996. Dietary conjugated linoleic acid modulation of phorbol ester skin tumor promotion. Nutr. Cancer. 26:149-157.

Belury, M. A., S. Y. Moya-Camarena, M. Lu, L. Shi, L. M. Leesnitzer and S. V. Blanchard. 2002. Conjugated linoleic acid is an activator and ligand for peroxisome proliferator-activated receptor-gamma ($PPAR{\gamma}$) Nutr. Res. 22:817-824.

Benito, P., G. Nelson, D. Kelley, G. Bartolini, P. Schmidt and V. Simon. 2001. The effect of conjugated linoleic acid on plasma lipoproteins and tissue fatty acid composition in humans. Lipids. 36:229-236.

Bergano, P., D. Luongo and M. Rossi. 2004. Conjugated linoleic acid-mediated apoptosis in Jurkat T cells involves the production of reactive oxygen species. Cell Physiol. Biochem. 14:57-64.

Blankson, H., J. A. Stakkestad, H. Fagertun, E. Thom, J. Wadstein and O. Gudmundsen. 2000. Conjugated linoleic acid reduces body fat mass in overweight and obese humans. J. Nutr. 130:2943-2948.

Brown, J. M. and M. K. McIntosh. 2003. Conjugated linoleic acid in humans: regulation of adipocity and insulin sensitivity. J. Nutr. 133:3041-3046.

Brown, J. M., M. S. Boysen, S. S. Jensen, R. F. Morrison, J. Storkson, R. Lea-Currie, M. Pariza, S. Mandrup and M. K. McIntosh. 2003. Isomer-specific regulation of metabolism and $PPAR{\gamma}$ signaling by CLA in human preadipocytes. J. Lipid Res. 44:1287-1300.

Brown, J. M., Y. D. Halvorsen, Y. R. Lea-Currie, C. Geigerman and M. McIntosh. 2001. Trans-10 cis-12, but not cis-9, trans- 11, conjugated linoleic acid attenuates lipogenesis in primary cultures of stromal vascular cells from human adipose tissue. J. Nutr. 131:2316-2321.

Carta, G. E. Angioni, E. Murru, M. P. Melis, S. Spada and S. Banni. 2002. Modulation of lipid metabolism and vitamin A by conjugated linoleic acid. Prostaglandins Leukot. Essent. Fatty Acids. 67:187-191.

Cassone Faldetta, M., O. Laurenti, G. Desideri, M.c. Bravi, O. De Luca, M. C. Marinucci, G. De Mattia and C. Ferri. 2002. Larginine infusion decreases plasma total homocysteine concentrations through increased nitric oxide production amd decreased oxidative status in Type II diabetic patients. Diabetologia. 45:1120-1127.

Chajes, V., F. Lavillonniere, V. Maillard, B. Giraudeau, M. L. Jourdan, J. L. Sebedio and P. Bougnoux. 2003. Conjugated linoleic acid content in breast adipose tissue of breast cancer patients and the risk of metastasis. Nutr. Cancer 45:17-23.

Chardigny, J. M., O. Hasslewander, M. Genty, K. Kraemer, A. Ptock and J. L. Sebedio. 2003. Effect of conjugated linoleic acid on feed intake, body composition, and liver FA in mice. Lipids. 38:895-902.

Chen, B. Q., Y. B. Xue, J. R. Liu, Y. M. Yang, Y. M. Zheng, X. L. Wang and R. H. Liu. 2003. Inhibition of conjugated linoleic acid on mouse forestomach neoplasia induced by benzo (a) pyrene and chemopreventive mechanisms. World J. Gastroenterol 9:44-49.

Cheng, J. L., M. Futakuchi, K. Ogawa, T. Iwata, M. Kasai, S. Tokudome, M. Hirose and T. Shirai. 2003. Dose response study of conjugated fatty acid derived from safflower oil on mammary and colon carcinogenesis pretreated with 7,12-dimethylbenz[a]anthracene (DMBA) and 1, 2-dimethylhydrazine (DMH) in female Sprage-Dawley rats. Cancer Lett. 10:161-168.

Cheng, W. L., C. K. Lii, H. W. Chen, T. H. Lin and K. L. Liu. 2004. Contribution of conjugated linoleic acid to the suppression of inflammatory responses through the regulation of the NFkappaB pathway. J. Agric. Food Chem. 52:71-78.

Cho, H. J., W. K. Kim, E. J. Kim, K. C. Jung, S. Park, H. S. Lee, A. L. Tyner and J. H. Park. 2003a. Conjugated linoleic acid inhibits cell proliferation and ErbB3 signaling in HT-29 human colon cell line. Am. J. Physiol. Gastrointest. Liver physiol. 284:G996-G1005.

Cho, H. J., H. S. Lee, C. K. Chung, Y. H. Kang, Y. L. Ha, H. S. Park and J. H. Park. 2003b. Trans-10, cis-12- conjugated linoleic acid reduces insulin-like growth factor-II secretion in HT-29 human colon cancer cells. J. Med. Food. 6:193-199.

Choi, Y., Y. Park, M. W. Pariza and J. M. Ntambi. 2000. Regualtion of stearoyl-coenzyme A desaturase activity by the trans-10, cis-12, isomer of conjugated linoleic acid in HepG2 cells. Biochem. Biophys. Res. Commun. 284:689-693.

Chujo, H., M. Yamasaki, S. Nou, N. Koyanagi, H. Tachibana and K. Yamada. 2003. Effect of conjugated linoleic acid isomers on growth factor-induced proliferation of human breast cancer cells. Cancer Lett. 202:81-87.

Clement, L., H. Poirier, I. Niot, V. Bocjer, M. Guerro-Millo, S. Krief, B. Staels and P. Besnard. 2002. Dietary trans-10, cis-12 conjugated linoleic acidinduces hyperinsulinemia and fatty liver in the mouse. J. Lipid Res. 43:1400-1409.

Cook, M. E., D. Butz, G. Li, M. Pariza, L. Whigham and M. Yang. 2003. Conjugated linoleic acid enhances immune responses but protects against the collateral damage of immune events. In Advances in Conjugated Linoleic Acid Research. Vol. 2. (Ed. J. Sebedio, W. W. Christie and R. Adolf). AOCS Press, Champaign, IL, pp. 283-291.

Cook, M. E., D. DeVoney, B. Drake, M. W. Pariza, L. Whigham and M. Yang. 1999. Dietary control of immune induced cachexia: conjugated linoleic acid and immunity. In: (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, M. W. Pariza and G. J. Nelson). Advances in Conjugated Linoleic Acid Research. Vol. I. AOCS Press, Champaign, IL, pp. 226-237.

Corl, B.A., L.H. Baumgard, D.A. Dwyer, J. M. Griinari, B. S. Phillips and D. E. Bauman. 2001. The role of delta (9)- desaturase in the production of cis-9, trans-11 CLA. J. Nutr. Biochem. 12:622-630.

Corl, B. A., D. M. Barbano, D. E. Bauman and C. Ip. 2003. cis-9, trans-11 CLA derived endogenously from trans-11 18:1 reduces cancer risk in rats. J. Nutr. 133:2893-2900.

Demaree, S. R., C. D. Gilbert, H. J. Mersmann and S. B. Smith. 2002. Conjugated linoleic acid differentially modifies fatty acid composition in subcellular fractions of muscle and adipose tissue but not adiposity of postweanling pigs. J. Nutr. 132: 3272-3279.

Drouin, G., A. Douillette, P. Lacasse and B. Paquette. 2004. Effet radiosensibilisateur de l'acide linoleique conjugue chez les cellules cancereuses du sein MCF-7 et MDA-MB-231. Can. J. Physiol. Pharmacol. (in French) 2:94-102.

Dunshea, F. R., E. Ostrowska, B. Luxford, R. J. Smits, R. G. Campbell, D. N. D’Souza and B. P. Mullan. 2002. Dietary conjugated linoleic acid can decrease backfat in pigs housed under commercial conditions. Asian-Aust. J. Anim. Sci. 15:1011-1017.

Ealey, K. N., A. El-Sohemy and M. C. Archer. 2002. Effects of dietary conjugated linoleic acid on the expression of uncoupling proteins in mice and rats. Lipids. 37:853-861.

Evans, M., C. Geigerman, J. Cook, L. Curtis, B. Kuebler and M. McIntosh. 2000. Conjugated linoleic acid suppresses triglyceride accumulation and induces apoptosis in 3T3-L1 preadipocytes. Lipids. 35:899-910. crossref(new window)

Evans, M., J. Brown and M. McIntosh. 2002. Isomer-specific effects of conjugated linoleic acid (CLA) on adiposity and lipid metabolism. J. Nutr. Biochem. 13:508.

Falvella, F. S., R. M. Pascale, M. Gariboldi, G. Manenti, M. R. De Miglio, M. M. Simile, T. A. Dragani and F. Feo. 2002. Stearoyl-CoA desaturase 1 (Scd1) gene overexpression is associated with genetic predisposition to hepatocarcinogenesis in mice and rats. Carcinogenesis. 23:1933-1936.

Gillis, M. H., S. K. Duckett, J. S. sackman and D. H. Keisler. 2003. Effect of rumen-protected conjugated linoleic acid (CLA) or linoleic acid on leptin and CLA content of bovine adipose depots. J. Anim. Sci. 81 (Suppl.2):12 (Abstr.).

Granlund, L., L. K. Juvet, J. I. Pederson and H. I. Nebb. 2003. Trans10, cis12-conjugated linoleic acid prevents triacylglycerol accumulation in adipocytes by acting as a PPAR$\gamma$ modulator. J. Lipid Res. 44:1441-1452.

Griinari, J.M. and D.E. Bauman. 1999. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In: Advances in Conjugated Linoleic Acid Research. Vol. 2. (Ed. J. Sebedio, W. W. Christie and R. Adolf). AOCS Press, Champaign, IL, pp. 180-200.

Ha, Y. L., N. K. Grimm and M. W. Pariza. 1987. Anticarcinogens from fried ground beef: Heat-altered derivatives of linoleic acid. Carcinogenesis. 8:1881-1887.

Henriksen, E. J., M. K. Teachey, Z. C. Taylor, S. Jacob, A. Ptock, K. Kramer and O. Hasselwander. 2003. Isomer-specific actions of conjugated linoleic acid on muscle glucose transport in the obese Zucker rat. Am. J. Physiol Endocrinol. Metab. 285:E98-E105.

Hubbard, N. E., D. Lim and K. L. Erickson. 2003. Effect of separate conjugated linoleic acid isomers on murine mammary tumorigenesis. Cancer Lett. 190:13-19.

Hubbard, N. E., D. Lim, L. Summers and K. L. Erickson. 2000. Reduction of murine mammary tumor metastasis by conjugated linoleic acid. Cancer Lett. 150:93-100.

Ip, C., S. F. Chin, J. A. Scimeca and M. W. Pariza. 1991. Mammary cancer prevention by conjugated dienoic derivative of linoleic acid. Cancer res. 51:6118-6124.

Ip, C., Y. Dong, M. M. Ip, S. Banni, G. Carta, E. Angioni, E. Murru, S. Spada, M. P. Melis and A. Saebo. 2002. Conjugated linoleic acid isomers and mammary cancer prevention. Nutr. Cancer. 43:52-58.

Ip, C., S. Banni, E. Angioni, G. Carta, J. MacGinley, H. J. Thompson, D. Barbano and D. Bauman. 1999. Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. J. Nutr. 129:2135-2142.

Ip, C., J. A. Scimeca and H. Thompson. 1995. Effect of timing and duration of dietary conjugated linoleic acid on mammary cancer prevention. Nutr. Cancer 26:149-157.

Ip, M. M., P. A. Massai-Welch and C. Ip. 2003. Prevention of mammary cancer with conjugated linoleic acid: role of the stroma and the epithelium. J. Mammary Gland Biol. Neoplasia. 8:103-118.

Iwakiri, Y., D. A. Sampson and K. G. Allen. 2002. Suppression of cyclooxygenase -2 and inducible nitric oxide synthase expression by conjugated linoleic acid in murine macrophages. Prostaglandins Leukot. Essent. Fatty Acids. 67:435-443.

Jensen, R. G. 2002. The composition of bovine milk lipid: January 1995 to December 2000. J. Dairy Sci. 85:295-350.

Kang, K. and M. W. Pariza. 2001. Trans-10, cis-12-conjugated linoleic acid reduces leptin secretion from 3T3-L1 adipocytes. Biochem. Biophys. Res. Commun. 287:377-382.

Kelley, D. S., P. C. Taylor, I. L. Rudolph, P. Benito, G. J. Nelson, B. E. Mackey and K. L. Erickson. 2000. Dietary conjugated linoleic acid did not alter immune status in young healthy women. Lipids. 35:1065-1071.

Kemp, M. Q., B. D. Jeffy and D. F. 2003. Conjugated linoleic acid inhibits cell proliferation through a p53-dependent mechanism: Effects on the expression of G1-restriction points in breast and colon cancer cells. J. Nutr. 133:3670-3677.

Kepler, C. R., W. P. Tucker and S. B. Tove. 1966. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. J. Biol. Chem. 241:1350-1354.

Khanal, R. C. and K. C. Olson. 2004. Factors affecting conjugated linoleic acid (CLA) content in milk, meat, and egg: A review. Pakistan J. Nutr. (In press).

Kim, E. J., J. G. Jun, H. S. Park, S. M. Kim, Y. L. Ha and J. H. Park. 2002a. Conjugated linoleic acid (CLA) inhibits growth of Caco-2 colon cancer cells: possible mediation by oleamide. Anticancer Res. 22:2193-2197.

Kim, M. R., Y. Park, K. J. Albright and M. W. Pariza. 2002b. Differential responses of hamsters and rats fed high-fat or lowfat diets supplemented with conjugated linoleic acid. Nutr. Res. 22:715-722.

Kim, Y. J., K. W. Lee and H. J. Lee. 2004. Total antioxidant capacity of arginine-conjugated linoleic acid (CLA) complex. J. Agric. Food Chem. 52:439-444.

Knekt, P. and R. Jarvinen. 1999. Intake of dairy products and breast cancer risk. In (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, M. W. Pariza and G. J. Nelson). Advances in Conjugated Linoleic Acid Research. Vol. I. AOCS Press, Champaign, IL, pp. 444-468.

Kritchevsky, D. 2000. Antimutagenic and some other effects of conjugated linoleic acid. Br. J. Nutr. 83:459-465.

Kritchevsky, D. 2003. Conjugated linoleic acid in experimental atherosclerosis. In: Advances in Conjugated Linoleic Acid Research. Vol. 2. (J. Sebedio, W. W. Christie and R. Adolf). AOCS Press, Champaign, IL, pp. 292-301.

Kritchevsky, D., S. A. Tepper, S. Wright, P. Tso and S. K. Czarnecki. 2000. Influence of conjugated linoleic acid (CLA) on establishment and progression of atherosclerosis in rabbits. J. Am. College Nutr. 19:472S-477S.

Lavillonniere, F., V. Chajes, J. C. Martin, J. L. Sebedio, C. Lhuillery and P. Bougnoux. 2003. Dietary purified cis-9, trans-11 conjugated linoleic acid isomer has anticarcinogenic properties in chemically induced mammary tumors in rats. Nutr. Cancer. 45:190-194.

Lavillonniere, F. and P. Bougnoux. 1999. Conjugated linoleic acid (CLA) and the risk of breast cancer. In: (Ed. M. P. Yurawecz, M. M. Mossoba, J. K. G. Kramer, M. W. Pariza and G. J. Nelson). Advances in Conjugated Linoleic Acid Research. Vol. I. AOCS Press, Champaign, IL, pp. 276-282.

Lee, K. N., D. Kritchevsky and M. W. Pariza. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis. 108:19-25.

Leung, Y. H. and R. H. Liu. 2000. Trans-10, cis-12- conjugated linoleic acid isomer exhibits stronger oxyradical scavenging capacity than cis-9, trans-11-conjugated linoleic acid isomer. J. Agric. Food Chem. 48:5469-5475.

Li, Y. and B. A. Watkins. 1998. Conjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acids. Lipid. 33:417-425.

Liu, J., B. Chen, R. Liu and G. Lu. 1999. Inhibitory effect of conjugated linoleic acid on human gastric carcinoma cell line. Wei Sheng Yan Jiu (In Chinese). 28:353-355.

Liu, J. R., B. Q. Chen, Y. M. Yang, X. L. Wang, Y. B. Xue, Y. M. Zheng and R. H. Liu. 2002. Effect of apoptosis on gastric adenocarcinoma cell line SGC-7901 induced by cis-9, trans- 11-conjugated linoleic acid. World J. Gastroenterol. 8:999-1004.

Loor, J. J., X. Lin and J. H. Herbein. 2003. Effects of dietary cis 9, trans 11-18:2, trans 10 cis 12-18:2 or vaccenic acid (trans 11-18:1) during lactation on body composition, tissue fatty acid profiles, and litter growth in mice. Br. J. Nutr. 90:1039-1048.

Lumeij, J. T. 1997. Avian Clinical Biochemistry. In: Clinical Biochemistry of Domestic Animals $5^{th}$ Edition. (Ed. J. J. Kaneko, J. W. Harvey and M. L. Bruss). Academic Press pp. 857-883.

Majumdar, B., K. W. J. Wahle, S. Moir, A. Schofield, S. N. Choe, A. Farquharson, I. Grant and S. D. Heys. 2002. Conjugated linoleic acids (CLAs) regulate the expression of key apoptotic genes in human breast cancer cells. FASEB J.10.1096/fj.01-0720fje.

Masso-Welch, P. A., D. Zangani, C. Ip, M. M. Vaughan, S. Shoemaker, R. A. Ramirez and M. M. Ip. 2002. Inhibition of angiogenesis by the cancer chemopreventive agent conjugated linoleic acid. Cancer Res. 62:4383-4389. J. Nutr. 134:299-307.

McDonald, H. B. 2000. Conjugated linoleic acid and disease prevention: a review of current knowledge. J. Am. College Nutr. 19:111S-118S.

Medina, E. A., W. F. Horn, N. L. Keim, P. J. Hvel, P. Benito, D. S. Kelley, G. J. Nelson and K. L. Erickson. 2000. Conjugated linoleic acid supplementation in humans: effects on circulating leptin concentrations and appetite. Lipids 35:783-788.

Miller, A., C. Stanton and R. Devery. 2002. Cis 9, trans 11- and trans 10, cis 12-conjugated linoleic acid isomers induce apoptosis in cultured SW480 cells. Anticancer Res. 22:3879-3887.

Miller, A., E. McGrath, C. Stanton and R. Devery. 2003a. Vaccenic acid (t11-18:1) is converted to c-9, t-11 CLA in MCF-7 and SW480 cancer cells. Lipids 38:623-632.

Miller, A., C. Stanton, J. Murphy and R. Devery. 2003b. Conjugated linoleic acid (CLA)-enriched milk fat inhibits growth and modulates CLA-responsive biomarkers in MCF-7 and SW480 human cancer cell lines. Br. J. Nutr. 90:877-885.

Miner, J. L., C. A. Cederberg, M. K. Nielsen, X. Chen and C. A. Baile. 2001. Conjugated linoleic acid (CLA), body fat, and apoptosis. Obes. Res. 9:129-134.

Moon, E. J., Y. M. Lee and K. W. Kim. 2003. Anti-angiogenic activity of conjugated linoleic acid on basic fibroblast growth factor-induced angiogenesis. Oncol. Rep. 10:617-621.

Mougios, V., A. Matsakas, A. Petridou, S. Ring, A. Sagredos, A. Melissopoulou, N. Tsigilis and M. Nicholaidis. 2001. Effect of supplementation with conjugated linoleic acid on human serum lipids and body fat. J. Nutr. Biochem. 12:585-594.

Moya-Camarena, S. Y., J. P. Vanden Huevel, S. G. Blanchard, L. A. Leesnitzer and M. A. Belury. 1999. Conjugated linoleic acid is a potent naturally occurring ligand and activator of PPAR$\alpha$. J. Lipid Res. 40:1426-1433.

Nichenametla, S., E. South and J. Exon. 2004. Interaction of conjugated linoleic acid, sphingomyelin, and butyrate on formation of colonic aberrant crypt foci and immune functions in rats. J. Toxicol. Environ. Health A. 67:469-481.

Nicolosi, R. J., E. J. Rogers, D. Kritchevsky, J. A. Scimeca and P. J. Huth. 1997. Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery. 22:266-277.

Noone, E. J., H. M. Roche, A. P. Nugent and M. J. Gibney. 2002. The effect of dietary supplementation using isomeric blends of conjugated linoleic acid on lipid metabolism in healthy human subjects. Br. J. Nutr. 88:243-251.

Ntambi, J. M., M. Miyazaki, J. Stoehr, H. Lan, C. M. Kendziorski, B. S. Yandell, Y. Song, P. Cohen, J. M. Friedman and A. Attie. 2002. Loss of stearoyl-CoA desaturatse-1 function protects mice against adiposity. PNAS. 99:11482:11486.

Ochoa, J. J., A. J. Farquharson, I. Grant, L. E. Moffat, S. D. Heys and K. W. J. Wahle. 2004. Conjugated linoleic acids (CLA’s) decrease prostate cancer cell proliferation: different molecular mechanisms for cis-9, trans-11 and trans-10, cis-12 isomers. Carcinogenesis (advance access).