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Genetic Relationships of Cattle Breeds Assessed by PCR-RFLP of the Bovine Mitochondrial DNA D-loop Region
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 Title & Authors
Genetic Relationships of Cattle Breeds Assessed by PCR-RFLP of the Bovine Mitochondrial DNA D-loop Region
Yoon, Du Hak; Lee, Hak Kyo; Oh, Sung Jung; Hong, Ki Chang; Jeon, Gwang Joo; Kong, Hong Sik; Lee, Jun Heon;
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To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Taq I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.
Bos Taurus;Bos Indicus;Mitochondrial DNA;PCR-RFLP;Genetic Distance;
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Amano, T., Y. Miyakoshi, T. Takada, Y. Kikkawa and H. Suzuki. 1994. Genetic variants of ribosomal DNA and mitochondrial DNA between swamp and river buffaloes. Anim. Genet. 25:29-36.

Anderson, S., A. T. Bankier, B. G. Barrell, M. H. L. de Bruijn, A. R. Coulson, J. Drouin, I. C. Eperon, D. P. Nierlich, B. A. Roe, F. Sanger, P. H. Schreier, A. J. H. Smith, R. Staden and I. G. Young. 1981. Sequence and organization of the human mitochondrial genome. Nature 290:457-465.

Anderson, S., M. H. L. de Bruljn and A. R. Coulson. 1982. Complete Sequence of Bovine Mitochondrial DNA. Conserved Features of the Mammalian Mitochondrial Genome. J. Mol. Biol. 156:683-717.

Attardy, G. 1985. Animal mitochondrial DNA: An extreme example of genetic economy. Int. Rev. Cytol. 93:93-145.

Bhat, P. P., B. P. Mishra and P. N. Bhat. 1990. Polymorphism of Mitochondrial DNA (mtDNA) in Cattle and Buffaloes. Biochemical Genetics 28:311-318.

Bibb, M. J., R. A. van Etten, C. T. Wright, M. W. Walberg and D. A. Clayton. 1981. Sequence and Gene Organization of Mouse Mitochondrial DNA. Cell 26:167-180.

Boettcher, P. J., A. E. Freeman and S. D. Johnston. 1996. Relationships between Polymorphism for mitochondrial Deoxyribonucleic Acid and Yield Traits of Holstein Cows. J. Dairy Sci. 79:647-654.

Bradley, D. G., D. E. MacHugh, P. Cunningham and R. T. Loftus. 1996. Mitochondrial diversity and the origins of African and European cattle. Proc. Natl. Acad. Sci. USA. 93:5131-5135.

Brown, W. M., M. George and A. C. Wilson. 1979. Rapid evolution of animal mitochondrial DNA. Proc. Natl. Acad. Sci. USA. 76:1967-1971.

Brown, D. R., C. M. Koehler, G. L. Lindberg, A. E. Freeman, J. E. Mayfield, A. M. Myers, M. M. Schutz and D. C. Beitz. 1989. Molecular analysis of cytoplasmic genetic variation in holstein cows. J. Anim. Sci. 67:1926-1932.

Cao. Y., J. Adachi, A. Janke, S. Paabo and M. Hasegawa. 1994. Phylogenetic relationships among eutherian orders estimated from inferred sequences of mitochondrial proteins: instability of tree based on a single gene. J. Mol. Evol. 39:519-527.

Eledath, F. M. and H. C. Hines. 1996. Detection of nucleotide variations in the D-loop region of bovine mitochondrial DNA using polymerase chain reaction-based methodologies. Anim. Genet. 27:333-336.

Felius, M. 1995. Cattle Breeds- An Encyclopedia. Misset. Doetinchem. The Netherlands.

Gadaleta, G., G. Pete, G. de Candia, C. Quagliariello, E. Sibisa and C. Saccone. 1989. The complete nucleotide sequence of the Rattus norvegicus mitochondrial geneome: Cryptic signals revealed by comparative analysis between vertebrates. J. Mol. Evol. 28:497-516.

Giles, R. E., H. Blanc, H. M. Cann and D. C. Wallace. 1980. Maternal inheritance of human mitochondrial DNA. Proc. Natl. Acad. Sci. USA. 77:6715-6719.

Hiendleder, S., H. Lewalski, R. Wassmuth and A. Janke. 1998. The Complete Mitochondrial DNA Sequence of the Domestic Sheep (Ovis aries) and Comparison with the OtherMajor Ovine Haplotype. J. Mol. Evol. 47:441-448.

Hutchison III, C. A., J. E. Newbold, S. S. Potter and M. H. Edgell. 1974. Maternal inheritance of mammalian mitochondrial DNA. Nature 251:536-538.

Kikkawa, Y., T. Amano and H. Suzuki. 1995. Analysis of Genetic Diversity of Domestic Cattle in East and Southeast Asia in Terms of Variations in Restriction Sites and Sequences of Mitochondrial DNA. Biochemical Genetics 33:51-60.

Kim, K. S., S. E. Lee, H. W. Jeong and J. H. Ha. 1998. The complete Nucleotide Sequence of the Domestic Dog (Canis familiaris) Mitochondrial Genome. Mol. Phyl. Evol. 10:210-220.

Kocher, T. D., W. K. Thomas, A. Meyer, S. V. Edwards, S. Paabo, F. X. Villablanca and A. C. Wilson. 1989. Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. USA 86:6196-6200.

Loftus, R. T., D. E. MacHugh, L. O. Ngere, D. S. Balain, A. M. Badi, D. G. Bradley and E. P. Cunningham. 1994a. Mitochondrial genetic variation in European, African and Indian cattle populations. Anim. Genet. 25:265-271.

Loftus, R.T., D. E. MacHugh and D. G. Bradley, P. M. Sharp and P. Cunningham. 1994b. Evidence for two independent domestications of cattle. Proc. Natl. Acad. Sci. USA. 91:2757-2761.

Mannen, H., T. Kojima and K. Oyama. 1998a. Effect of Mitochondrial DNA Variation on Carcass Traits of Japanese Black Cattle. J. Anim. Sci. 76:36-41.

Mannen, H., S. Tsuji, R. T. Loftus and D. G. Bradley. 1998b. Mitochondrial DNA Variation and Evolution of Japanese Black Cattle (Bos taurus). Genetics 150:1169-1175.

Mignotte, F., M. Gueride, A. M. Champagne and J. C. Mounolou. 1990. Direct repeats in the non-coding region of rabbit mitochondrial DNA: Involvement in the generation of intraand inter-individual heterogeneity. Eur. J. Biochem. 194:561- 571.

Miller, S. A., D. D. Dykes and H. F. Polesky. 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16:1215.

Nei, M. 1972. Genetic distance between populations. Am. Nat. 106:283-292.

Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583-590.

Nei, M., F. Tajima and Y. Tateno. 1983. Accuracy of estimated phylogenetic trees from molecular data. J. Mol. Evol. 19:153-170.

Pegoraro, L., Z. Yang, S. Samaké, F. V. Meirelles, V. Bordingnon, L. Moquin and L. C. Smith. 1996. Sequence comparison of mitochondrial tRNA genes and origin of light strand replication in Bos taurus and Mellore (Bos indicus) breeds. Anim. Genet. 27:91-94.

Pesole, G. and E. Sbisa. 1992. The evolution of the mitochondrial D-loop region and the origin of modern man. Mol. Biol. Evol. 9:587-598.

Ron, M., O. Yoffe and J. I. Weller. 1993. Sequence variation in Dloop mtDNA of cow lineages selected for high and low maternal effects on milk production. Anim. Genet. 24:183-186.

Saccone, C., G. Pesole and E. Sbisa. 1991. The main regulatory region of mammalian mitochondrial DNA: Structure-function model and evolutionary pattern. J. Mol. Evol. 33:83-91.

Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.

Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. 2nd Edition. Cold Spring Harbor Laboratory Press.

Sudhir Kumar, Koichiro Tamura, Ingrid B. Jakobsen and Masatoshi Nei. 2001. MEGA2: Molecular Evolutionary Genetics Analysis software, Bioinformatics 17(12):1244-1245.

Takeda, K. and A. Onishi. 1995. SSCP analysis of pig mitochondrial DNA D-loop region polymorphism. Anim. Genet. 26:321-326.

Troy, C. S., D. E. MacHugh, J. F. Bailey, D. A. Magee, R. T. Loftus, P. Cunningham, A. T. Chamberlain, B. C. Sykes and D. G. Bradley. 2001. Genetic evidence for Near-Eastern origins of European cattle. Nature 410:1088-1091.

Watanabe, T., Y. Hayashi, R. Semba and N. Ogasawara. 1985. Bovine Mitochondrial DNA Polymorphism in Restriction Endonuclease Cleavage Patterns and the Location of the Polymorphic Sites. Biochemical Genetics 23:947-957.

Watanabe, T., J. S. Masangkay, S. Wakana, N. Saitou and T. Tomita. 1989. Mitochondrial DNA Polymorphism in Native Philippine Cattle Based on Restriction Endonuclease Cleavage Patterns. Biochemical Genetics 27:431-438.

Wilkinson, G. S. and A. M. Chapman. 1991. Length and sequence variation in evening bat D-loop mtDNA. Genetics 128:607-617.

Xu, X. and U. Arnason. 1994. The complete mitonchondrial DNA sequence of the horse, Equus caballus: Extensive heteroplasmy of the control region. Gene 148:357-362.

Yeh, F. C., R. C. Yang, T. Boyle, Z. H. Ye and J. X. Mao. 1997. POPGENE, the User-finedly Shareware for Population Genetic Analysis Molecular and Biotechnology Centre University of Alberta, Alberta.