Advanced SearchSearch Tips
New Evidences of Effect of Melanocortin-4 Receptor and Insulin-like Growth Factor 2 Genes on Fat Deposition and Carcass Traits in Different Pig Populations
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
New Evidences of Effect of Melanocortin-4 Receptor and Insulin-like Growth Factor 2 Genes on Fat Deposition and Carcass Traits in Different Pig Populations
Chen, J.F.; Xiong, Y.Z.; Zuo, B.; Zheng, R.; Li, F.E.; Lei, M.G.; Li, J.L.; Deng, C.Y.; Jiang, S.W.;
  PDF(new window)
The Melanocortin-4 Receptor (MC4R) and Insulin-like Growth Factor 2 (IGF2) are two important candidate genes related to fat deposition and carcass traits. MC4R was found on study on human obesity and then was studied as candidate gene affecting food intake and fat deposition traits in mice and pigs. Insulin-like Growth Factor 2 (IGF2) gene plays an important role on tumor cell proliferation and muscle growth. It also affects fat traits and live weight in pigs. In this paper, MC4R and IGF2 were studied as two candidate genes associated with important economic traits such as fat deposition and carcass traits in five different pig populations. Taq I-PCR-RFLP and Bcn I-PCR-RFLP were respectively used to detect the polymorphism of genotypes of MC4R and IGF2 genes. Different MC4R genotype frequencies were observed in four populations. IGF2 genotype frequencies were also different in two populations. The results of association analysis show both MC4R and IGF2 genes were significantly associated with fat deposition and carcass traits in about 300 pigs. This work will add new evidence of MC4R and IGF2 affecting fat deposition and carcass traits in pigs and show that two genes can be used as important candidate genes for marker assistant selection (MAS) of growth and lean meat percentage in pigs.
MC4R Gene;IGF2 Gene;Fat Deposition and Carcass Traits;Different Pig Populations;
 Cited by
Postnatal Expression Pattern of Adipose Type Fatty Acid Binding Protein in Different Adipose Tissues of Porcine,;;;;;

아세아태평양축산학회지, 2007. vol.20. 6, pp.811-816 crossref(new window)
New Evidence of Alleles (V199I and G52S) at the PRKAG3 (RN) Locus Affecting Pork Meat Quality,;;;;;;;;;;;;;

아세아태평양축산학회지, 2008. vol.21. 4, pp.471-477 crossref(new window)
The Asp298Asn missense mutation in the porcine melanocortin-4 receptor (MC4R) gene can be used to affect growth and carcass traits without an effect on meat quality, animal, 2007, 1, 08  crossref(new windwow)
Challis, B. G., L. E. Pritchard, J. W. Creemers, J. Delplanque, J. M. Keogh, J. Luan, N. J. Wareham, G. S. Yeo, S. Bhattacharyya, P. Froguel, A. White, I. S Farooqi and S. O'Rahilly. 2002. A missense mutation disrupting a dibasic prohormone processing site in pro-opiomelanocortin (POMC) increases susceptibility to early-onset obesity through a novel molecular mechanism. Hum. Molec. Genet. 11:1997-2004.

Ciobanu, D., J. Bastiaansen, M. Malek, J. Helm, J. Woollard, G. Plastow and M. Rothschild. 2001. Evidence for new alleles in the protein kinase adenosine monophosphate-activated $r_3$-subunit gene associated with low glycogen content in pig skeletal muscle and improved meat quality. Genetics 159:1151-1162.

Fan, W., B. A. Boston, R. A. Kesterson, V. J. Hruby and R. D. Cone. 1997. Role of melanocortinergic neurons in feeding and the agouti obesity syndrome, Nature 385:165-168.

Florini, J. R., K. A. Magri, D. Z. Ewton, P. L. James, K. Grindstaff and P. S. Rotwei. 1991. Spontaneous differentiation of skeletal myoblast is dependent upon autocrine secretion of insulin-like growth factor-II. J. Biol. Chem. 266:15917-15923.

Gantz, I., H. Miwa, Y. Konda, Y. Shimoto, T. Tashiro, S. J. Watson, J. Delvalle and T. Yamada. 1993. Molecular cloning, expression, and gene localization of a fourth melanocortin receptor. J. Biol. Chem. 68:15174-15179.

Huszar, D., C. A. Lynch, V. Fairchild-Huntress, J. H. Dunmore, Q. Fang, L. R. Berkemeier, W. Gu, R. A. Kesterson, B. A. Boston, R. D. Cone, F. J. Smith, L. A. Campfield, P. Burn and F. Lee. 1997. Targeted disruption of the melanocortin-4 receptor results on obesity in mice. Cell 88:131-141.

Ho, G. and R. G. MacKenzie. 1999. Functional characterization of mutations in melanocortin-4 receptor associated with human obesity. J. Biol. Chem. 274:35816-31822.

Jeon, J. T., O. Carlborg, A. Tornsten, E. Giuffra, V. Amarger, P. Chardon, L. Andersson-Eklind, K. Andersson, I. Hansson, K. Lundstrom and L. Andersson. 1999. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pigs maps to the IGF2 locus. Nature Genetics 21:157-158. crossref(new window)

Jiang, S. W., J. Peng and Y. Z. Xiong. 2002. Melanocortin receptor involvement in the regulation of food intake and energy homeostasis. Hereditas (Beijing) 24(2):223-226.

Jones, J. I. and D. R. Clemmons. 1995. Insulin-like growth factors and their binding proteins: biological actions. Endocr. Rev. 16:3-34.

Kim, J. Y., D. H. Yoon, B. L. Park, L. H. Kim, K. J. Na, J. G. Choi, C. Y. Cho, H. K. Lee, E. R. Chung, B. C. Sang, I. J. Cheong, S. J. Oh and H. D. Shin. 2005. Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene. Asian-Aust. J. Anim. Sci. 18(1):3-7.

Kim, K. S., N. J. Larsen and M. F. Rothschild. 2000. Linkage and physical mapping of the porcine melanocortin-4 receptor (MC4R) gene. J. Anim. Sci. 78:791-792.

Kim, K. S., N. J. Larsen, T. Short, G. Plastow and M. F. Rothschild. 2000b. A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mamm Genome 11:131-135.

Knoll, A., L. Putnova, J. Dvorak and S. Cepica. 2000. A NciI PCRRFLP within intron 2 of the porcine insulin-like growth factor 2 (IGF2) gene. Anim. Genet. 31:150-151.

Lamberson, W. R., J. A. Sterle and R. L. Matteri. 1996. Relationships of serum insulin-like growth factor-II concentrations to growth, compositional,and reproductive traits of swine. J. Anim. Sci. 74:1753-1756.

Latham, K. E., A. S. Doherty, C. D. Scott and R. M. Schultz. 1994. Igf2r and Igf2 gene expression in androgenetic, gynogenetic, and parthenogenetic preimplantation mouse embryos: absence of regulation by genomic imprinting. Genes Dev. 8(3):290-9.

Liu, G. L., S. W. Jiang, Y. Z. Xiong, R. Zheng, J. Peng and Y. C. Qu. 2002. Molecular screening of MC4R gene and association with fat traits in pig resource family. Acta Genetica Sinica. 29(6):497-501.

Liu, G. L., S. W. Jiang, Y. Z. Xiong, R. Zheng, J. Peng and Y. C. Qu. 2003. Association of PCR-RFLP polymorphisms of IGF2 gene with fat deposition related traits in pig resouse family. Acta Genetica Sinica. 30(12):1107-1112.

MacNeil, D. J., A. D. Howard, X. Guan, T. M. Fong, R. P. Nargund, M. A. Bednarek, M. T. Goulet, D. H. Weinberg, A. M. Strack, D. J. Marsh, H. Y. Chen, C. P. Shen, A. S. Chen, C. I. Rosenblum, T. MacNeil, M. Tota, E. D. MacIntyre and L. H. Van der Ploeg. 2002. The role of melanocortins in body weight regulation: opportunities for the treatment of obesity. Eur J. Pharmacol. 450:93-109.

MacIntyre, D. E. and S. B. Glueck. 2003. The proof of the pudding is in the eating: Editorial Focus on 'Hyperphagia, not hypometabolism, causes early onset obesity in melanocortin-4-receptor knockout mice' Physiol. Genomics. 13:11-14.

Yu, H. and T. Rohan. 2000. Role of the Insulin-Like Growth Factor Family in Cancer Development and Progression. Journal of the National Cancer Institute 92(18):1472-1489.

Yeo, G. S., I. S. Farooqi, S. Aminian, D. J. Halsall, R. G. Stanhope and S. O'Rahilly. 1998. A frameshift mutation in MC4R associated with dominantly inherited human obesity. Nature Genetics 20:111-112.

Magri, K. A., M. R. Benetict, D. Z. Ewton and J. R. Florini. 1994. Negative feedback regulation of insulin-like growth factor-II gene expression in difereniation myoblasts. in vitro. Endocrinology 135:53-62.

Nezer, C., L. Moreau, B. Brouwers, W. Coppieters, J. Detilleux, R. Hanset, L. Karim, A. Kvasz, P. Leroy and M. Georges. 1999. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pigs. Nature Genetics 21:155-156.

Kolarikova, O., L. Putnova, T. Urban, J. Adamek, A. Knoll and J. Dvorak. 2003. Associations of the IGF2 gene with growth and meat efficiency in Large White pigs. J. Appl. Genet. 4(44): 509-513.

Yerle, M., G. Echard, A. Robic, A. Mairal, C. Dubut-Fontana, J. Riquet, P. Pinton, D. Milan, Y. Lahbib-Mansais and J. Gellin. 1996. A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cell Genet. 73:194-202.