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The Expression Characterization of Chicken Uncoupling Protein Gene
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 Title & Authors
The Expression Characterization of Chicken Uncoupling Protein Gene
Zhao, Jian-Guo; Li, Hui; Wang, Yu-Xiang; Meng, He;
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 Abstract
The UCPs are members of the mitochondrial inner membrane transporter family, present in the mitochondrial inner membrane. Their main function is increasing the energy expenditure via diminishing the resulting production of ATP from mitochondrial oxidative phosphorylation instead of yielding dissipative heat. They are associated with the metabolism of fat and regulation of energy expenditure. The UCP gene can be viewed as the candidate gene for chicken fatness. In the present study, RT-PCR and Northern Blot methods were developed to investigate the expression of the UCP gene in ten tissues including heart, liver, spleen, lung, kidney, gizzard, intestine, brain, breast muscle and abdominal fat of chicken. The results of both RT-PCR and Northern Blot methods showed that the UCP gene expressed specific in breast muscle. The expression levels of UCP gene in breast muscles from egg-type and meat-type chickens of hatching, 2, 4, 6 and 8 wk of age were detected by RT-PCR assay and results showed that the expression levels of UCP gene were related to breeds. Expression level of UCP gene in layers was higher than that in broilers at various weeks of age except at 6 wk. The UCP gene's expression was higher at 6 wk and had no significant difference among other weeks of age in broilers; in layers the expression level of UCP gene had no significant difference among weeks of age. The experiment results also showed that insulin could increase the expression level of UCP gene by 40% compared with control group.
 Keywords
Chicken;UCP Gene;RT-PCR;Northern Blot;Insulin;
 Language
English
 Cited by
1.
Association between SNP Marker of Uncoupling Protein 3 Gene and Meat Yield and Marbling Score Traits in Korean Cattle,;;;

한국축산식품학회지, 2011. vol.31. 4, pp.530-536 crossref(new window)
 References
1.
Evock-Clover, C. M., S. M. Poch, M. P. Richards, C. M. Ashwell and J. P. McMurtry. 2002. Expression of an uncoupling protein gene homolog in chickens. Comparative Biochemistry and Physiology, Part A 133:345-358.

2.
Fleury, C., M. Neverova, S. Collins, S. Raimbault, O. Champigny, C. Levi-Meyrueis, F. Bouillaud, M. F. Seldin, R. S. Surwit, D. Ricquier and C. H. Warden. 1997. Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nature Genetics 15:269-272.

3.
Friedman, J. M. 2000. Obesity in the new millennium. Nature 404:632-634.

4.
Gura, T. 1998. Uncoupling proteins provide new clue to obesity's Causes. Science 280(29):1369-1370. crossref(new window)

5.
Ikeobi, C. O. N., J. A. Woolliams, D. R. Morrice, A. Law, D. Windsor, D. W. Burt and P. M. Hocking. 2002. Quantitative trait loci affecting fatness in the chicken. Anim. Genet. (33):428-435. crossref(new window)

6.
Jin, J. H., T. M. Zhang, E. Y. Zhang and Y. Li. 2000. Regulatory effecs of fasting and insulin on expression of uncoupling protein-1, 2, 3 genes. China J. Biochem. Mol. Biol. 16(3):394-399.

7.
Kim, J. H., and M. G. Baik. 2004. Production of leptin in E. coli and its effect on glucose and acetate transport tissues of Korean cattle (Hanwoo). Asian-Aust. J. Anim. Sci. 17(8):1062-1068.

8.
Meng, H., H. Li, J. G. Zhao and Z. L. Gu. 2004. Differential expression of peroxisome proliferator- activated receptors alpha and gamma gene in various chicken tissues. Domestic Anim. Endocrinol. 28(1):105-110.

9.
Meng, H., J. G. Zhao, Z. H. Li and H. Li. 2005. Single nucletide polymorphism on Peroxisome Proliferator-Activated Receptor genes associated with fatness traits in chicken. Asian-Aust. J. Anim. Sci. In press.

10.
Raimbault, S., S. Dridi, F. Denjean, J. Lachuer, E. Couplan, F. Bouillaud, A. Bordas, C. Duchamp, M. Taouis and D. Ricquier. 2001. An Uncoupling protein homologue putatively involved in facultative muscle thermogenesis in birds. Biocheim. J. 353:441-444. crossref(new window)

11.
Ravussin, E., S. Lillioja, W. C. Knowler, L. Christin, D. Freymond, W. G. Abbott, V. Boyce, B. V. Howard and C. Bogardus. 1988. Reduced rate of energy as a risk factor for body-weight gain. New Eng. J. Med. 318:467-472.

12.
Ricquier, D. and F. Bouillaud. 2000. The Uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP. Biochem. J. 345:161-179.

13.
Roberts, S. B., J. Savage, W. A. Coward, B. Chew and A. Lucas. 1988. Energy expenditure and intake in infants born to lean and overweight mothers. New Eng. J. Med. 318:461-466.

14.
Rothschild, M. F. and M. Soller. 1997. Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe. 8:13-20.

15.
Shen, T. and J. Y. Wang. 1990. Biochemistry, Higher Education Press.

16.
Surwit, R. S., S. Y. Wang, A. E. Petro, D. Sanchis, S. Raimbault, D. Ricquier and S. Collins. 1998. Diet-induced changes in uncoupling proteins in obesity-prone and obesity-resistant strains of mice. Proc. Natl. Acad. Sci. 95:4061-4065.

17.
Wang, H., D. X. Sun, Y. Yu, D. Wang and Y. Zhang. 2005. Relationship between differential gene expreesion in ovary and heterosis of egg number traits in a chicken Diallel Cross. Asian-Aust. J. Anim. Sci. 18(6):767-771.

18.
Wang, Y. F., M. Yu, B. Liu, B. Fan, H. Wang, M. J. Zhu and K. Li. 2004. Full length cDNA, genomic organizations and expression profiles of the porcine proteasomal ATPases PSMC5 gene. Asian-Aust. J. Anim. Sci. 17(7):897-902.

19.
Zhao, J. G., H. Li, H. Meng, Z. L. Gu, Q. G. Wang and Y. X. Wang. 2002. The study on the uncoupling protein gene as the candidate gene for fatness traits in chicken. Acta Genetica Sinica. 29(6):481-486.

20.
Zhou, Y. T., M. Shimabukuro, K. Koyama, Y. Lee, M. Y. Wang, F. Trieu, C. B. Newgard and R. H. Unger. 1997. Induction by leptin of uncoupling protein-2 and enzyme of fatty acid oxidation. Proc. Natl. Acad. Sci. 94:6386-6390.