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The Relationships between Plasma Insulin-like Growth Factor (IGF)-1 and IGF-Binding Proteins (IGFBPs) to Growth Pattern, and Characteristics of Plasma IGFBPs in Steers
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The Relationships between Plasma Insulin-like Growth Factor (IGF)-1 and IGF-Binding Proteins (IGFBPs) to Growth Pattern, and Characteristics of Plasma IGFBPs in Steers
Lee, H.G.; Hidari, H.; Kang, S.K.; Hong, Z.S.; Xu, C.X.; Kim, S.H.; Seo, K.S.; Yoon, D.H.; Choi, Y.J.;
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This study was conducted to determine the characteristics of IGFBPs in plasma of steers, and to profile the relationship between growth and plasma IGF-1 and IGFBPs with aging in Holstein steers. Four blots of IGFBP at molecular weights of 38-43, 34, 29-32 and 24 kDa bands were detected by western ligand blot assay using . On the basis of immunoblotting with anti-bovine IGFBP-2 and -3 antiserums, we observed the band for IGFBP-2 at approximately 34 kDa, and the IGFBP-3 band was detected at 38-43 kDa and 34 kDa in adult steers and calves. The IGFBP-3 antiserum used on the blots exhibited significant cross-reactivity with 34 kDa IGFBP-2. Furthermore, the 38-43 kDa IGFBP-3 bands were reduced to a 36 kDa band after deglycosylation, whereas the 34 kDa IGFBP-2 was intact. The plasma IGF-1, IGFBP-3 and other IGFBPs showed stability throughout a whole day. The change in live weight was found to be positively correlated to the plasma IGF-1 concentration (r = 0.6801, n = 64, p<0.05) and plasma IGFBP-3 (r = 0.6321, n = 64, p<0.05), while inversely correlated to plasma IGFBP-2 (r = -0.2919, n = 64, p<0.05). Furthermore, plasma IGF-1 was positively correlated to plasma IGFBP-3 (r = 0.6191, p<0.001), but was not correlated to plasma IGFBP-2. The portion of IGFBP-2 for total IGFBPs in calves was higher than in adult steers (p<0.05) and was decreased with growth, whereas that of IGFBP-3 was increased with increased live weight (p<0.05). The ratio IGFBP-3 for IGFBP-2 (BP-3/BP-2) was increased with growing of liveweight. Therefore, the changes in plasma IGF-1 level with increased liveweight may be related to the changes in plasma IGFBP-3 level and IGFBP-2 may give an important role in anabolic action of IGF-1 with the growth of body during calfhood in Holstein steers.
IGF-1;IGFBPs;Growth Stages;Steer;
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Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 9, pp.1334-1341 crossref(new window)
Argente, J., V. Barrios, J. Pozo, M. T. Munoz, F. Hervas and M. Stene. 1993. Hernandez. Normative data for insulin-like growth factors (IGFs), IGF binding proteins, and growth hormone binding protein in a healthy Spanish pediatric population: age- and sex-related changes. J. Clin. Endocrinol. Metab. 77:1522-1528.

Bar, R. S., M. Boes, B. L. Dake, A. Sandra, M. L. Bayne, M. A. Cascieri and B. A. Booth. 1990. Tissue localization of perfused endothelial cell IGF binding protein is markedly altered by association with IGF-1. Endocrinology 127:3243-3245.

Bark, T. H., M. A. McNurlan, C. H. Lang and P. J. Garlick. 1998. Increased protein synthesis after acute IGF-I or insulin infusion is localized to muscle in mice. Am. J. Physiol. Endocrinol. Metab. 275:E118-E123.

Baxter, R. C. 1991. Insulin-like growth factor (IGF) binding proteins the role of serum IGFBPs in regulating IGF availability. Acta. Paediatr. Scand (Supply), 372:107-114.

Baxter, R. C. and J. L. Martin. 1986. Radioimmunoassay of growth hormone-dependent insulin-like growth factor binding protein in human plasma. J. Clin. Invest. 78:1504-1512.

Baxter, R. C., J. L. Martin and V. A. Beniac. 1989. High molecular weight insulin-like growth factor binding protein complex. Purification and properties of the acid-labile subunit from human serum. J. Biol. Chem. 15:264(20):11843-11848.

Bishop, M. D., R. C. M. Simmen, F. A. Simmen and M. E. Davis. 1991. The relationship of insulin-like growth factor-I with post-weaning performance in Angus beef cattle. J. Anim. Sci. 67:2872-2880.

Blum, W. F. and M. B. Ranke. 1990. Insulin-like growth factor binding proteins (IGFBPs) with special reference to IGFBP-3. Acta. Paediatr. Scand. 367:55-62.

Cohick, W. S. and D. R. Clemmons. 1991. Regulation of insulinlike growth factor binding protein synthesis and secretion in a bovine epithelial cell line. Endocrinology 129:1347-1354.

Davis, T. A., M. L. Fiorotto, D. G. Burrin, R. C. Vann, P. J. Reeds, H. V. Nguyen, P. R. Beckett and J. A. Bush. 2002. Acute IGF-I infusion stimulates protein synthesis in skeletal muscle and other tissues of neonatal pigs. Am. J. Physiol. Endocrinol. Metab. 283:E638-E647.

Daughaday, W. H., I. K. Mariz and S. L. Blethen. 1980. Inhibition of assess of bound somatomedin to membrane receptor and immunobinding sites: A comparison of radioreceptor and radioimmunoassay of somatomedin in native and acid-ethanolextracted serum. J. Clin. Endocrinol. Metab. 51:781-788.

Davenport, M. L., D. R. Clemmons, M. V. Miles, C. Camacho-Hubner, A. J. D'Ercole and L. E. Underwood. 1990. Regulation of serum insulin-like growth factor-I (IGF-I) and IGF binding proteins during rat pregnancy. Endocrinology 127(3):1278-1286.

Funston, R. N., G. E. Moss and A. J. Roberts. 1995. Insulin-like growth factor-1 (IGF-1) and IGF-binding protein in bovine sera and pituitaries at different stages of the estrous cycle. Endocrinology 136:62-68.

Funston, R. N., G. E. Seidel Jr, J. Klindt and A. J. Roberts. 1996. Insulin-like growth factor 1 and insulin-like growth factorbinding proteins in bovine serum and follicular fluid before and after the preovulatory surge of luteinizing hormone. Biol. Reprod. 55:1390-1396.

Gomez, J. M., F. J. Maravall, N. Gomez, M. A. Navarro, R. Casamitjana and J. Soler. 2003. Interactions between serum leptin, the insulin-like growth factor-I system, and sex, age, anthropometric and body composition variables in a healthy population randomly selected. Clin. Endocrinol. 58(2):213-219.

Guler, H. P., J. Zapf, C. Schmid and E. R. Froesch. 1989. Insulinlike growth factors I and II in healthy man. Estimations of half-lives and production rates. Acta. Endocrinol. (Copenh), 121:753-758.

Hossenlopp, P., D. Seurin, B. Segovia-Quinson, S. Hardouin and M. Binoux. 1986. Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding proteins and competitive binding studies. Anal. Biochem. 154:138-143.

Hossner, K. L., R. H. McCusker and M. V. Dodson. 1997 Insulinlike growth factors and their binding proteins in domestic animals. Br. Soc. Anim. Sci. 64:1-15.

Juul, A., P. Dalgaard, W. F. Blum, P. Bang, K. Hall, K. F. Michaelsen, J. Muller and N. E. Skakkebaek. 1995. Serum levels of insuli-like growth factor (IGF)-binding protein-3 (IGFBP-3) in healthy infants, children, and adolescents: the relation to IGF-I, IGF-II, IGFBP-1, IGFBP-2, age, sex, body mass index, and pubertal maturation. J. Clin. Endocrinol. Metab. 76:1610-1616.

Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteiophage T4. Nature (London), 227:680-685.

Lee, C. Y., F. W. Bazer, T. D. Etherton and F. A. Simmen. 1991. Ontogeny of insulin-like growth factors (IGF-I and IGF-II) and IGF-binding proteins in porcine serum during fetal and postnatal development. Endocrinol. 128:2336-2344.

Lee, H. G., R. A. Vega, L. T. Phung, N. Matsunaga, H. Kuwayama, and H. Hidari. 2000. The effect of growth hormone releasing peptide-2 (KP102) administration on plasma insulin-like growth factor (IGF)-I and IGF-binding proteins in Holstein steers on different planes of nutrition. Dome Anim. Endocrinol. 18(3):293-308.

NRC. 1998. Nutrient Requirements of Beef Cattle. National Research Council, Academy Press.

Rajaram, S., D. J. Baylink and S. Mohan. 1997. Insulin-like growth factor-binding proteins in serum and other biological fluids: regulation and functions. Endocrine Reviews, 18(6):801-831.

Roberts, C. A., S. N. McCutcheon, H. T. Blair, P. D. Gluckman and B. H. Breier. 1990. Developmental patterns of plasma insulin-like growth factor-1 concentrations in sheep. Domestic Anim. Endocrinol. 7:457-464.

Ronge, H. and J. Blum. 1989. Insulin-like growth factor I during growth in bulls. Reprod. Nutr. Dev. 29(1):105-111.

Schwander, J. and J. L. Mary. 1993. The RIA for IGFBP-2 in mana meager catch? The RIA for IGFBP-2 in man-a meager catch? Growth Regul. 3:104-108.

Vega, R. A., H. G. Lee, N. Matsunaga, H. Kuwayama and H. Hidari. 2002. Age-related changes in plasma leptin from early growing to late finishing stages of castrated Holstein steers: utilizing multi-species leptin RIA. Asian-Aust. J. Anim. Sci. 15(5):725-731.

Vega, R. A., H. Hidari, H. Kuwayama, M. Suzuki and D. D. Manalo. 2004. The relationships of plasma leptin, backfat thickness and TDN intake across finishing stage of Holstein steers. Asian-Aust. J. Anim. Sci. 17(3):330-336.

Wood, W. I., G. Cachianes, W. J. Henzel, G. A. Winslow, S. A. Spenser, R. Hellmiss, J. L. Martin and R. C. Baxter. 1988. Cloning and expression of the growth hormone-dependent insulin-like growth factor-binding protein. Mol. Endocrinol. 2:1176-1185.

Yang, Y. W. H., J. F. Wang, C. C. Orlowski, S. P. Nissley and M. M. Rechler. 1989. Structure, Specificity, and regulation of the insulin-like growth factor-binding proteins in adult rat serum. Endocrinol. 125:1540-1555.

Zapf, J. 1995. Physiological role of the insulin-like growth factor binding proteins. Europ. J. Endocrinol. 132:645-654.