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Effects of Testosterone, 17β-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes
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Effects of Testosterone, 17β-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes
Oh, Young Sook; Cho, Sang Bum; Baek, Kyung Hoon; Choi, Chang Bon;
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The aim of this study was to investigate the effects of testosterone, 17-estradiol, and progesterone on the differentiation of bovine intramuscular adipocytes (BIA). Stromal-vascular (SV) cells were obtained from M. longissimus dorsi of 20 months old Korean (Hanwoo) steers, and were cultured in DMEM containing 5% FBS. The proliferated BIA were induced to differentiate with 0.25 M dexamethasone, 0.5 mM 1-methyl-3-isobutyl-xanthine and 10 g/ml insulin. During differentiation, the cells were treated with testosterone, 17-estradiol, and progesterone at concentrations of , , and M, respectively, for 12 days. Regardless of its concentration, testosterone remarkably reduced lipid droplets in the cytosol of BIA. On the other hand, 17-estradiol and progesterone increased the accumulation of lipid droplets in BIA. Testosterone significantly (p<0.05) decreased GPDH activities with a dose-dependent pattern. 17-Estradiol treatment onto BIA during differentiation, however, increased GPDH activity showing the highest activity (11.3 nmol/mg protein/min) at M. Treatment of BIA with progesterone also increased (p<0.05) GPDH activity with the highest activity (13.8 nmol/mg protein/min) at M. In conclusion, the results in the current study suggest that testosterone inhibits differentiation of BIA by suppressing GPDH activity while 17-estradiol and progesterone have adverse effects.
Testosterone;17-estradiol;Progesterone;Bovine Adipocyte;GPDH;
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Ailhaud, G., P. Grimaldi and R. Negrel. 1994. Hormonal regulation of adipose dfferentiation. Trends Endocrinol. 5:132-136.

Bailey, C. M., C. L. Probert and V. R. Bohman. 1966. Growth rate, feed utilization and body composition of young bulls and steers. J. Anim. Sci. 25:132-137.

Cornelius, P., O. A. MacDougald and M. D. Lane. 1994. Regulation of adipocyte development. Annu. Rev. Nutr. 14:99-129.

Dieudonne, M. N., R. Pecquery, M. C. Leneveu and Y. Giudicelli. 2000. Opposite effects of androgens and estrogens on adipogenesis in rat preadipocytes: Evidence for sex and siterelated specificities and possible involvement of insulin-like growth factor 1 receptor and peroxisome proliferator-activated receptor $\gamma$2. Endocrinol. 141:649-656.

Gregory, K. E., S. C. Seideman and J. J. Ford. 1983. Effects of late castration, zeranol and breed group on composition and palatability characteristics of longissimus muscle of bovine males. J. Anim. Sci. 56:781-786.

Hamosh, M. and P. Hamosh. 1975. The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue. J. Clin. Invest. 55:1132-1135.

Kim, H. J. and R. K. Kalkhoff. 1975. Sex steroid influence on triglyceride metabolism. J. Clin. Invest. 56:888-896.

Kissebah, A. H. and G. R. Krakower. 1994. Regional adiposity and morbidity. Physiol. Rev. 74:761-811.

Lacasa, D., X. Le Liepvre, P. Ferre and I. Dugail. 2001. Progesterone stimulates adipocyte determination and differentiation 1/sterol regulatory element-binding protein 1c gene expression. Potential mechanism for the lipogenic effect of progesterone in adipose tissue. J. Biol. Chem. 276:11512-11516.

Monjo, M., A. M. Rodriguez, A. Palou and P. Roca. 2003. Direct effects of testosterone, 17beta-estradiol, and progesterone on adrenergic regulation in cultured brown adipocytes: potential mechanism for gender-dependent thermogenesis. Endocrinol. 144:4923-4930.

Nishizawa, H., I. Shimomura, K. Kishida, N. Maeda, H. Kuriyama, H. Nagaretani, M. Matsuda, H. Kondo, N. Furuyama, S. Kihara, T. Nakamura, Y. Tochino, T. Funahashi and Y. Matsuzawa. 2002. Androgens decrease plasma adiponectin, an insulin-sensitizing adipocyte-derived protein. Diabetes. 51:2734-2741.

Palin, S. L., P. G. McTernan, L. A. Anderson, D. W. Sturdee, A. H. Barnett and S. Kumar. 2003. 17$\beta$-estradiol and anti-estrogen ICI: compound 182,780 regulate expression of lipoprotein lipase and hormone-sensitive lipase in isolated subcutaneous abdominal adipocytes. Metabolism 52:383-388.

Prins, J. B. and S. O’Rahilly. 1997. Regulation of adipose cell number in man. Clin. Sci. 92:3-11.

Rebuffe-Scrive, M. 1987. Sex steroid hormones and adipose tissue metabolism in variectomized and adrenalectomized rats. Acta Physiol. Scand. 129:471-477.

Rodriguez, A. M., M. Monjo, P. Roca and A. Palou. 2002. Opposite actions of testosterone and progesterone on UCP1 mRNA expression in cultured brown adipocytes. Cell. Mol. Life Sci. 59:1714-1723.

Roncari, D. A. K. and R. L. R. Van. 1978. Promotion of human adipocyte precursor replication by 17$\beta$-estradiol in culture. J. Clin. Invest. 62:503-508.

Seong, H. H., K. S. M. Min, H. Kang, J. T. Yoon, H. J. Jin, H. J. Chung, W. K. Chang, S. G. Yun and Kunio Shiota. 2003. Changes in Ovarian and Placental 20$\alpha$-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat. Asian-Aust. J. Anim. Sci. 16:342-347.

Singh, R., J. N. Artaza, W. E. Taylor, N. F. Gonzalez-Cadavid and S. Bhasin. 2003. Androgens stimulate myogenic differentiation and inhibit adipogenesis in C3H 10T1/2 pluripotent cells through an androgen receptor-mediated pathway. Endocrinol. 144:5081-5088.

Smas, C. M. and H. S. Sul. 1995. Control of adipocyte differentiation. Biochem. J. 309:697-710.

Tomita, T., T. Yonekura, T. Okada and E. Hayashi. 1984. Enhancement in cholesterol-esterase activity and lipolysis due to 17$\beta$-estradiol treatment in rat adipose tissue. Horm. Metabolism Res. 16:525-528.

Valette, A., J. M. Meignen, L. Mercier, J. G. Liehr and J. Boyer. 1986. Effects of 2-fluoroestradiol on lipid metabolism in the ovariectomized rat. J. Steroid Biochem. 25:575-578.

Xu, X., G. De Pergola, P. S. Eriksson, L. Fu, B. Carlsson, S. Yang, S. Eden and P. Bjorntorp. 1993. Postreceptor events involved in the up-regulation of beta-adrenergic receptor mediated lipolysis by testosterone in rat white adipocytes. Endocrinol. 132:1651-1657.