JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effects of Testosterone, 17β-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Testosterone, 17β-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes
Oh, Young Sook; Cho, Sang Bum; Baek, Kyung Hoon; Choi, Chang Bon;
  PDF(new window)
 Abstract
The aim of this study was to investigate the effects of testosterone, 17-estradiol, and progesterone on the differentiation of bovine intramuscular adipocytes (BIA). Stromal-vascular (SV) cells were obtained from M. longissimus dorsi of 20 months old Korean (Hanwoo) steers, and were cultured in DMEM containing 5% FBS. The proliferated BIA were induced to differentiate with 0.25 M dexamethasone, 0.5 mM 1-methyl-3-isobutyl-xanthine and 10 g/ml insulin. During differentiation, the cells were treated with testosterone, 17-estradiol, and progesterone at concentrations of , , and M, respectively, for 12 days. Regardless of its concentration, testosterone remarkably reduced lipid droplets in the cytosol of BIA. On the other hand, 17-estradiol and progesterone increased the accumulation of lipid droplets in BIA. Testosterone significantly (p<0.05) decreased GPDH activities with a dose-dependent pattern. 17-Estradiol treatment onto BIA during differentiation, however, increased GPDH activity showing the highest activity (11.3 nmol/mg protein/min) at M. Treatment of BIA with progesterone also increased (p<0.05) GPDH activity with the highest activity (13.8 nmol/mg protein/min) at M. In conclusion, the results in the current study suggest that testosterone inhibits differentiation of BIA by suppressing GPDH activity while 17-estradiol and progesterone have adverse effects.
 Keywords
Testosterone;17-estradiol;Progesterone;Bovine Adipocyte;GPDH;
 Language
English
 Cited by
1.
Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells,;;;;;;;;

아세아태평양축산학회지, 2007. vol.20. 4, pp.501-510 crossref(new window)
2.
Testicular Expression of Steroidogenic Enzyme Genes Is Related to a Transient Increase in Serum 19-nortestosterone during Neonatal Development in Pigs,;;;;;;;;;;

아세아태평양축산학회지, 2007. vol.20. 12, pp.1832-1842 crossref(new window)
1.
Differentially expressed proteins associated with myogenesis and adipogenesis in skeletal muscle and adipose tissue between bulls and steers, Molecular Biology Reports, 2012, 39, 2, 953  crossref(new windwow)
2.
Comparison between Single and Co-culture of Adipocyte and Muscle Cell Lines in Cell Morphology and Cytosolic Substances, Journal of Animal Science and Technology, 2012, 54, 2, 103  crossref(new windwow)
 References
1.
Ailhaud, G., P. Grimaldi and R. Negrel. 1994. Hormonal regulation of adipose dfferentiation. Trends Endocrinol. 5:132-136.

2.
Bailey, C. M., C. L. Probert and V. R. Bohman. 1966. Growth rate, feed utilization and body composition of young bulls and steers. J. Anim. Sci. 25:132-137.

3.
Cornelius, P., O. A. MacDougald and M. D. Lane. 1994. Regulation of adipocyte development. Annu. Rev. Nutr. 14:99-129.

4.
Dieudonne, M. N., R. Pecquery, M. C. Leneveu and Y. Giudicelli. 2000. Opposite effects of androgens and estrogens on adipogenesis in rat preadipocytes: Evidence for sex and siterelated specificities and possible involvement of insulin-like growth factor 1 receptor and peroxisome proliferator-activated receptor $\gamma$2. Endocrinol. 141:649-656.

5.
Gregory, K. E., S. C. Seideman and J. J. Ford. 1983. Effects of late castration, zeranol and breed group on composition and palatability characteristics of longissimus muscle of bovine males. J. Anim. Sci. 56:781-786.

6.
Hamosh, M. and P. Hamosh. 1975. The effect of estrogen on the lipoprotein lipase activity of rat adipose tissue. J. Clin. Invest. 55:1132-1135.

7.
Kim, H. J. and R. K. Kalkhoff. 1975. Sex steroid influence on triglyceride metabolism. J. Clin. Invest. 56:888-896.

8.
Kissebah, A. H. and G. R. Krakower. 1994. Regional adiposity and morbidity. Physiol. Rev. 74:761-811.

9.
Lacasa, D., X. Le Liepvre, P. Ferre and I. Dugail. 2001. Progesterone stimulates adipocyte determination and differentiation 1/sterol regulatory element-binding protein 1c gene expression. Potential mechanism for the lipogenic effect of progesterone in adipose tissue. J. Biol. Chem. 276:11512-11516.

10.
Monjo, M., A. M. Rodriguez, A. Palou and P. Roca. 2003. Direct effects of testosterone, 17beta-estradiol, and progesterone on adrenergic regulation in cultured brown adipocytes: potential mechanism for gender-dependent thermogenesis. Endocrinol. 144:4923-4930.

11.
Nishizawa, H., I. Shimomura, K. Kishida, N. Maeda, H. Kuriyama, H. Nagaretani, M. Matsuda, H. Kondo, N. Furuyama, S. Kihara, T. Nakamura, Y. Tochino, T. Funahashi and Y. Matsuzawa. 2002. Androgens decrease plasma adiponectin, an insulin-sensitizing adipocyte-derived protein. Diabetes. 51:2734-2741.

12.
Palin, S. L., P. G. McTernan, L. A. Anderson, D. W. Sturdee, A. H. Barnett and S. Kumar. 2003. 17$\beta$-estradiol and anti-estrogen ICI: compound 182,780 regulate expression of lipoprotein lipase and hormone-sensitive lipase in isolated subcutaneous abdominal adipocytes. Metabolism 52:383-388.

13.
Prins, J. B. and S. O’Rahilly. 1997. Regulation of adipose cell number in man. Clin. Sci. 92:3-11.

14.
Rebuffe-Scrive, M. 1987. Sex steroid hormones and adipose tissue metabolism in variectomized and adrenalectomized rats. Acta Physiol. Scand. 129:471-477.

15.
Rodriguez, A. M., M. Monjo, P. Roca and A. Palou. 2002. Opposite actions of testosterone and progesterone on UCP1 mRNA expression in cultured brown adipocytes. Cell. Mol. Life Sci. 59:1714-1723.

16.
Roncari, D. A. K. and R. L. R. Van. 1978. Promotion of human adipocyte precursor replication by 17$\beta$-estradiol in culture. J. Clin. Invest. 62:503-508.

17.
Seong, H. H., K. S. M. Min, H. Kang, J. T. Yoon, H. J. Jin, H. J. Chung, W. K. Chang, S. G. Yun and Kunio Shiota. 2003. Changes in Ovarian and Placental 20$\alpha$-hydroxysteroid Dehydrogenase Activity during the Pregnancy in the Rat. Asian-Aust. J. Anim. Sci. 16:342-347.

18.
Singh, R., J. N. Artaza, W. E. Taylor, N. F. Gonzalez-Cadavid and S. Bhasin. 2003. Androgens stimulate myogenic differentiation and inhibit adipogenesis in C3H 10T1/2 pluripotent cells through an androgen receptor-mediated pathway. Endocrinol. 144:5081-5088.

19.
Smas, C. M. and H. S. Sul. 1995. Control of adipocyte differentiation. Biochem. J. 309:697-710.

20.
Tomita, T., T. Yonekura, T. Okada and E. Hayashi. 1984. Enhancement in cholesterol-esterase activity and lipolysis due to 17$\beta$-estradiol treatment in rat adipose tissue. Horm. Metabolism Res. 16:525-528.

21.
Valette, A., J. M. Meignen, L. Mercier, J. G. Liehr and J. Boyer. 1986. Effects of 2-fluoroestradiol on lipid metabolism in the ovariectomized rat. J. Steroid Biochem. 25:575-578.

22.
Xu, X., G. De Pergola, P. S. Eriksson, L. Fu, B. Carlsson, S. Yang, S. Eden and P. Bjorntorp. 1993. Postreceptor events involved in the up-regulation of beta-adrenergic receptor mediated lipolysis by testosterone in rat white adipocytes. Endocrinol. 132:1651-1657.