Advanced SearchSearch Tips
Effect of Non-starch Polysaccharides and Resistant Starch on Mucin Secretion and Endogenous Amino Acid Losses in Pigs
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Non-starch Polysaccharides and Resistant Starch on Mucin Secretion and Endogenous Amino Acid Losses in Pigs
Morel, Patrick C.H.; Melai, J.; Eady, S.L.; Coles, G.D.;
  PDF(new window)
Generally, dietary fibre (DF) includes lignin, non-starch polysaccharides (NSP) and resistant starch (RS). In monogastric species, low levels of dietary fibre in the diet are associated with various diseases and high levels reduce nutrient digestibilities. In this study, the effects of different types and levels of NSP (soluble: -glucan, insoluble cellulose) and resistant starch on mucin secretion and endogenous nitrogen and amino acid losses in pigs were investigated. A total of 25 five-week-old weaner pigs (9.5 kg1.5 kg), were randomly allocated to each of five experimental diets. Different levels of purified barley -glucan (BG) extract (5 or 10% of -glucan, providing 4 or 8% of BG in the diet), and resistant starch (RS) (8.3 or 16.6% of Hi-, providing 5 or 10% RS in the diet) were substituted for wheat starch in a purified diet in which enzymatically-hydrolysed casein was the sole source of protein. The diets were fed for 21 days. No statistically significant difference between treatments (p>0.05) was observed for growth performance and organs weights. No difference in ileal starch digestibility was observed between pigs on the cellulose or -glucan diets. However, as the level of resistant starch in the diet increased the ileal starch digestibility decreased (p<0.05). The inclusion of resistant starch in the diet (5 or 10%) did not increase mucin production when compared with the cellulose-only diet. However, as the level of beta-glucan in the diet increased, both crude mucin in the digesta dry matter and per kg dry matter intake increased (p<0.05). Pigs fed the diet containing 8% of beta-glucan had higher endogenous loss flow than those fed the diets including 5 or 10% of resistant starch or 4% of -glucan. In conclusion, dietary inclusion of resistant starch increased the level of starch reaching the large intestine without any effect on mucin secretion, or endogenous nitrogen or amino acid losses content in the small intestine. The addition of -glucan to a diet containing cellulose increases both mucin secretion and endogenous amino acid and nitrogen losses in the small intestine.
Non-starch Polysaccharides;Mucin;Endogenous Amino Acid Losses;Pigs;
 Cited by
Gelatinized Carbohydrates in the Diet of Catla catla Fingerlings: Effect of Levels and Sources on Nutrient Utilization, Body Composition and Tissue Enzyme Activities,;;;;;

아세아태평양축산학회지, 2007. vol.20. 1, pp.89-99 crossref(new window)
Impacts of resistant starch and wheat bran consumption on enteric inflammation in relation to colonic bacterial community structures and short-chain fatty acid concentrations in mice, Gut Pathogens, 2016, 8, 1  crossref(new windwow)
Effects of a diet high in resistant starch on fermentation end-products of protein and mucin secretion in the colons of pigs, Starch - Stärke, 2017, 1600032  crossref(new windwow)
New insight into the role of resistant starch in pig nutrition, Animal Feed Science and Technology, 2015, 201, 1  crossref(new windwow)
Increasing dietary oat fibre decreases the permeability of intestinal mucus, Journal of Functional Foods, 2016, 26, 418  crossref(new windwow)
The impact of dietary fibres on the physiological processes governing small intestinal digestive processes, Bioactive Carbohydrates and Dietary Fibre, 2015, 6, 2, 117  crossref(new windwow)
Anderson, J. W. 1986. Fibre and health: an overview. Am. J. Gastroenterol. 81:892-897.

Anugwa, F. O. I., V. H. Varel, J. S. Dickson, W. G. Pond and L. P. Krook. 1989. Effects of dietary and protein concentration on growth, feed efficiency, visceral organ weights and large intestine microbial population in swine. J. Nutr. 119:879-886.

Brouns, F., B. Kettlitz and E. Arrigoni. 2002. Resistant starch and the 'butyrate revolution'. Trends Food Sci. Tech. 13:251-261.

Butts, C. A., P. J. Moughan, W. C. Smith and D. H. Carr. 1993a. Endogenous lysine and other amino acid flows at the terminal ileum of the growing pig (20 kg bodyweight): The effect of protein-free, synthetic amino acid, peptide and protein alimentation. J. Sci. Food Agric. 61:31-40.

Butts, C. A., P. J. Moughan, W. C. Smith, G. W. Reynolds and D. J. Garrick. 1993b. The effect of food dry matter intake on endogenous ileal amino acid excretion determined under peptide alimentation in the 50 kg liveweight pig. J. Sci. Food Agric. 62:235-243.

de Lange, C. F. M., W. C. Sauer, R. Mosenthin and W. B. Souffrant. 1989. The effects of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and faeces of pigs. J. Anim. Sci. 67:746-754.

Englyst, H. N., M. E. Quigley and G. J. Hudson. 1994. Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic, high-performance chromatographic or spectrophotometric measurement of constituent sugars. Analyst. 119:1497-1509.

Englyst, H. N., S. M. Kingsman, G. J. Hudson and J. H. Cummings. 1996. Measurement of resistant starch in vitro and in vivo. Br. J. Nutr. 75:749-755.

Fernandez, J. A. and J. N. Jorgensen. 1986. Digestibilities and absorption of nutrients as affected by fibre content in the diet of the pig. Quantitative aspects. Livest. Prod. Sci. 15:53-71.

Furuya, S. and Y. Kaji. 1992. The effects of feed intake and purified cellulose on the endogenous ileal amino acid flow in growing pigs. Br. J. Nutr. 68:463-472.

Graham, H., K. Hesselman and P. Amman. 1986. Influence of wheat bran and sugar- beet pulp of the digestibility of dietary components in a cereal-based pig diet. J. Nutr. 116:242-251.

Granger, D. 1997. Kjeldahl versus Dumas-which gives the right answer? Chem. New Zealand, 7.

Hodgkinson, S. M., P. J. Moughan, G. W. Reynolds and K. A. C. James. 2000. The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig. Br. J. Nutr. 83:412-430.

Jacobs, L. R. 1986. Dietary fiber and gastrointestinal cell proliferation. In: (Ed. G. V. Vahouny and D. Kritchevsky), Dietary Fiber: Basic and Clinical Aspects. Plenum Press, New York. pp. 211-228.

Jorgensen, H., H. Q. Zhao and B. Eggum. 1996. The influence of dietary and environmental temperature on the development of the gastro-intestinal tract, digestibility, degree of fermentation in the hind-gut and energy metabolism in pigs. Br. J. Nutr. 75:365-378.

Leterme, P., E. Froidmont, F. Rossi and A.Thewis. 2000. Effect of barley fibres and barley intake on the ileal endogenous nitrogen losses in piglets. J. Cereal Sci. 31:229-239.

Leterme, P., E. Froidmont, F. Rossi and A.Thewis. 1998. The high water-holding capacity of pea inner fibres affects the ileal flow of endogenous amino acids in pigs. J. Agric. Food Chem. 46:1927-1934.

Leterme, P., A. Thewis, P. van Leeuwen, T. Monmart and J. Huisman. 1996. Chemical composition of pea fibre isolate and their effect on endogenous amino acid flow at the ileum of the pig. J. Sci. Food Agric. 72:127-134.

Leterme, P., L. Pirard and A. Thewis. 1992. A note on the effect of wood cellulose level in protein-free diets on the recovery and endogenous protein collected from ileum of pigs. Anim. Prod. 54:163-165.

Lien, K. A., M. I. McBurney, B. I. Beyde, A. B. R. Thomson and W. C. Sauer. 1996. Ileal recovery of nutrients and mucin in humans fed total enteral formulas supplemented with soy fiber. Am. J. Clin. Nutr. 63(4):584-595.

Lien, K. A., W. C. Sauer and J. M. He. 2001. Dietary influence on the secretion into and degradation of mucin in the digestive tract of monogastric animals and humans. J. Anim. Feed. Sci. 10(2):223-245.

Lien, K. A., W. C. Sauer and M. Fenton. 1997. Mucin output of ileal digesta of pigs fed a protein-free diet. Z. Ernaehrungswiss. 36:182-190.

Mariscal-Landin, G., B. Seve, Y. Colleaux and Y. Lebreton. 1995. Endogenous amino nitrogen collected from pigs with end-toend ileorectal anastomosis is affected by the method of estimation and altered by dietary fiber. J. Nutr. 125:136-146.

Martinez-Puig, D., J. F. Perez, M. Catillo, A. Andaluz, M. Anguita, J. Morales and J. Gasa. 2003. Consumption of raw potato starch increases colon lenght and feacal excretion of purine bases in growing pigs. J. Nutr. 133:134-139.

Montagne, L., J. R. Pluske and D. J. Hampson. 2003. A review of interaction between dietary fibre and intestinal mucosa, and their consequences on digestive health in young non-ruminat animals. Anim. Feed Sci. Tech. 108:95-117.

Morel, P. C. H., R. M. Padilla and G. Ravindran. 2003. Effect of Non-Starch Polysaccharides on Intestinal Mucin Production and Amino Acid Endogenous Losses in Pigs. Asian-Aust. J. Anim. Sci. 16(9):1332-1338.

Morel, P. C. H., R. M. Padilla, Y. H. Cottam and G. D. Coles. 2001. Influence of soluble and insoluble dietary fibre on growth, organ weights and blood metabolites in weaner pigs. Recent Advances in Animal Nutrition in Australia 13:22A.

Morita, T., H. Tanabe, K. Takahashi and K. Sugiyama. 2004. Ingestion of resistant starch protects endotoxin influx from the intestinal tract and reduces D-galactosamine-induced liver injury in rats. Journal of Gastroenterology and Hepatology 19:303-313.

Moughan, P. J., G. Schuttert and M. Leenaars. 1992. Endogenous amino acid flow in stomach and small intestine of the young growing pigs. J. Sci. Food Agric. 60:437-442.

Nutrient Requirements of Swine. 1998. 10th Ed. National Academic Press: Washington, DC.

Pluske, J. R., D. W. Pethick and B. P. Mullan. 1998. Differential effects of feeding fermentable carbohydrate to growing pigs on performance, gut size, and slaughter characteristics. Anim. Sci. 67:147-156.

SAS Institute. 2002. SAS for Window, version 8.02, SAS Institute. Cary, NC.

Sauer, W. C. and L. Ozimec. 1986. Digestibility of amino acids in swine: results and their practical application. A review. Livest. Prod. Sci. 15:367-388.

Short, F. J, P. Gorton, J. Wiseman and K. N. Boorman. 1996. Determination of titanium dioxide added as an inert marker in chicken digestibility studies. Anim. Feed Sci. Tech. 59:215-221.

Souffrant, W. B. 2001. Effect of dietary fibre on ileal digestibility and endogenous nitrogen losses in pig. Anim. Feed. Sci. Tech. 90:93-102.

Trowell, H., D. A. Southgate, T. M. Wolever, A. R. Leeds, M. A. Gassull and D. J. Jenkins. 1976. Letter: dietary fibre refined. Lancet 1:967.

Vahouny, G. V. and M. M. Cassidy. 1986. Dietary fibre and intestinal adaptation. In: Dietary Fiber: Basic and Clinical Aspects. (Ed. G. V. Vahouny and D. Kritchevsky), Plenum Press, New York. pp. 181-209.

Wang, J. F., B. B. Jensen, H. Jorgensen, D. F. Li and J. E. Lindberg. 2002. Anim. Feed Sci. Tech. 102:125-136.

Yin, Y. L. 1994. Nutrients value of feedstuff and diets for pigs: I. Apparent post-ileal digestibility and interrelationships between dietary constituents and faecal and ileal digestibility. Anim. Feed Sci. Tech. 45:243-255.

Zebrowska, T. and J. Kowalczyk. 2000. Endogenous nitrogen losses in monogastrics and ruminants as affected by nutritional factors. Asian-Aust. J. Anim. Sci. 13:210-218.