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Identification of Bovine Lymphocyte Antigen DRB3.2 Alleles in Iranian Golpayegani Cattle by DNA Test
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 Title & Authors
Identification of Bovine Lymphocyte Antigen DRB3.2 Alleles in Iranian Golpayegani Cattle by DNA Test
Mosafer, J.; Nassiry, M.R.;
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The bovine lymphocyte antigen (BoLA)-DRB3 gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. Since different alleles favour the binding of different peptides, DRB3 has been extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. For that reason, the genetic diversity of the bovine class II DRB3 locus was investigated by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). This study describes genetic variability in the BoLA-DRB3 in Iranian Golpayegani Cattle. Iranian Golpayegani Cows (n = 50) were genotyped for bovine lymphocyte antigen (BoLA)-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism method. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, HaeIII and BstYI was conducted on the DNA from Iranian Golpayegani Cattle. In the Iranian Golpayegani herd studied, we identified 19 alleles.DRB3.216 had the highest allelic frequency (14%), followed by DRB3.27 (11%). Six alleles (DRB3.225, 24, 22, 20, 15, 3) had frequencies = 2%. Although additional studies are required to confirm the present findings, our results indicate that exon 2 of the BoLA-DRB3 gene is highly polymorphic in Iranian Golpayegani Cattle.
BoLA-DRB3;Bovine Lymphocyte Antigen;PCR-RFLP;Iranian Golpayegani Cattle;Polymorphism;
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Boom, R., C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E. Wertheim-Van Dillen and J. Van Der Moordea. 1989. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28(3):495-503.

Dietz, A. B., N. D. Cohen, L. Timms and M. E. Kehril, Jr. 1977a. Bovine lymphocyte Antigen class II alleles as risk factors for high somatic cell counts in milk of lactating dairy cows. J. Dairy Sci. 80:406-412.

Dietz, A. B., J. C. Detilleux, A. E. Freeman, D. H. Kelly, J. R. Stabel and M. E. Kehrli, Jr. 1997b. Genetic association of bovine lymphocyte antigen DRB3 alleles with immunological traits of Holstein cattle. J. Dairy Sci. 80:400-405.

Giovambattista, G., C. D. Golijow, F. N. Dulout and M. M. Lojo. 1996. Gene frequencies of DRB3.2 locus of ArgentineCreole cattle. Anim. Genet. 27:55-56.

Gilliespie, B. E., B. M. Jayarao, H. H. Dowlen and S. P. Oliver, 1999. Analysis and frequency of bovine lymphocyte antigen DRB3.2 alleles in Jeresy cows. J. Dairy Sci. 82:2049-2053.

Kelem, S. C., J. C. Deitilleux, A. E. Freeman, M. E. Kehrli, Jr., A. B. Dietz, L. K. Fox, J. E. Butler, I. Kasckovics and D. H. Kelley. 1997. Genetic association between parameters of innate immunity and measures of mastitis in preparturient Holstein cattle. J. Dairy Sci. 80:1767-1775.

Ledwidge, S. A., B. A. mallard, J. P. Gibson, G. B. Jansen and Z. H. Jiang. 2001. Multi-primer target PCR for rapid identification of bovine DRB3 alleles. Anim. Genet. 32:219-221.

Lewin, H. A. 1989. Disease resistance and immune response genes in cattle: strategies for their detection and evidence of their existence. J. Dairy Sci. 72:1334-1348.

Lewin, H. A. 1994. Host genetic mechanism of resistance and susceptibility to a bovine retroviral infection. Anim. Biotechnol. 5:183-191.

Nassiry, M. R., F. Eftekhar shahroodi, J. Mosafer, A. Norouzy and A. Javadmanesh. 2004. Distribution of bovine lymphocyte antigen (BoLA-DRB3) alleles in Iranian Holstein cattle. 11th Asian-Aust. Anim. Prod. Congress (AAAP) in Malaysia. 3:266-268.

Sharif, S., B. A. Mallard, B. N. Wilkie, J. M. Sargeant, H. M Scott, J. C. M. Dekkers and K.E. Leslie. 1998. Associations of the bovine major histocompatibility complex DRB3 (BoLADRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle. Anim. Genet. 29:185-193.

Starkenburg, G. E., I. G. Udina, G. O. Shaikhaev and H. Chester-Jones. 1977. Frequencies and effects of alternative DRB3.2 alleles of bovine lymphocyte antigen for Holstein in milk selection and control lines. J. Dairy Sci. 80:3411.

Sulimova, G. E., I. G. Udina, G. O. Shaikhaev, and I. A. Zakharov. 1995. DNA polymorphism at the BoLA-DRB3 gene of cattle in relation to resistance and Susceptibility to leukemia. Russian J. Genet. 32:1105-1109.

Takeshima, S. N., M. Ikegami, M. Morita, Y. Nakai and Y. Aida. 2001. Identification of new cattle BoLA-DRB3 alleles by sequence-based typing. Immunogenetics. 53:74-81.

Takeshima, S., Y. Nakai, M. Ohta and Y. Aida. 2002. Characterization of DRB3 alleles in the MHC of Japanese Shorthorn Cattle by polymerase chain reaction-sequence-based typing. J. Dairy Sci. 85:1630-1632

Van Eijk, M. J. T., J. A. Stewart-Haynes and H. A. lewin. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Anim. Genet. 23:483-496.