JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Effect of Intraruminal Sucrose Infusion on Volatile Fatty Acid Production and Microbial Protein Synthesis in Sheep
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Intraruminal Sucrose Infusion on Volatile Fatty Acid Production and Microbial Protein Synthesis in Sheep
Kim, K.H.; Lee, S.S.; Kim, K.J.;
  PDF(new window)
 Abstract
Effects of sucrose supplement on the pattern of VFA production and microbial protein synthesis in the rumen were examined in sheep consuming basal diet of grass silage (2.5 kg fresh wt/d) that was provided in 24 equal meals each day by an automatic feeder. Four mature wethers were allocated to four experimental treatments in a 44 Latin square design with periods lasting 14 days. The treatments were (1) the basal diet, (2) supplemented with 150 g sucrose and 7.0 g urea, (3) 300 g sucrose and 13 g urea, and (4) 450 g sucrose and 20 g urea given as a continuous intraruminal infusion for 24 h. All infusions were given in 2 litres of aqueous solution per day using a peristaltic pump. The effect of sucrose level on rumen mean pH was significantly linear (p<0.01). There were not significant differences in the concentration of ammonia-N, total VFA and the molar proportions of acetate, propionate and butyrate with the level of sucrose infusion. The molar proportions of isobutyric acid (p<0.05) and isovaleric acid (p<0.001) were significantly reduced when the infused amount of sucrose was increased. The flow of microbial N was linearly (p<0.001) increased with sucrose and urea level. High levels of readily fermentable carbohydrate in a ration reduced the efficiency of microbial protein synthesis in the rumen. It was demonstrated that of the individual fatty acids, only the molar proportion of isovalerate showed a significant negative correlation (R2=) with the amount of microbial N produced and a significant positive correlation (R2=) with the efficiency of microbial growth.
 Keywords
Branched-chain Fatty Acid;Grass Silage;Microbial Protein Synthesis;Sucrose;
 Language
English
 Cited by
1.
Effects of Synchronization of Energy and Nitrogen Release in the Rumen on the Starch Disappearance in the Gastrointestinal Tract and Growth Performance of Hanwoo Steers,;;;;;;;;;

Journal of Animal Science and Technology, 2011. vol.53. 6, pp.533-540 crossref(new window)
2.
Human Papillomavirus Prevalence and Genotype Distribution among HIV-Infected Women in Korea,;;;;;;;;;

Journal of Korean Medical Science, 2014. vol.29. 1, pp.32-37 crossref(new window)
 References
1.
Allison, M. J. 1969. Biosynthesis of amino acids by ruminal microorganisms. J. Anim. Sci. 28:797-807.

2.
ARC. 1980. The nutrient requirements of ruminant livestock. Agricultural Research Council, Commonwealth Agricultural Bureaus, Farnham Royal, Slough, Berkshire, UK.

3.
ARC. 1984. The nutrient requirements of ruminant livestock. Supplement No 1, Agricultural Research Council, Commonwealth Agricultural Bureaus, Farnham Royal, Slough, Berkshire, UK.

4.
Borchers, R. 1977. Allantoin determination. Anal. Biochem. 79:612-613.

5.
Chamberlain, D. G., P. C. Thomas, W. D. Wilson, C. J. Newbold and J. C. MacDonald. 1985. The effects of carbohydrate supplements on ruminal concentration of ammonia in animals given diets of grass silage. J. Agric. Sci. 104:331-340.

6.
Chamberlain, D. G., P. A. Martin, S. Robertson and E. A. Hunter. 1992. Effects of the type of additive and the type of supplement of the utilization of grass silage for milk production in dairy cows. Grass Forage Sci. 47:391-399.

7.
Cummins, K. A. and A. H. Papas. 1985. Effects of isocarbon-4 and isocarbon-5 volatile fatty acids on microbial protein synthesis and dry matter digestibility in vitro. J. Dairy Sci. 68:2588-2595.

8.
Cushnahan, A., C. S. Mayne and E. F. Unsworth. 1995. Effects of ensilage of grass on performance and nutrient utilization by dairy cattle. 2. Nutrient metabolism and rumen fermentation. Anim. Sci. 60:347-359.

9.
Fujihara, T., E. R Orskov, P. J. Reeds and D. J. Kyle. 1987. The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric infusion. J. Agric. Sci. (Camb.) 109:7-12.

10.
Gunter, S. A., L. J. Krysl, M. B. Judkins, J. T. Broesder and R. K. Barton. 1990. Influence of branched-chain fatty acid supplementation on voluntary intake, site and extent of digestion, ruminal fermentation, digesta kinetics and microbial protein synthesis in beef heifers consuming grass hay. J. Anim. Sci. 68:2885-2892.

11.
Hefner, D. L., L. L. Berger and G. C. Fahey, Jr. 1985. Branchedchain fatty acid supplementation of corn crop residue diets. J. Anim. Sci. 61:1264-1276.

12.
Huhtanen, P. J. 1987. The effects of intraruminal infusion of sucrose and xylose on nitrogen and fiber digestion in the rumen and intestines of cattle receiving diets of grass silage and barley. Journal of Agricultural Science in Filand. 59:405- 424.

13.
Huhtanen, P. J., H. O. Miettinen and V. F. J. Toivonen. 1997. Effects of silage fermentation and post-ruminal casein supplementation in lactating dairy cows. 1. Diet digestion and milk production. J. Sci. Food Agric. 74:450-458.

14.
Judkins, M. B., J. D. Wallace, M. L. Galyean, L. J. Krysl and E. E. Parker. 1987. Passage rates, rumen fermentation and weight change in protein supplemented grazing cattle. J. Range Manage. 40:100-105.

15.
Kim, K. H., Y-G. Oh, J-J. Choung and D. G. Chamberlain. 1999. Effects of varying degree of synchrony of energy and nitrogen release in the rumen on the synthesis of microbial protein in cattle consuming grass silage. J. Sci. Food Agric. 79:833-838.

16.
Kim, K. H., S. S. Lee, B. T. Jeon and C. W. Kang. 2000. Effects of the pattern of energy supply on the efficiency of nitrogen utilization for microbial protein synthesis in the non-lactating cows consuming grass silage. Asian-Aust. J. Anim. Sci. 13 (7):962-966.

17.
Krysl, L. J., M. L. Galyean, M. B. Judkins, M. E. Branine and R. E. Estell. 1987. Digestive physiology of steers grazing fertilized and non-fertilized blue grama rangeland. J. Range Manage. 40:493-501.

18.
Lawes Agricultural Trust. 1990. Genstat 5, Rothamsted Experimental Station, Harpenden, Hertfordshire, UK.

19.
Miettinen, H. O. and P. J. Huhtanen. 1997. Effects of silage fermentation and post-ruminal casein supplementation in lactating dairy cows: 2. Energy metabolism and plama amino acid. J. Sci. Food Agric. 74:459-468.

20.
Oh, Y. G., K. H. Kim, J. J. Choung and D. G. Chamberlain. 1999. The effect of the form of nitrogen in the diet on ruminal fermentation and the yield of microbial protein in sheep consuming diets of grass silage supplemented with starch or sucrose. Anim. Feed Sci. Technol. 78:227-237.

21.
Orskov, E. R. 1982. Protein nutrition in ruminants. Academic press INC. London.

22.
Susmel, P., B. Stefanon, E. Plazzotta, M. Sphanghero and C. F. Mills. 1994. The effect of energy and protein intake on the excretion of purine derivatives. J. Agric Sci. (Camb.) 123:257-265.

23.
Thomas, P. C., D. G. Chamberlain. N. C. Kelly and M. K. Wait. 1980. The nutritive value of silages. Digestion of nitrogenous constituents in sheep receiving diets of grass silage and barley. Br. J. Nutr. 43:469-479.