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Production of Functional Whey Protein Concentrate by Monitoring the Process of Ultrafilteration
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 Title & Authors
Production of Functional Whey Protein Concentrate by Monitoring the Process of Ultrafilteration
Jayaprakasha, H.M.; Yoon, Y.C.;
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This investigation was undertaken in order to elicit the relationship between the extent of ultrafiltration processing of whey and its effect on composition and yield of resultant whey protein concentrate (WPC). Cheddar cheese whey was fractionated through ultrafiltration to an extent of 70, 80, 90, 95, 97.5% and 97.5% volume reduction followed by I stage and II stage diafiltration. After each level of ultrafiltration, the composition of WPC was monitored. Similarly, the initial whey was adjusted to 3.0, 6.2 and 7.0 pH levels and ultrafiltration was carried out to elicit the effect of pH of ultrafiltration on the composition. Further, initial whey was adjusted to different levels of whey protein content ranging from 0.5 to 1.0 per cent and subjected to ultrafiltration to different levels. The various range of retentate obtained were further condensed and spray dried in order to assess the yield of WPC per unit volume of whey used and the quantity of whey required to produce unit weight of product. With the progress of ultrafiltration, there was a progressive increase in protein content and decrease in lactose and ash content. The regression study led to good relationships with values of more than 0.95 between the extents of permeate removed and the resultant changes in composition of each of the constituents. Whey processed at pH 3.0 had significantly a very low ash content and high protein content as compared to processing at 6.2 and 7.0. The yield of WPC per unit volume of whey varied significantly with the initial protein content. Higher initial protein content led to higher yield of all ranges of WPC and the quantity of whey required per unit weight of spray dried WPC significantly reduced. Regression equations establishing the relationship between initial protein content of whey and the yield of various types of WPC have been derived with very high values of 0.99. This study revealed that, the yield and composition of whey can be monitored strictly by controlling the processing parameters and WPC can be produced depending on the food formulation requirement.
Whey;Ultrafiltration;Diafiltration;Processing Parameters;Whey Protein Concentrate;Yield and Composition;
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AOAC. 1980. Handbook, 13th Ed. (Association of Official Analytical Chemists). Washington DC.

Cheryan, M. and K. P. Kuo. 1984. Hollow fibers and spiral wound modules fro ultrafiltration of whey: Energy consumption and performance. J. Dairy Sci. 67:1406-1409.

Daufin, G., F. Michel and U. Merin. 1992. Study of ultrafiltration of defatted WPC withdrawn from an industrial plant. L Lait 72:185-189.

deWit, J. N. 1984. Functional properties of whey proteins in food systems. Neth. Milk Dairy J. 38:71-75.

Derham, O. and S. Chanton. 1986. An empirical mathematical model of retentate composition in ultrafiltration of dairy products. J. Dairy Res. 53:271-274.

Hayes, J. F., J. A. Dunkerly and L. L. Muller. 1974. Studies on whey processing by ultrafiltration. II Improving the permeation rates by preventing fouling. Australian J. Dairy Technol. 29(3):132-136.

Hiddink, J., R. D. E. Boer and R. F. C. Nooy. 1981. Effects of various pretreatments on the ultrafiltration of sweet whey about $55^{\circ}C$. Milchwissenchaft. 36(11):657-662.

Hugunin, A. G. 1987. Application of UF whey protein: Developing a new markets. In Bulletin, Trends in whey utilization. Int. Dairy Fed. Brussels, Belgium, p. 135.

Huffman, L. M. 1996. Processing of Whey protein for use as a food ingredient. Food Technol. 2:49-53

Jayaprakasha, H. M., R. S. Patel and E. Renner. 1994. Permeation behaviour of buffalo milk cheddar cheese whey during ultrafiltration Zeitschrift Lebensm, Untersuchung Forsch. 198(3):234-238.

Jayaprakasha, H. M., R. S. Patel, Y. C. Yoon and E. Renner. 1995. Critical aspects in the adoption of ultrafiltration whey processing - A review. Kor. J. Food Sci. Ani. Resour. 15(1):99-106.

Jayaprakasha, H. M., R. S. Patel and E. Renner. 1996. Characterisation of flux and composition during ultrafiltration and diafiltration of cheddar cheese whey. Ind. J. Chem. Technol. 3:140-144.

Jayaprakasha, H. M. and H. Brueckner. 1999. Whey protein concentrate : A potential functional ingredient for food industry. J. Food Sci. Technol. 36:189-204.

Kessler, H. G., C. Gernedel and K. Nakanishi. 1982. The effect of low molecular weight milk constituents on the flux in ultrafiltration. Milchwissenschaft. 37(10):584-588.

Lawrence, A. J. 1963. The determination of lactose in milk products. J. Dairy Technol. 23:103.

Lee, Y. R. and Y. H. Hong. 2003. Electrophoretic Behaviors of $\alpha$-Lactalbumin and $\beta$-Lactoglobulin Mixtures Caused by Heat Treatment. Asian-Aust. J. Anim. Sci. 16(7):1041-1045.

Marshall, K. R. and W. J. Harper. 1988. Whey protein concentrates. IDF Bulletin No. 233, 21.

Melachouris, N. 1984. Critical aspects in development of whey protein concentrate. J. Dairy Sci. 67:2693-2702.

Morr, C. V. and E. A. Foegeding. 1990. Composition and functionality of commercial whey and milk protein concentrates and isolates: A status report. Food Technol. 44:110-112.

Morr, C. V. 1979. Review: Functionality of whey protein products. Newzeland J. Dairy Sci. Technol. 14:185-188.

Patocka, J. and P. Jelen. 1987. Calcium chelation and other pretreatments for flx improvements in ultrafiltration of cottage cheese whey. J. Food Sci. 52:1241-1245 crossref(new window)

Renner, E. and Abd El-Salam. 1991. Application of ultrafiltration in the dairy industry. Elsevier Applied Sci. London, New york. pp. 158-161.

Rowland, S. J. 1938. The determination of nitrogen distribution in milk. J. Dairy Res. 9:42.

Tratnik, L. and L. Krsev. 1991. Production of whey protein concentrate. Milchwissenchaft 46(2):91-97.

Vuillemard, J. C., S. Gauthier and P. Paquin. 1989. Milk proteins as food ingredients. Lait 69:323-328.