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Effect of Arsenic on Immunity, Oxidative Enzyme and Various Hematological Parameters in Cross Bred Calves
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 Title & Authors
Effect of Arsenic on Immunity, Oxidative Enzyme and Various Hematological Parameters in Cross Bred Calves
Mishra, C.S.; Mani, Veena; Kaur, Harjit;
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An experiment was conducted on crossbred male calves to study the effect of arsenic (As) on immunity status and certain hematological parameters. Ten crossbred male calves of 3-4 months of age were distributed into two equal groups. Group I was kept as control, whereas, group II was supplemented daily with 50 ppm As (as ) up to 90 days, in the diet. Calves of both groups were fed as per ICAR standards and their requirements were fulfilled by feeding concentrate mixture and green oats. All calves were kept under similar managemental conditions. Blood samples were collected at fortnightly intervals to estimate various haematological parameters and superoxide dismutase (SOD) enzyme activity. Serum Ig and serum glutamic pyruvate transaminase (SGPT) were also measured. Cell-mediated immune responses of the calves were monitored at 0, 45 and 90 of experimental feeding, through lymphocyte proliferation. No change in blood total leukocyte counts (TLC), differential leukocyte counts (DLC), packed cell volume (PCV), haemoglobin (Hb) and SGPT was observed with As supplementation. A decrease in SOD activity was noticed in group II calves. Stimulation index (SI) for lymphocyte proliferation decreased from 1.14 to 0.79 in group II calves during 90 days experimental feeding, whereas, there was no change in SI values in group I indicating significant decrease in immune response of As supplemented calves. Blood As concentration increased in group II calves with the decrease in immune response. Short term supplementation of As to growing calves suggested suppressive effects on cell-mediated immunity. However, long term experiments are required to demonstrate clearly the efects of this toxic metal in calves.
 Cited by
Ahmad, S. 1995. Oxidative stress from environmental pollutants. Archives Insect Biochem. Physiol. 29:135-157.

Anonymous 2003. Bihar ke do gaon arsenic ki chapet mein, Dainik Bhaskar, p. 6, 27th Feb., 2003.

AOAC. 1995. Official methods of analysis, Association of Official Analytical Chemists. 16th Ed. Virginia, USA.

Chaudhari, A. N., S. Basu, S. Chattopadhyay and G. S. Das. 1999. Effect of high Arsenic content in drinking water on rat brains. Indian J. Biochem. Biophy. 36:51-54.

Chauhan, R. S. and S. K. Manipal. 1994. Immuno-toxicity of pesticides in poultry. In: Advances in Veterinary Research and their impact on animal health and production (Ed. R. Verma, T. N. Jaiswal and D. Swaroop) IAAVR, Bareilly, pp. 101-107.

Cobo, J. M. and M. Casteineria. 1997. Oxidative stress, mitochondrial respiration and glycemic control: Clue from Chronic supplementation with $Cr^{++}$ and $As^{++}$ to male wister rats. Nutrition, 13:965-91.

Dipankar, D., S. Gautam, B. K. Mandal, T. R. Choudhury, C. R. Chanda, P. P. Choudhury, G. K. Basu and C. Dipankar. 1996. Arsenic in ground water in six districts of West Bengal, India. Environmental Geochemistry and Health, 18:5-15.

Indian Council of Agricultural Research. 1998. Nutrients requirements for livestock and Poultry, ICAR, New Delhi.

Jain, N. C. 1986. Scham's Veterinary Heamatology, 4th ed. Lea and Febiger, Philadelphea.

Larson, H. J. 1979. A whole blood method for measuring mitogeninducedtransformation of sheep lymphocytes. Res. Vet. Sci. 27:334-338.

Marklund, S. and S. Marklund. 1974. Involvement of superoxide dismutase anion radical in auto-oxidation of pyrogallol and convenient assay for superoxide dismutase. European J. Biochem. 42:461.

McEvan, A. D. and E. W. Fisher. 1970. A turbidity test for the estimation of immunoglobulin level in neonatal calf serum. Clinical Chemica. Acta 17:155.

Oser, B. L. 1979. Hawk's Physiological Chemistry, 14th ed.Tata McGraw Hill Publishing Co. Ltd., New Delhi. pp. 1054-1093.

Pal, A., N. R. Jana, T. K. Sau, M. Bandopadhyaya and T. Pal. 1996. Flourometric determination of arsenic in water samples. First National Workshop on development and use of environmental reference materials. Laboratory Analytical Techniques Series: LATS/10/1997-98. Central Pollution Control Board, NewDelhi.

Sataka, M., Y. Mido, M. S. Sethi, S. A. Iqbal, H. Yasuhisa and S. Taguchi. 1997. Environmental toxicology. Discovery Publishing House, New Delhi, India.

Schalm, O. W. 1965. Veterinary Hematology, Lea and Febiger, Philadelphea.

Scultz, H., L. Nagymejtenyi, L. Institoris, A. Papp and O. Siroki. 2002. A study of behavioral neuro-toxicological and immunotoxicological effect of sub-chronic arsenic treatment in rats. J. Toxicol. Environ. Health, 65:1181-93.

Sengupta, M. and B. Bishayi. 2002. Effect of lead and arsenic on murine macrophage response. Drug Chem. Toxicol. 25:459-472.

Singh, R. B., R. C. Saha and R. K. Misra. 1998. Annual Report, National Dairy Research Institute, Karnal India.

Singh, R. B., R. C. Saha and R. K. Misra. 2001. Arsenic content of faeces, urine and blood of cattle in the arsenic affected villages in Nadia district of West Bengal. Proc. Xth Animal Nutr. Conf. NDRI, Karnal, India.

Snedecor, G. W. and W. G. Cochran. 1980. Statistical Methods. 6th ed. Oxford and IBH publishing Co. New Delhi.

Swenson, M. J. and W. O. Reece. 1996. Duke's Physiology of Domestic Animals. 11th Ed., Panima Publishing Co., New Delhi, India.

Vodela, J. K., S. D. Lenz, J. A. Rendon, W. H. Mc Ethenney and B. W. Kemppcinem. 1997. Drinking water contaminants: Effect on reproductive performance, egg quality and embryo toxicity in broiler breeder. Poult. Sci. 76:1493-1500.

Yu, H. S., W. T. Liao, K. L. Chang, G. L. Yu and G. S. Chen. 2002. Arsenic induces tumor necrosis factor alpha release and tumor factor receptor signalling in T- helper cells apoptosis. J. Insect. Dermatol. 119:812-819.

Zaman, K., R. S. Mc Gill, J. E. Jhonson, S. Ahmed and R. S. Pardini. 1995. An insect model for assessing oxidation stress related to arsenic toxicity. Archives Insect Biochem. Physiol. 29:199-209.