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Effects of Dietary Combinations of Vitamin A, E and Methionine on Growth Performance, Meat Quality and Immunity in Commercial Broilers
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Effects of Dietary Combinations of Vitamin A, E and Methionine on Growth Performance, Meat Quality and Immunity in Commercial Broilers
Lohakare, J.D.; Choi, J.Y.; Kim, J.K.; Yong, J.S.; Shim, Y.H.; Hahn, T.-W.; Chae, B.J.;
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The experiment was conducted to study the effect of dietary combinations of vitamin A (VA), vitamin E (VE) and methionine (Met) on growth performance, meat quality and immunity in commercial broilers. Ross chicks (n=3,630) were allocated to five experimental treatments with three replicates per diet. The dietary treatments were: VA 8,000 IU, VE 10 IU (diet 1); VA 12,000 IU, VE 10 IU (diet 2); VA 8,000 IU, VE 100 IU (diet 3); VA 12,000 IU, VE 100 IU (diet 4) and; VA 12,000 IU, VE 100 IU/kg diet and 20% Met higher than other groups (diet 5). The Met content in diet 1 to diet 4 were as per the requirement suggested by NRC. Separate vitamin premixes were prepared for each treatment diet as per the requirement of study. The 35 d study revealed significantly (p<0.0001) higher weight gains in broilers fed diet 3 and diet 5, than in the rest of the groups during starter phase (0-3 weeks) only. The feed intake did not vary significantly at all phases of study, but feed efficiency was significantly (p<0.05) lower in diet 1 during starter and overall phase (4-5 weeks). The bone strength and bone composition, except bone calcium, remained unaffected due to experimental diets studied after 35 d of experimental feeding. The thio-barbituric acid reactive substances (TBARS) were significantly (p=0.0013) lower in the breast meat in group 5, followed by group 3, than in the rest of the groups. The immune studies conducted, antibody titers to sheep red blood cells, thickness index to phytohaemagglutinin-P, and heterophil: lymphocyte ratio, did not show any significant difference among treatments. It could be concluded that supplementation of VA, VE and Met at higher levels could be beneficial to broilers only during the starter phase.
Vitamin A;Vitamin E;Methionine;Broiler;Bone;Immunity;
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Abawi, F. G. and T. W. Sullivan. 1989. Interactions of vitamins A, $D_3$, E and K in the diet of broiler chicks. Poult. Sci. 68:1490-1498.

Aburto, A. and W. M. Britton. 1998. Effects of different levels of vitamins A and E on the utilization of cholecalciferol by broiler chickens. Poult. Sci. 77:570-577.

AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists. Arlington, Virginia.

Bhargava, K. K., R. P. Hanson and M. L. Sunde. 1970. Effects of methionine and valine on antibody production in chicks infected with Newcastle disease virus. J. Nutr. 100:241.

Blakely, S. R., G. V. Mitchell, M. Y. Jenkins, E. Grundel and P. Whittaker. 1991. Canthaxanthin and excess vitamin A alter alpha-tocoplerol, carotenoid and iron status in adult rats. J. Nutr. 121:1649.

Ching, S., D. C. Mahan, T. G. Wiseman and N. D. Fastinger. 2002. Evaluating the antioxidant status of weanling pigs fed vitamin A and E. J. Anim. Sci. 80:2396-2401.

Coehlo, M. 2000. Poultry, swine, dairy vitamin supplementation updated. Feedstuffs.July 3 p. 12.

Deyhim, F. and R. G. Teeter. 1993. Dietary vitamin and/or trace mineral premix effects on performance, humoral mediated immunity, and carcass composition of broilers during thermo neutral and high ambient temperature distress. J. Appl. Poult. Res. 2:347-355.

De Vore, V. R., G. L. Colnago, L. S. Jensen and B. E. Greene. 1983. Thiobarbituric acid values and glutathione peroxidase activity in meat from chickens fed a Se supplemented diet. J. Food Sci. 48:300-301.

Franchini, A., A. Meluzzi, S. Bertuzzi and G. Giordani. 1988. High doses of vitamin E in the broiler diets. Archiv fur Geflugelkunde. 52:12-16.

Frankel, T. L. 1995. Sulfate incorporation into organic bone matrix of the tibiotarsus of broiler chicks is reduced by excess dietary methionine. Poult. Sci. 74(3):510-516.

Friedman, A., A. Meidovsky, G. Leitner and D. Sklan. 1991. Decreased resistance and immune response to Escherichia coli infection in chicks with low or high intakes of vitamin A. J. Nutr. 121:395-400.

Friedman, A. and D. Sklan. 1997. Effects of retinoids on immune response in birds. World Poult. Sci. J. 53:186-195.

Gross, W. B. and H. S. Siegel. 1983. Evaluation of the heterophil:lymphocyte ratio as a measure of stress in chickens. Avain Dis. 27:972-979.

Guo, Y., Q. Tang, J. Yuan and Z. Jiang. 2001. Effects of supplementation with vitamin E on the performance and the tissue peroxidation of broiler chicks and the stability of thigh meat against oxidative deterioration. Anim. Feed. Sci. Tech. 89:165-17.

Hickling, D., W. Guenter and M. Jackson. 1990. The effect of dietary lysine and methionine on broiler chicken performance and breast meat yield. Can. J. Anim. Sci. 70:673-678.

Jacobsen, K., R. M. Engberg, J. O. Andersen, S. K. Jensen, C. Lauridsen, P. Sorensen, P. Henckel, G. Bertelsen, L. H. Skibsted and C. Jensen. 1995. Supplementation of broiler diets with all-rac-alpha- or a mixture of natural source RRRalpha, gamma-delta-tocopheryl acetate. 1. Effect on vitamin E status of broilers in vivo and at slaughter. Poult. Sci. 74:1984-1994.

Jianlin, S., J. H. Kersey, C. A. Fritts and P. W. Waldroup. 2004. An evaluation of the interaction of lysine and methionine in diets for growing broilers. Int. J. Poult. Sci. 3(1):51-60.

Klasing, K. C. and D. M. Barnes. 1988. Decreased amino acid requirement of growing chicks due to immunological stress. J. Nutr. 118:1158-1164.

Korver, D. and K. Klasing. 2003. Influence of nutrition on immune status of the bird. In: Proceedings of the 24th Technical Turkey Conference p. 43.

Lauridsen, C., S. Hojsgaard and M. T. Sorensen. 1999. Influence of dietary rapeseed oil, vitamin E and copper on the performance and the oxidative status of pigs. J. Anim. Sci. 77:906-916.

Lauridsen, C., M. S. Hedemann and S. K. Jensen. 2001. Hydrolysis of tocopheryl and retinyl esters by porcine carboxyl ester hydrolase is affected by either carboxylate moiety and bile acids. J. Nutr. Biochem. 12:219-224.

Leeson, S. and J. D. Summers. 2001. Nutrition of the Chicken. (4th Edition). University Books Publication, Guelph, Canada.

Leshchinsky, T. V. and K. C. Klasing. 2001. Relationship between the levels of dietary vitamin E and the immune response of broiler chickens. Poult. Sci. 80:1590-1599.

Lohakare, J. D., T. W. Hahn, Y. H. Shim, J. Y. Choi and B. J. Chae. 2004. Effects of feeding methods (feed vs. water) of vitamin E on growth performance and meat quality of broilers. Asian-Aust. J. Anim. Sci. 17:1260-1265.

Mitsumoto, M., S. Ozawa, T. Mitsuhashi and K. Koide. 1998. Effect of dietary vitamin supplementation for one week before slaughter on drip, color and lipid stability during display in Japanese black steer beef. Meat Sci. 49:165-174.

Murphy, T. P., K. E. Wright and W. J. Pudelkiewicz. 1981. An apparent rachitogenic effect of excessive vitamin E intake in the chick. Poult. Sci. 60:1873-1878.

National Research Council. 1994. Nutrient Requirements of Poultry, 9th Edition. National Academy Press, Washington, DC.

Norazlina, M., S. Ima-Nirwana and B. A. K. Khalid. 1999. Effects of palm vitamin E, vitamin D and calcium supplementation on bone metabolism in vitamin E deficient rats. Med. J. Islam. Acad. Sci. 12(4).

Praharaj, N. K., M. R. Reddy, A. K. Panda, S. V. Ramarao and R. P. Sharma. 2002. Genotype by dietary lysine interaction for growth and response to sheep red blood cells and Escherichia coli inoculation in commercial broiler chicks. Asian-Aust. J. Anim. Sci. 15(8):1170-1177.

Ramarao, S. V., N. K. Praharaj, R. V. Reddy and A. K. Panda. 2003. Interaction between genotype and dietary concentrations of methionine for immune function in commercial broilers. Br. Poult. Sci. 44(1):104-112.

Rice, D. A. and S. Kennedy. 1988. Vitamin E and free radical formation. In: (Ed. W. Haresign and D. J. A. Cole) Recent Advances in Animal Nutrition, pp. 39-57 (London, Butterworths).

Ross, A. C. 1992. Vitamin A status: relationship to immunity and the antibody response. Proc. Soc. Exp. Bio. Med. 200:303-320.

SAS Institute. 1985. SAS User's Guide: Basics. SAS Institute Inc, Cary NC.

Schutte, J. B. and M. Pack. 1995. Sulfur amino acid requirement of broiler chicks from fourteen to thirty-eight days of age. 1. Performance and carcass yield. Poult. Sci. 74(3):480-487.

Sinnhubber, R. O. and T. C. Yu. 1977. The 2-thiobarbituric acid reaction, an objective measure of the oxidative determination occurring in fats and oils. J. Jan. Soc. Fish Sci. 26:259-267

Sklan, D. and S. Donoghue. 1982. Vitamin E response to high dietary vitamin A in the chick. J. Nutr. 112:759-765.

Swain, B. K. and T. S. Johri. 2000. Effect of supplemental methionine, choline and their combinations on the performance and immune response of broilers. Br. Poult. Sci. 41(1):83-88.

Tsiagbe, V. K., M. E. Cook, A.E. Harper and M. L. Sunde. 1987. Enhanced immune responses in broiler chicks fed methioninesupplemented diets. Poultry Sci. 66(7):1147-1154.

Veltmann, J. R., Jr. L. S. Jensen and G. N. Rowland. 1985. Exacerbative effect of vitamin A on malabsorption syndrome in chicks. Avian Diseases. 29: 446-456.

Wallis, I. R. 1999. Dietary supplements of methionine increase breast meat yield and decrease abdominal fat in growing broiler chickens. Aust. J. Exp. Agric. 39:131-141.

Wolbach, S. B. and D. M. Hegsted. 1952. Vitamin A deficiency in the duck. Skeletal growth and central nervous system. Arch. Pathol. 54:548.

Xu, H., B. A. Watkins and M. F. Seifert. 1994. Vitamin E status can alter bone histo-morphometry. J. Bone Min. Res. 9(1):S249.