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Effect of Removal of Follicles through Repeated Transvaginal Follicle Aspiration on Subsequent Follicular Populations in Murrah Buffalo (Bubalus bubalis
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 Title & Authors
Effect of Removal of Follicles through Repeated Transvaginal Follicle Aspiration on Subsequent Follicular Populations in Murrah Buffalo (Bubalus bubalis
Akshey, Y.S.; Palta, P.; Manik, R.S.; Vivekananad, Vivekananad; Chauhan, M.S.;
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 Abstract
This study was conducted to investigate the effects of removal of all ovarian follicles through repeated transvaginal follicle aspiration (TVFA) on the subsequent follicular populations in buffaloes. This information is crucial for determining the optimum time interval between successive aspirations for recovering oocytes from live buffaloes through Transvaginal Oocyte Retrieval (TVOR). The oestrus of cycling buffaloes (n=5) were synchronized by a single PGF injection schedule. All the ovarian follicles were removed once every 7 days for 6 weeks through TVFA, starting from Day 7 of the oestrous cycle (Day 0 = day of oestrus). The number and size of individual ovarian follicles was recorded at Day 3 and Day 5 (Day 0 = day of TVFA) through transrectal ultrasonography. The follicles were classified on the basis of their diameter as small (3-5 mm), medium (6-9 mm) and large (10 mm). There was no difference in the number of small and medium follicles, and the number of total follicles between Day 3 and Day 5. However, the number of large follicles was significantly higher (p<0.05) at Day 5 than that at Day 3. There was a significant (p<0.05) decrease in the proportion of small follicles and an increase (p<0.05) in the proportion of large follicles from Day 3 to Day 5, with no change in the proportion of medium follicles. The number of total follicles at Day 3 or Day 5 did not differ during the 6 TVFA sessions. It can be concluded that an interval of 3 days is more suitable than that of 5 days between successive aspirations for recovering oocytes through TVOR in a twice weekly schedule and that repeated removal of follicles through TVFA does not adversely affect the number of total follicles 3 or 5 days after TVFA.
 Keywords
Buffalo;Follicles;Ovum Pick-up;TVOR;
 Language
English
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 References
1.
Boni, R., S. Roviello and L. Zicarelli. 1996. Repeated ovum pick up in Italian Mediterranean buffalo cows. Theriogenology 46:899-909.

2.
Boni, R., M. W. M. Roelofsen, M. C. Pieterse, J. Kogut and Th. A. M. Kruip. 1997. Follicular dynamics, repeatability and predictability of follicular recruitment in cows undergoing repeated follicular puncture. Theriogenology 48:277-289.

3.
Broadbent, P. J., D. F. Dolman, R. G. Watt, A. K. Smith and M. F. Franklin. 1997. Effect of frequency of follicle aspiration on the oocyte yield and subsequent superovulatory response in cattle. Theriogenology 47:1027-1040.

4.
Dominguez, M. M. 1995. Effect of body condition, reproductive status and breed on follicular populations and oocyte quality in cows. Theriogenology 43:1405-1418.

5.
Garcia, A. and M. Salaheddine. 1998. Effect of repeated ultrasound-guided transvaginal follicular aspiration on bovine oocyte recovery and subsequent follicular development. Theriogenology 55:575-585.

6.
Gibbons, J. R., W. E. Beal, R. L. Krisher, E. G. Faber, R. E. Pearson and F. C. Gwazdauskas. 1994. Effects of once versus twice weekly transvaginal follicular aspiration on bovine oocyte recovery and embryo development. Theriogenology 42:405-419.

7.
Goodhand, K. L., R. G. Watt, M. E. Staines, J. S. M. Hutchinson and P. J. Broadbent. 1999. In vivo oocyte recovery and in vitro embryo production from bovine donors aspirated at different frequencies or following FSH treatment. Theriogenology 51:951-961.

8.
Hasler, J. F., W. B. Henderson, P. J. Hurtgen, Z. Q. Jin, A. D. Mccauley, S. A. Mower, B. Neely, L. S. Shuey, J. E. Stokes, and S. A. Trimmer. 1995. Production freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology 43:141-152.

9.
Kumar, A., V. S. Solanki, S. K. Jindal, V. N. Tripathi and G. C. Jain. 1997. Oocyte retrieval and histological studies of follicular population in buffalo ovaries. Anim. Reprod. Sci. 47:189-195.

10.
Le Van Ty, D. Chupin and M. A. Draincourt. 1989. Ovarian follicular populations in buffaloes and cows. Anim. Reprod. Sci. 19:171-178.

11.
Looney, C. R., B. R. Lindsey, C. J. Gonseth and D. L. Johnson. 1994. Commercial aspects of oocyte retrieval and in vitro fertilization (IVF) for embryo production in problem cows. Theriogenology 41:67-72.

12.
Mackey, D. R., J. M. Sreenan, J. F. Roche and M. G. Diskin. 1999. Effect of acute restriction on incidence of anovulation and periovulatory estradiol and gonadotropin concentrations in beef heifers. Biol. Reprod. 61:601-1607.

13.
Madan, M. L., S. K. Das and P. Palta. 1996. Application of reproductive technology to buffalo. Anim. Reprod. Sci. 42:299-306.

14.
Manik, R. S., J. D. Ambrose, S. K. Singla, M. S. Chauhan and M. L. Madan. 1994. Real time ultrasound evaluation of follicular changes in superovulated Murrah buffaloes. Buffalo J. 10:139-146.

15.
Manik, R. S., M. S. Chauhan, S. K. Singla and P. Palta. 2002a. Transvaginal ultrasound- guided aspiration of follicles from Indian buffaloes with reproductive problems. Vet. Rec.150:22-24.

16.
Manik, R. S., P. Palta, S. K. Singla and V. Sharma. 2002b. Folliculogenesis in buffalo (Bubalus bubalis): A review. Reprod. Fertil. Develop. 14:315-325.

17.
Meintjes, M., M. S. Bellow, J. R. Broussard, J. B. Paul and R. A. Godke. 1995. Transvaginal aspiration of oocytes from hormone treated pregnant beef cattle for in vitro fertilization. J. Anim. Sci. 73:967-974.

18.
Misra, A. K. 1997. Application of biotechnologies to buffalo breeding in India. Bubalus bubalis IV/97 (Suppl.), 141-166.

19.
Misra, A. K., B. V. Joshi, P. I. Agrawala, R. Kasiraj, S. Sivaiah, N. S. Rangareddi and M. U. Siddiqui. 1990. Multiple ovulation and embryo transfer in Indian buffalo (Bubalus bubalis). Theriogenology 33:1131-1141.

20.
Neglia, G., B. Gasparrini, V. C. di Brienza, R. Di Palo, G. Campanile, G. A. Presicce and L. Zicarelli. 2003. Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. Theriogenology 59:1123-1130.

21.
Palta, P. and M. S. Chauhan. 1998. Laboratory production of buffalo (Bubalus bubalis) embryos. Reprod. Fertil. Develop. 10:379-391.

22.
Revel, F., P. Mermillod, N. Peyhot, J. P. Rehard and Y. Heyman. 1995. Low developmental capacity of in vitro matured and fertilized oocytes from calves compared with that of cows. J. Reprod. Fert. 103:115-120.

23.
Singla, S. K., R. S. Manik and M. L. Madan. 1996. Embryo biotechnologies in buffaloes: A review. Bubalus bubalis I/96: 53-63.

24.
Taneja, M., P. E. T. Bols, A. V. de Velde, Tu Tyh-Cherng, D. Schreiber, M. W. Tripp, H. Levine, Z. Echelard, J. Piesen and X. Yang. 2000. Development competence of juvenile calf oocytes in vitro and in vivo: influence of donor animal variation and repeated gonadotropin stimulation. Biol. Reprod. 62:206-213.

25.
Techakumphu, M., A. Promdireg, A. Na-Chiengmai and N. Phutikanit. 2004. Repeated oocyte pick up in prepubertal Swamp buffalo (Bubalus bubalis) calves after FSH superstimulation. Theriogenology 61:1705-1711.