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Effects of Tween 80 and Fibrolytic Enzymes on Ruminal Fermentation and Digestibility of Feeds in Holstein Cows
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 Title & Authors
Effects of Tween 80 and Fibrolytic Enzymes on Ruminal Fermentation and Digestibility of Feeds in Holstein Cows
Baah, J.; Shelford, J.A.; Hristov, A.N.; McAllister, T.A.; Cheng, K.J.;
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The effects of the nonionic surfactant Tween 80 and a mixture of fibrolytic enzymes on total tract digestion, in situ disappearance (ISD) and ruminal fermentation characteristics of orchardgrass hay and barley grain were investigated in a 44 Latin square experiment with 4 non-lactating Holstein cows and 4 diets in 4 periods. Cows were offered a total mixed ration of 50% rolled barley grain and 50% orchardgrass hay treated with either 1) water (control), 2) 0.2% (vol/wt) Tween 80, 3) 0.2% (vol/wt) hydrolytic enzyme, or 4) 0.2% hydrolytic enzyme plus 0.2% Tween 80. Total tract digestibility coefficients of DM, nitrogen, NDF and ADF were not affected (p>0.05) by dietary treatment. Compared to the control, the rate of ISD of DM from orchardgrass hay was faster (p<0.05) in cows receiving diets treated with the enzyme alone or with enzyme plus Tween 80 (0.06/h vs. 0.076 and 0.069/h). The rate of digestion was lower (p<0.05) as compared to control when barley grain was treated with these additives. Ruminal fluid pH and concentrations of total VFA, acetate, isobutyrate and butyrate were not affected (p>0.05) by treatments. Cows that consumed diets treated with enzyme plus Tween 80 had higher (p<0.05) ruminal concentrations of propionate and isovalerate, and lower (p<0.05) acetate:propionate ratios. Compared to the control, microbial protein synthesis tended (p = 0.13) to increase with the addition of enzyme to the diet while nonammonia nitrogen flow to the duodenum increased (p<0.05) with both enzyme and Tween 80 treatments. The study indicated that fibrolytic enzymes alone or in combination with Tween 80 could enhance ISD of orchardgrass hay and ruminal concentrations of propionate, valerate and iso-valerate, but do not affect total tract digestibility.
Tween 80;Fibrolytic Enzyme;Digestibility;Dairy Cow;
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AOAC. 1990. Official Methods of Analysis. 15th edn. Association of Official Analytical Chemists, Washington, DC.

Akin, D. E. 1980. Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell walls. Appl. Environ. Microbiol. 39:242-252.

Beauchemin, K. A., L. M. Rode and V. J. H. Sewalt. 1995. Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forages. Can. J. Anim. Sci. 75:641-644.

Broderick, G. A. and J. H. Kang. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci. 63:64-75.

Canadian Council on Animal Care. 1993. Guide to the care and use of experimental animals. Vol. 1. (Ed. E. D. Olfert, B. M. Cross and A. A. McWilliam). CCAC, Ottawa, ON.

Classen, H. L. and M. R Bedford. 1991. The use of enzymes to improve the nutritive value of poultry feeds. In Recent Advances in Animal Nutrition. (Ed. P. Gimsworthy, W. Haresign and D. J. A. Cole). Butterworth–Heinemann, Boston, MA, USA. pp. 71-79.

Feng, P., C. W. Hunt, G. T. Pritchard and W. E. Julien. 1996. Effect of enzyme preparations on in situ and in vitro and in vivo digestive characteristics of mature cool-season grass forage in beef steers. J. Anim. Sci. 74:1349-1357.

Goto, M., H.-D. Bae, M. S. Yahaya, S. Karita, K. Wanjae, J. Baah, K. Sugawara and K. J. Cheng. 2003a. Effects of surfactant Tween 80 on enzymatic accessibility and degradation of orchardgrass (Dactylis glomerata L.) at different growth stages. Asian-Aust. J. Anim. Sci. 16:83-87.

Goto, M., H. Bae, S. S. Lee, M. S. Yahaya, S. Karita, K. Wanjae and K. J. Cheng. 2003b. Effects of surfactant Tween 80 on forage degradability and microbial growth on the in vitro rumen mixed and pure cultures. Asian-Aust. J. Anim. Sci. 16:672-676.

Hristov, A. N., T. A. McAllister and K.-J. Cheng. 1998. Effect of dietary or abomasal supplementation of exogenous polysaccharide-degrading enzymes on rumen fermentation and nutrient digestibility. J. Anim. Sci. 76:3146-3156.

Kamande, G. M. 1994. Manipulation of Rumen and Silage Fermentation. Ph.D. Thesis. University of British Columbia, Vancouver, BC.

Kamande, G. M., J. Baah, T. A. McAllister, K.-J. Cheng and J. A. Shelford. 2000. Effects of Tween 60 and Tween 80 on protease activity, thiol group reactivity, protein adsorption, and cellulose degradation by rumen microbial enzymes. J. Dairy Sci. 83:536-542.

Kim, C. H., J. N. Kim, J. K. Ha, S. G. Yun and S. S. Lee. 2004. Effects of dietary addition of surfactant Tween 80 on ruminal fermentation and nutrient digestibility of Hanwoo steers. Asian-Aust. J. Anim. Sci. 17:337-342.

Lee, S. S., B. H. Ahn, H. S. Kim, C. H. Kim, K.-J. Cheng and J. K. Ha. 2003. Effects of non-ionic surfactants on enzyme distributions of rumen contents, anaerobic growth of rumen microbes, rumen fermentation characteristics and performance of lactating cows. Asian-Aust. J. Anim. Sci. 16:104-115.

Lee, S. S. and J. K. Ha. 2003. Influences of surfactant Tween 80 on the gas production, cellulose digestion and enzyme activities by mixed rumen microorganisms. Asian-Aust. J. Anim. Sci. 16:1151-1157.

Lewis, G. E., W. K. Sanchez, C. W. Hunt, M. A. Guy, G. T. Pritchard, B. I. Swanson and R. J. Treacher. 1999. Effect of direct-fed fibrolytic enzymes on the lactational performance of dairy cows. J. Dairy Sci. 82:611-617.

McAllister, T. A., K. Stanford, H. D. Bae, R. J. Treacher, A. N. Hristov, J. Baah, J. A. Shelford and K.-J. Cheng. 2000. Effect of a surfactant and exogenous enzymes on digestibility of feed and on growth performance and carcass traits of lambs. Can. J. Anim. Sci. 80:35-44.

McAllister, T. A., S. J. Oosting, J. D. Popp, Z. Mir, L. J. Yanke, A. N. Hristov, R. J. Treacher and K.-J. Cheng. 1999. Effect of exogenous enzymes on digestibility of barley silage and growth performance of feedlot cattle. Can. J. Anim. Sci. 79:353-360.

Orskov, E. R. and I. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. (Camb.) 92:499-503.

Pritchard, G., C. Hunt, A. Allen and R. Treacher. 1996. Effect of direct-fed fibrolytic enzymes on digestion and growth performance in beef cattle. J. Anim. Sci. 74(Suppl. 1):296 (Abstr).

SAS. 1990. Statistical Analysis System Institute, User’s guide: Statistics. Version 6 edition. SAS Institute, Inc., Cary, NC, USA.

Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.

Van Soest, P. J. 1982. Nutritional Ecology of the Ruminant. O. and B. Books, Corvalis, OR, USA.

Wang, Y., T. A. McAllister, J. Baah, R. Wilde, K. A. Beauchemin, L. M. Rode, J. A. Shelford, G. M. Kamande and K.-J. Cheng. 2003. Effects of Tween 80 on in vitro fermentation of silages and interactive effects of Tween 80, monensin and exogenous fibrolytic enzymes on growth performance by feedlot cattle. Asian-Aust. J. Anim. Sci. 16:968-978.