Advanced SearchSearch Tips
Cloning of Bovine Macrophage Colony-stimulating Factor
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Cloning of Bovine Macrophage Colony-stimulating Factor
Kim, Tae-Yung; Kim, Cheol-Ho; Lee, Sang-Gil; Kang, Chung-Boo;
  PDF(new window)
Macrophage colony-stimulating factor (M-CSF) is a growth factor required for growth and differentiation of mononuclear phagocyte lineage. Total and 16 poly (A) mRNA of bovine M-CSF were isolated from healthy bovine peripheral mononuclear cells stimulated by phobol 12-myristste 13-acetate (TPA). The more compatible cultured mononuclear cells were 510/ml for RNA isolation. TPA-activated mononuclear cells increased the level of M-CSF-mRNA more than concanavalin A (Con A) and lipopolysaccharide (LPS). The optimal analysis of reverse transcriptase-polymerase chain reaction (RT-PCR) for14 Macrophage colonystimulating factor (M-CSF) as a growth factor required for bovine M-CSF was denaturation at 94 for 1 minute, annealing at 57 for 1 minute, extension at 72 for 1 minute for 30 cycles. The size of cDNA of bovine M-CSF by RT-PCR was 774 base pairs. A 774 base pairs cDNA encoding bovine M-CSF was synthesized by reverse transcriptase polymerase chain reaction (RT-PCR). Ligated cDNA was transformed to competent cells and then plasmid isolation and digestion was performed. Molecular cloning and sequencing were performed for cDNA of bovine M-CSF. The size of cloned cDNA of bovine M-CSF was 774base pairs. The homology of base sequence and amino acid sequence was 88% and 86% compared with known human M-CSF, respectively. From a high degree of sequence similarity, the obtained cDNA of bovine M-CSF is thought be a specific gene of bovine M-CSF.
 Cited by
Andreani, C. L., D. W. Payne, J. N. Packman, C. E. Resnick, A. Hurwitz and E. Y. Adashi. 1991. Cytokine-mediated regulation of ovarian function. Tumor necrosis factor $\alpha$ inhibits gonadotropin-supported ovarian androgen. J Biol Chem. 266(11):6761-6766.

Antczak, D. F. and N. T. Gorman. 1989. Cellular interaction in immune responses. In: (Ed. N. T. Gorman and R. E. Halliwell). Veterinary Clinical Immunology W.B. Sauders Co. Philadelphia. 107-123.

Bartelmez, S. H. and E. R. Stanley. 1985. Synergism between hemopoitic growth factors (HCFs) detected by their effects on cells bearing receptors for a lineage specific HGF: Assay of hemopoietin-1. J. Cell Physiol. 122:370-378.

Bartocci, A., J. W. Pollard and E. R. Stanley. 1983. Regulation of colony-stimulating factor during pregnancy. J. Exp. Med. 164:207-212.

Chen, B. D. M., H. S. Lin and S. Hus. 1983. Tumor-promoting phorbol esters inhibits the binding of olony-stimulating factor (CSF-1) to murine peritoneal exudate macrophages. J. Cell Physiol. 116:207-212.

Colditz, L. G. and P. J. C. M. Mass. 1987. The inflammatory activity of activated complement in ovine and bovine mammary glands. Immunol. Cell Biol. 65:433-436.

Craven, N. 1986. Chemotactic factor for bovine neutrophils in relation to mastitis. Comp. Immunol. Microbiol. Infect Dis. 9:29-36.

Das, S. K. and E. R. Stanley. 1981. Human colony-stimulating factor radioimmunoassay: Resolution of three subslasses of human colony-stimulating factors. Blood. 58(3):630-641.

Das, S. K. and E. R. Stanley. 1982. Structure-function of a colony stimulating factor (CSF-1). J. Biol. Chem. 257(22):13679-13684.

Duhamel, G. E. 1987. Distribution of T and B lymphocytes in mammary dry secretions. Colostrum and blood of adult dairy cattle. Vet. Immuno. Immunopathol. 14:101-122.

Guilbert, L. J. and E. R. Stanley. 1980. Specific interactions of murine colony-stimulating factor with mononuclear phagocytic cell. J. Cell Biol. 85:153-159.

Hanamura, T., K. Motoyoshi, K. Yoshida, M. Saito, Y. Miura, T. Kawashima, M. Nishida and F. Takaku. 1988. Quantitation and identification of human monocytic colony-stimulating factor in human serum by enzyme-linked immunosobent assay. Blood 72(3):886-892

Horiguchi, J., M. K. Warren and D. Kufe. 1987. Expression of the macrophage-specific colony-stimulating factor in human monocytes treated with granulocyte- macrophage colonystimulating factor. Blood 69:1259-1261.

Horiguchi, J., M. L. Sherman, A. Sampson-Johannes and D. Kufe. 1988. CSG-1 and C-form gene expression in human carcinoma cell lines. Biolchem. Biophys. Res. Commun. 157(1):395-401.

Kang, C. B. and S. D. Kwak. 1994. Activity and application of 20$\alpha$-hydroxysteroid dehydrogenase in rat. II. Changes in activities of the splenic macrophages and ovarian histological findings. Kor. J. Vet. Res. 34(4):873-880.

Kawasaki, E. S., M. B. Iadner, A. M. Wang, V. Arsdell, M. K. Warren, M. Y. Coyne, V. L. Schweichart, M. T. Lee, K. J. Wilson, A. Boosman, E. R. Stanley, P. Palph and D. F. Mark. 1985. Molecular cloning of a complementary DNA encoding human macrophage- specific colony stimulating factor (CSF-1). Science (Wash DC). 230:291-296. crossref(new window)

Manchester, K. L. 1995. Value of A260/280 ratio for measurement of purity of nucleic acid. Biothchniques. 19:208-219.

Metcalf, D. 1986. The molecualr biology and functions of the granulocyte-molecualr biology and function of the granulocyte-macrophage colony-stimulating factors. Blood 67:257-263.

Morgan, C. J. and E. R. Stanley. 1984. Chemical cross-linking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface. Biolchem. Biophys. Res. Commun. 119:35041.

Motoyoshi, K., F. Takaku and Y. Miura. 1983. High serum colonystimulating activity of leukocytopenic patients after intravenous infusions of human urinary colony- stimulating factor. Blood 62:685-691.

Motoyoshi, K., F. Takaku, T. Maekawa, Y. Miura, K. Kimura, S. Furasawa, M. Hattori, T. Nomura, H. Mizoguchi, M. Ogawa, K. Kinugasa, T. Tomminaga, M. Shimoyama, K. Deura, K. Ohta, T. Taguchi, T. Masaoka and I. Kimura. 1986. Protective effect of partially purified human urinary colony-stimulating factor on granulocytopenia after antitumor chemotheraphy. Exp. Hematol. 14:1069-1075.

Newby, T. J. and J. Bourne. 1977. The nature of the local immune system of the bovine mammary gland. J. Immunol. 118:461-465.

Oshima, K., H. Watanabe, K. Yoshihara, T. Kojima, O. Dochi, N. Takenouchi, M. Fukushima and M. Komatsu. 2003. Gene expression of leukemia inhibitory factor (LIF) and macrophage colony stimulating factor (M-CSF) in bovine endometrium during early pregnancy. Theriogenology 15;60(7):1217-26.

Ralph, P., M. K. Waren, M. T. Lee, L. J. Csejtey, J. F. Weaver, H. E. Broxmeyer, D. E. Williams, E. R. Stanley and E. S. Kawasaki. 1980. Inducible production of human macrophage growth factor, CSF-1. Bood 68:633-639.

Rambaldi, A., C. Y. Diane and J. D. Griffin. 1987. Expression of the M-CSF (CSF-1) gene by human monocytes. Blood 69:1409-1413.

Rambaldi, A., N. Wakamiya, E. Vellenga, J. Horiguchi, Warren MKim, D. Kufe and J. D. Grifffin. 1988. Expression of the macrophage colony colony-stimulating factor and c-fms genes in human acute myeloblastic leuksmia cells. J. Clin. Invest. 81:1030-1035.

Rettenmier, C. W., R. Sacca, W. L. Furman, M. F. Roussel, J. T. Holf, A. W. Nienhuis, E. R. Stanley and C. J. Sherr. 1986. Expression of the human c-fms proto- oncogen product (colony-stimulating factor-1 receptor) on peripheral blood mononuclear cells and choriocarcinoma cell lines. J. Clin. Invest. 77:1740-1746.

Sariban, E., T. Mitchell and D. Kufe. 1985. Expression of the cfms proto-oncogene during human monocytic differentiation. Nature (Lond). 316:64-66.

Sariban, E., K. Imamura, L. Robert and D. Kufe. 1988. Transcriptional and post transcriptional regulation of tumor necrosis factor gene expression in human monocytes. J .Clin. Invest. 81:1506-1510.

Stanley, E. R. and P. M. Heard. 1977. Factors regulating macrophage production and growth. J. Biol. Chem. 252(12):4305-4312.

Stanley, E. R., L. J. Guilbert, R. J. Tushinski and S. H. Bartelmez. 1983. CSF-1 A mononuclear phagocytic lineage-specific hemopoietic growth factor. J. Cell Biolchem. 21:151-159.

Tushinski, R. J., I. T. Oliver, L. J. Guilbert, P. W. Tynan, J. R. Warner and E. R. Stanley. 1982. Survival of mononuclear phagocytes depends on lineage-specific growth factor that the differentiated cells selectively destroy. Cell 28:71-81.

Wilfinger, W. W. and R. Cortini. 1997. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 22:474-481.

Yoshihara, K., S. Inumaru, Y. Hirota and E. Momotani. 1998. Cloning and sequencing of cDNA encoding bovine macrophage colony-stimulating factor (bM-CSF) and expression of recombinant bM-CSF using baculovirus. Vet. Immunol Immunopathol. 12;63(4):381-91.

Yoshihara, K., K. Oshima, Y. Muneta, R. Kikuma, C. Yayota, T. Hirai, N. Satoh, S. Matsuura, Y. Kikyo, M. Satoh, C. Kubota, S. Inumaru, Y. Yokomizo and Y. Mori. 2003. Quantitation of bovine macrophage colony-stimulating factor in bovine serum by ELISA. Vet. Immunol. Immunopathol. 15;95(3-4):103-11.