Advanced SearchSearch Tips
Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens
Xu, R.F.; Li, K.; Chen, G.H.; Qiang, B.Y.Z.; Mo, D.L.; Fan, B.; Li, C.C.; Yu, M.; Zhu, M.J.; Xiong, T.A.; Liu, Bang;
  PDF(new window)
New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.
Chicken;B-G Gene;PCR-RFLP;SNP;Nonsynonymous Substitution;
 Cited by
Abplanalp, H., K. Sato, D. Napoolitano and J. Reid. 1992. Reproductive of inbred congenic Leghorns carrying different haplotypes for the major histocompatibility complex. Poult. Sci. 71:9-17.

Bacon, L. D. 1987. Influence of the major histocompatibility complex on disease resistance and productivity. Poult. Sci. 66:802-811.

Briles, W. E., W. H. Mcgibbon and M. R. Irwin. 1950. On multiple alleles effecting cellular antigens in the chicken. Genetics.35:633-652.

Briles, W. E., R. M. Goto, C. Auffray and M. M. Miller. 1993. A polymorphic system related to but genetically independent of the chicken major histocompatibility complex. Immunogenetics. 37:408-414.

Bodmer, W. F. 1972. Evolutionary significance of the HL-A system. Nature 237:139-145.

Cahaner, A., E. J. Smith, S. Swenson and S. J. Lamont. 1996. Association of individual gnomic heterozygosity, estimated by molecular fingerprinting, and of dam major histocompatibility complex with growth and egg production traits in layer chickens. Poult. Sci. 75:1463-1467.

Cheng, G. C., F. M. Huang, Q. X. Zhou, W. C. Bo and Z. X. Duan. 2000. Germplasm Characteristics of Chinese Native Fowl Breeds, 1st edn. Shanghai Scientific & Technical Publishers, Shanghai, China.

Chen, G. H., K. H. Wang, J. Y. Wang, C. Ding and N. Yang. 2004. Poultry genetic resources in China, organized by the Committee of Poultry Genetic Resources in China, 1st edn. Shanghai Scientific & Technical Publishers, Shanghai, China.

Chu, M. X., C. L. Ji and G. H. Chen. 2003. Association between PCR-RFLP of Melatonin Receptor 1a Gene and High Prolificacy in Small Tail Han Sheep, Asian-Aust. J. Anim. Sci. 16(12):1701-1704.

Hala, K., J. Plachy and J. Schulmannova. 1981. Role of the B-Gregion antigen in the humoral immune response to the B-Fregion antigen of chicken MHC. Immunogenetics. 14:393-401.

Hunkapiller, T. and L. Hood. 1989. Diversity of the immunoglobulin gene superfamily. Adv Immunol. 44:1-63.

Iglesias, G. M., L. A. Soria, R. M. Goto, A. M. Jar, M. C. Miquel, O. J. Lopez and M. M. Miller. 2003. Genotypic variability at the major histocompatibility complex (B and Rfp-Y) in Camperos broiler chickens. Anim. Genet. 34:88-95.

Jarvi, S. I., R. M. Goto, G. F. Gee, W. E. Briles and M. M. Miller. 1999. Identification, inheritance, and linkage of B-G-like and MHC class I genes in cranes. J. Hered. 90:152-159.

Juul-Madsen, H. R., R. Zoorob, C. Auffray, K. Skjodt and J. E. Hedemand. 1997. New chicken Rfp-Y haplotypes on the basis of MHC class II RFLP and MLC analyses. Immunogenetics. 45:345-352.

Lakshmanan, N., J. S. Gavora and S. J. Lamont. 1997. Major histocompatibility complex class II DNA polymorphisms in chicken strains selected for Marek’s disease resistance and egg production or egg production alone. Poult. Sci. 76:1517-1523.

Takahata, N. and M. Nei. 1990. Allelic genealogy under overdominant and frequency-dependent selection and polymorphism of major histocompatibility complex loci. Genetics. 124:967-978.

Koshy a, M. P., G. Namkoong a and J. H. Roberds. 1998. Genetic variance in the F2 generation of divergently selected parents. Theor. Appl. Genet. 97:990-993.

Miller, M. M., R. Goto, S. Young, J. Chirivella, D. Hawke and C. G. Miyada. 1991. Immunoglobulin variable-region-like domains of diverse sequence within the major histocompatibility complex of the chicken. Proc. Natl. Acad. Sci. USA. 88:4377-381.

Miller, M. M., R. Goto, R. Zoorob, C. Auffray and W. E. Briles. 1994. Regions of homology shared by Rfp-Y and major histocompatibility B complex genes. Immunogenetics. 39:71-73.

Miller, M. M., R. M. Goto, Jr R. L. Taylor, R. Zoorob, C. Auffray, R. W. Briles, W. E.Briles and S. E. Bloom. 1996. Assignment of Rfp-Y to the chicken major histocompatibility complex/NOR microchromosome and evidence for highfrequency recombination associated with the nucleolar organizer region. Proc. Natl. Acad. Sci. USA. 93:3958-3962.

Padma, B., P. Kumar, V. Choudhary, S. K. Dhara, A. Mishra, T. K. Bhattacharya B. Bhushan and A. Sharma. 2004. Nucleotide Sequencing and PCR-RFLP of Insulin-like Growth Factor Binding Protein-3 Gene in Riverine Buffalo (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 17 (7):910-913.

Pharr, G. T., J. B. Dodgson, H. D. Hunt and L. D. Bacon. 1998. Class II MHC cDNAs in 15I5 B-congenic chickens. Immunogenetics. 47:350-354.

Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York.

Schierman, L. W. and A. W. Nordskog. 1961. Relationship of blood type to histocompatibility in chickens. Sci. 134:1008-1009.

Schierman, L. W. and A. W. Nordskog. 1963. Influence of the B bloodgroup-histocompatibility locus in chickens on a graftversus-host reaction. Nature 197:511-512.

Schierman, L. W. and A. W. Nordskog. 1964. Immunogenetic studies with fowl: relationship of blood groups to transplantation immunity and tolerance. Ann. NY. Acad. Sci. 120:348-355.

Schierman, L. W. and R. A. McBride. 1967. Adjuvant activity of erythrocyte isoantigens. Sci. 156:658-659.