Advanced SearchSearch Tips
Effect of Low Ambient Temperature on the Concentration of Free Radicals Related to Ascites in Broiler Chickens
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effect of Low Ambient Temperature on the Concentration of Free Radicals Related to Ascites in Broiler Chickens
Han, Bo; Yoon, Soon-Seek; Han, Hong-Ryul; Qu, Wei-jie; Nigussie, Fikru;
  PDF(new window)
A flock of Arbor Acres chickens were reared in cages and provided with high energy pelleted feed. At 14 d of age, a total of 350 birds were separated into 3 groups randomly as follows: 100 birds were exposed to ambient temperature of 20 as a control group, 150 birds were exposed to lower ambient temperature of 11 to induce ascites (group I), and another group of 100 birds were exposed to lower ambient temperature of 11 and fed diet containing 1% L-arginine for ascitic prophylactic treatment (group II). Blood and tissue samples (lung and liver) were collected from chickens at 3, 4, 5, 6 and 7 wk of age subsequently, to analyze the concentration and activities of free radicals, mononaldehyde (MDA), superoxide dismutase (SOD), Nitric Oxide (NO) and Nitric oxide synthase (NOS). The results showed that the prevalence of ascites in the control, group I and group II was 3%, 9.33% and 3% respectively (p<0.01). The concentration of free radicals in the lungs of 3 wks old preascitic broilers in group I was significantly higher than in the corresponding control group (p<0.05). The concentrations of free radicals in lung and liver in the 7 wk period, and that of NO and SOD in the plasma were significantly lower in group I than in the control group (p<0.01). However, the accumulated MDA contents in group I were higher than in the control group and group II (p<0.05), respectively. In the same way, the activity of NOS in group II was higher than both group I and control group (p<0.01) during the 7 wk period. There was no significant difference between SOD activities of group II and the control group (p>0.05), and also insignificant difference between NOS in group I and the control group (p>0.05). The results of this study indicate that there was a significant decrease in the concentration of MDA in group II. On the other hand, the concentration of free radicals decreased and MDA concentration increased in group I during the 7 wk period. The reduction in concentration of MDA in group II, following arginine supplementation may be associated with the scavenging activity of NO.
Ascites in Broilers;Free Radicals;MDA;SOD;NO;NOS;L-arginine;
 Cited by
Effects of Endothelin A Receptor Antagonist BQ123 on Femoral Artery Pressure and Pulmonary Artery Pressure in Broiler Chickens,Wang, Huiyu;Qiao, Jian;Gao, Mingyu;Yang, Ying;Li, Kai;Wang, Jianlin;Tian, Yong;Xu, Tong;

Asian-Australasian Journal of Animal Sciences, 2007. vol.20. 10, pp.1503-1509 crossref(new window)
Effect of Thyroid Hormones on the Redox Balance of Broiler Chickens,Lin, H.;Decuypere, E.;Buyse, J.;

Asian-Australasian Journal of Animal Sciences, 2008. vol.21. 6, pp.794-800 crossref(new window)
Barnes, P. J. 1990. Reactive oxygen species and airway inflammation. Free Radical Biology and Medicine 9:235-243.

Bottje, W. G., B. Enkvetchakul, R. Moore and R. McNew. 1995a. Robert Moore: Effect of a-tocopherol on antioxidants, lipid peroxidation and the incidence of pulmonary hypertension syndrome (ascites) in broilers. Poult. Sci. 74:1356-1369.

Bottje, W. G. and R. F. Wideman. 1995b. Potential role of free radicals in the pathogenesis of pulmonary hypertension syndrome. Poult. Av. Biol. Rev. 6:211-231.

Cawthon, D., M. Iqbal, J. Brand, R. McNew and W. G. Bottje. 2004. Investigation of proton conductance in liver mitochondria of broilers with pulmonary hypertension syndrome. Poult. Sci. 83(2):259-265.

Dawson, Y. L., G. L. Gores and A. L. Nieminen. 1993. Mitochondria as a source of reactive oxygen species during reductive stress in rats hepatocytes. Am. J. Phy. 264:C961-967.

Dorota, M. K., H. Tomasz, P. Jacek and P. Janusz. 2003. The effect of brief food withdrawal on the level of free radicals and other parameters of oxidative status in the liver. Med. Sci. Monit. 9(3):BR131-135.

Fridovich, I. 1978. The biology of oxygen radicals. Sci. 201:875-880.

Green, L. C., D. A. Wagner and J. Glogowsh. 1987. Analysis of nitrate, nitrite and nitrate in biological fluids. Anal. Bioch. 126:131-138.

Han, B., S. S. Yoon, H. R. Han and X. L. Wang. 2004a. Effects of L-arginine on endothelium derived factors and cyclic nucleotides in broilers under low ambient temperature. Asian-Aust. J. Anim. Sci. 17(11):1570-1581.

Han, B., S. S. Yoon, J. L. Su, H. R. Han, M. Wang, W. J. Qu and D. B. Zhong. 2004b. Effects of selenium, copper and magnesium on antioxidant enzymes and lipid peroxidation in bovine fluorosis. Asian-Aust. J. Anim. Sci. 17(12):1695-1699.

Iqbal, M., D. Cawthon, R. F. Wideman and W. G. Bottje. 2001. Lung mitochondrial dysfunction in pulmonary hypertension syndrome. II. Oxidative stress and inability to improve function with repeated additions of adenosine diphosphate. Poult. Sci. 80(5):656-665.

Iqbal, M., D. Cawthon, K. Beers, R. F. Wideman and W. G. Bottje. 2002. Antioxidant enzyme activities and mitochondrial fatty acids in pulmonary hypertension syndrome (PHS) in broilers. Poult. Sci. 81(2):252-60.

Julian, R. J. 1993. Ascites in poultry. Avian Pathol. 22:449-454.

Julian, R. J. 2004. Production and growth related disorders and other metabolic diseases of poultry: a review. The Vet. J. 168(3):215-223.

Kehrer, J. P. 1993. Free radicals as mediators of tissue injury and disease. Critical Reviews in Toxicology 23(1):21-48.

Maxwell, M. H., G. W. Robertson and C. Farquharson. 1996. Evidence of ultracytochemical mitochodria-derived hydrogen peroxide activity in myocardial cells from broiler chickens with an ascitic syndrome. Res. Vet. Sci. 61(1):7-12.

Mirsalimi, S. M., R. J. Julian and E. J. Squires. 1993. Effect of hypobaric hypoxia on slow- and fast-growing chickens fed diets with high and low protein levels. Avain Disease 37:660-667.

Moreno, S. M. and A. Hernandez. 2003. Nitric oxide synthase expression in the endothelium of pulmonary arterioles in normal and pulmonary hypertensive chickens subjected to chronic hypobaric hypoxia. Avian Dis. 47(4):1291-1297. crossref(new window)

Nebot, C., M. Moutet, P. Huet, J. Z. Xu, J. C. Yadan and J. Chaudiére. 1993. Spectrophotometric assay of superoxide dismutase activity based on the activated autoxidation of a tetracyclic catechol. Anal. Biochem. 214:442-451.

Park, Y. and J. P. Kehre. 1991. Oxidative changes in hypoxicreoxygenatedrabbit heart: a consequence of hypoxia rather than reoxygenation. Free Radical Research Communication. 14:179-185.

Rengasamy, A. and R. A. Johns. 1993. Inhibition of nitric oxide synthase by a superoxide generating system. Journal of Pharmocology and Experimental Therapeutics 267:1024-1027.

Sala, R., E. Moriggi, G. Corvasce and D. Morelli. 1993. Protection by N-acetylcysteine against pulmonary endothelial cell damage induced by oxidant injury. Euro. Resp. J. 6(3):440-446

SAS. 1994. SAS/STAT User's Guide: SAS Institute Inc., Cary. North Carolina.

Seccombe, J. F., P. J. Pearson and H. V. Schaff. 1994. Oxygen radical-mediated vascular injury selectively inhibits receptordependent release of nitric oxide from canine coronary artery cells. J. Thoracic and Cardiovascular Surgery 107:505-509.

Shah, A. M. and P. A. MacCarthy. 2000. Paracrine and autocrine effects of nitric oxide on myocardial function. Pharmacology and Therapeutics 86(1):49-86.

Wang, W., N. Inoue, T. Nakayama, M. Ishii and T. Kato. 1995. An assay method for nitric oxide synthase in crude samples by determining product NADP. Anal. Bioch. 227:274-280.

Wideman, R. F., M. Ismail, Y. K. Kirby, W. G. Bottje, R. W. Moore and R. C. Vardeman. 1995. Furosemide reduces the incidence of pulmonary hypertension syndrome (ascites) in broilers exposed to cool environmental temperatures. Poult. Sci. 74:314-322.

Yagi, K. 1976. A simlpe fluorometric assay for lipoperoxide in blood plasma. Biochem. Med. 15:212-216.

Zhang, L. J., Q. K. Yang and J. L. Sun. 2000. Study on the free radicals of soybean leaves in feeded with 1, 7 races of cercospora sojina hara. Soy. Sci. 19(1):21-25.