Advanced SearchSearch Tips
Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala
Suprabha, P.; Anilkumar, K.; Aravindakshan, T.V.; Raghunandanan, K.V.;
  PDF(new window)
Random amplified polymorphic DNA (RAPD-PCR) analysis of 56 animals of four different genetic groups of dwarf cattle in Kerala was done as a single step analysis. Bandsharing (BS) values were calculated for animals of each group and between groups as an analytical tool to find out genetic variation among animals. The different factors affecting BS values were estimated using Harvey''s Least squares analysis. The effects of genetic group, Guanine-cytosine (GC) content of primer and gel on BS values were found significant. Bandsharing values of Kasargode-Highrange dwarf animals were significantly different from Vechur, Vatakara and their combinations. The Vechur, Vatakara and Vechur-Vatakara combinations were found to be more uniform (high BS value) compared with other combinations. The bandsharing value was lowest with primers of GC content 90% and highest with 80% GC content. The effect of gel on BS value points to the need of adjustments of gel factor for calculation of BS values.
Random Amplified Polymorphic DNA Marker;Bandsharing Value;Dwarf Cattle;
 Cited by
Genetic Variation and Differences within and between Populations of Cultured and Wild Bullhead (Pseudobagrus fulvidraco) Revealed by RAPD-PCR,Yoon Jong-Man;Kim Gye-Woong;Park Hong-Yang;

Reproductive and Developmental Biology, 2005. vol.29. 4, pp.213-221
Ambady, S., C. M. Carpio and F. A. Ponce-de-Leon. 1996. Optimization of RAPD-PCR conditions in cattle. Anim. Biotech. 7(2):99-112

Aravindakshan, T. V. and A. M. Nainar. 1998. Genetic variation in cattle and buffalo breeds analysed by RAPD markers. Indian J. Dairy Sci. 51(6):368-374.

Bowditch, B. M., D. G. Albright, J. G. K. Williams and M. J. Braun. 1993. Use of randomly amplified polymorphic DNA markers in comparative genome analysis. Methods Enzymol. 224:294-309.

Dunnington, E. A., O. Gal, P. B. Siegel, A. Haberfeld, A. Cahaner, U. Lavi, Y. Plotsky and J. Hillel. 1991. Deoxyribonucleic acid fingerprint comparisons between selected populations of chicken. Poult. Sci. 70(3):463-467.

Gwakisa, P. S., S. J. Kemp and A. J. Teale. 1994. Characterization of zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. Anim. Genet. 25:89-94.

Islam, M. S., A. S. I. Ahemed, M. S. Azam and M. S. Alam. 2005. Genetic analysis of three river populations of Catla Catla (HAMILTON) using Randomly amplified Polymorphic DNA markers. Asian-Aust. J. Anim. Sci. 18(4):453-457.

Harvey, W. R. 1986. Mixed model Least-Squares and Maximum Likelihood Computer Programme, PC version (PC-1) LSMLMW with Parmcard.

Mohd-Azmi, M. L., A. S. Ali and W. K. Kheng. 2000. DNA fingerprinting of red jungle fowl, village chicken and broilers. Asian-Aust. J. Anim. Sci. 13(8):1040-1043.

Parejo, J. C., M. E. Sansinforiano, A. Rabasco, M. Martinez-Trancon, J. L. Fernandez-Garcia and J. A. Padilla. 1997. Optimization of the random amplified polymorphic DNA technique in white Caceres cattle. Archivos-de Zootecnia 46:279-289.

Penner, G. A., A. Bush, R. Wise, W. Kim, L. Domier, K. Kasha, A. Laroche, G. Scoles, S. J. Molnar and G. Fedak. 1993. Reproducibility of Random Amplified Polymorphic DNA (RAPD) analysis among Laboratories. PCR Methods and Applications 2:341-345.

Ramesha, K. P., T. Saravanan, M. K. Rao, M. M. Appannavar and A. O. Reddy. 2002. Genetic distance among South Indian breeds of zebu cattle using random amplified DNA markers. Asian-Aust. J. Anim. Sci. 15(3):309-314.

Rothuizen, J. and M. V. Wolferen. 1994. Randomly amplified DNA polymorphisms in dogs are reproducible and display Mendelian transmission. Anim. Genet. 25:13-18.

Saifi, H. W., B. Bharat, S. Kumar, P. Kumar, B. N. Patra and S. Arjava. 2004. Genetic identity between Bhadawari and Murrah breeds of Indian buffaloes (Bubalus bubalis) using RAPDPCR. Asian-Aust. J. Anim. Sci. 17(5):603-607.

Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22):6531-6535.

Yoon, J. and Kim Jong-Yeon. 2004. Genetic differences within and between populations of Korean cat fish (S. astous) and Bull head (P. fulvidraco) analysed by RAPD-PCR. Asian-Aust. J. Anim. Sci. 17(8):1053-1061.

Yoon, J. and H. Park. 2002. Genetic similarity and variation in the cultured and wild crucian carp (Carassius carassius) estimated with random amplified polymorphic DNA. Asian-Aust. J. Anim. Sci. 15(4):470-476.