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Characterization of MHC DRB3.2 Alleles of Crossbred Cattle by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
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Characterization of MHC DRB3.2 Alleles of Crossbred Cattle by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
Paswan, Chandan; Bhushan, Bharat; Patra, B.N.; Kumar, Pushpendra; Sharma, Arjava; Dandapat, S.; Tomar, A.K.S.; Dutt, Triveni;
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The present investigation was undertaken to study the genetic polymorphism of the DRB3 exon 2 in 75 crossbred cattle by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Five genotypes i.e. HaeIII-a, HaeIII-b, HaeIII-e, HaeIII-ab and HaeIII-ae were observed when the 284 bp PCR products were digested with HaeIII restriction enzyme. The corresponding frequencies of these patterns were 0.53, 0.04, 0.01, 0.38 and 0.04, respectively. Digestion with RsaI restriction enzyme resolved 24 different restriction patterns. The frequencies of these patterns ranged from 0.013 (RsaI-f, RsaI-k and RsaI-c/n) to 0.120 (RsaI-n). The results revealed that the crossbred cows belonged to the RsaI patterns namely b, k, l, a/l, d/s, l/n, l/o and m/n, whose corresponding frequencies were 0.027, 0.013, 0.040, 0.027, 0.040, 0.067, 0.027 and 0.067, respectively. Digestion of the 284 bp PCR product of DRB3.2 gene with PstI in the crossbred cattle did not reveal any restriction site. These results suggested the absence of the recognition site in some of the animals. These results also revealed that the crossbred cows studied were in homozygous as well as heterozygous condition. On the basis of the above results it can be concluded that the DRB3.2 gene was found to be highly polymorphic in the crossbred cattle population.
DRB3.2 Gene;PCR-RFLP;Crossbred Cattle;
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아세아태평양축산학회지, 2007. vol.20. 12, pp.1791-1797 crossref(new window)
Aldridge, B. M., S. M. McGuirk, R. J. Clark, L. A. Knapp, D. I. Watkins and D. P. Lunn. 1998. Denaturing gradient gel electrophoresis a rapid method for differentiating BoLA-DRB3 alleles. Anim. Genet. 29:389-394.

Allen, P. M., B. P. Babbitt and E. R. Unanue. 1987. T-cell recognition of lysozyme: the biochemical basis of presentation. Immuno. Rev. 98:171-187.

Amorena, B. and W. H. Stone. 1978. Serologically defined (SD) locus in cattle. Sci. 201:159-160.

Andersson, L. and C. J. Davies. 1994. The major histocompatibility complex. In: Cell-Mediated Immunity in Ruminants (Ed. B. M. L. Goddeeris and W. L. Morrison), pp. 37-57. CRC press, Boca Raton, FL.

Andersson, L., J. Bohme, L. Rask and P. A. Peterson. 1986. Genomic hybridization of bovine class II Major histocompatibility gene, extensive polymorphism of DQa and DQb genes. Anim. Genet. 17:95-112.

Aravindakshan, T. V. and A. M. Nainar. 1999. Genetic polymorphism of BoLA DRB3 gene in Jersey crossbred and Ongole cattle revealed by PCR-RFLP. Indian J. Anim. Sci. 69:424-427.

Bernoco, D., H. A. Lewin, L. Andersson, M. A. Arriens, G. Byrns, S. Cwik, C. J. Davies, H. C. Hines, W. Leibold, O. Lie, D. Meggiolaro, R. A. Oliver, H. Ostergard, R. L. Spooner, J. A. Stewart-Haynes, A. J. Teale, J. W. Templeton and M. Zanotti. 1992. Joint report of the Fourth International Bovine Lymphocyte Antigen (BoLA) Workshop, East Lansing, Michigan, USA, 25 August 1990. (Cited: Anim. Genet. (1992) 22:477-496).

Biozzi, G., C. Stiffel, D. Mouton and Y. Bouthiller. 1974. Selection of mice with high and low antibody response to complex immunogens. In: Immunogenetics and Immunodeficiency (Ed. B. Bennacerraf), pp. 180-225. University Park Press, Baltimore.

Davies, C. J., L. Andersson, S. A. Ellis, J. E. Hensen, H. A. Lewin, S. Mikko, N. E. Muggli-Cocket, J. J. Poel van der and G. C. Russel. 1997. Nomenclature for factors of the BoLA system, 1996. Report of the ISAG BoLA Nomenclature committee. Anim. Genet. 28:159-168.

Dechamma, H. J., C. Natarajan, V. V. S. Suryanarayanan and T. J. Rasool. 1998. Nucleotide sequence of DRB-exon of MHC from an Indian zebu cattle breed. Indian J. Exptl. Biol. 36:879-886.

De, Sachinandan. 2000. Molecular characterization of DRB exon 2 in cattle and buffalo. Ph.D. Thesis submitted to Deemed University, I.V.R.I., Izatnagar.

Emery, D. L., N. K. Puri, J. H. Dufty, M. D. Gorrell and M. R. Brandon. 1987. A functional and biochemical analysis of bovine class II MHC antigens using monoclonal antibodies. Vet. Immuno. and Immunopath. 16:215-234.

Gelhaus, A., L. Schnittger, D. Mehlitz, R. D. Horstmann and C. G. Meyer. 1995. Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles. Anim. Genet. 26:147-153.

Gilliespie, B. E., B. M. Jayrao, H. H. Dowlen and S. P. Oliver. 1999. Analysis and frequency of BoLA-DRB3.2 alleles in Jersey cows. J. Dairy Sci. 82:2049-2053.

Glass, E. J., R. A. Oliver, J. L. Williams and P. Millar. 1992. Alloreactive T-cell recognition of bovine major histocompatibility complex class II products defined by onedimensional isoelectric focusing. Anim. Genet. 23:97-111.

Groenen, M., J. Van Der Poel, R. Dijkhof and M. Giphart. 1990. The nucleotide sequence of bovine MHC class II DQB and DRB genes. Immunogenetics, 31:37-44.

Klein, J. 1986. Natural History of the Major Histocompatibility Complex. John Wiley and Son, New York.

Maillard, J. C., C. Renard, P. Chardon, I. Chantal and A. Bensaid. 1999. Characterization of 18 new BoLA- DRB3 alleles. Anim. Genet. 30:200-203.

Mallard, B.A., B. N. Wilkie and B. W. Kennedy. 1989. Genetic and other effects on antibody and cell mediated immune response in swine leukocyte antigen (SLA) - defined miniature pigs. Anim. Genet. 20:167-178.

Poli, M., L. Sala and R. Zandomeni. 2003. Identification of a new BoLA-DRB3.2 allele. Anim. Genet. 34:384-397.

Sharma, A. K., B. Bhushan, S. Kumar, Pushpendra Kumar and Arjava Sharma. 2004. Molecular characterization of Rathi and Tharparkar indigenous cattle (Bos indicus) breeds by RAPD-PCR. Asian-Aust. J. Anim. Sci. 17:1204-1209.

Singh, S . 2001. Molecular genetic characterization of DRB 3.2 exon polymorphism in Murrah buffalo. M. V. Sc Thesis submitted to Deemed University, I.V.R.I., Izatnagar., p.43.

Sitte K., I. J. East, M. F. Lavin and E. C. Jazwinska. 1995. Identification and characterization of new BoLA-DRB3 alleles by heteroduplex analysis and direct sequencing. Anim. Genet. 26:413-417.

Spooner, R. L., H. Leveziel, F. Groslaude, R. A. Olive and M. Vaiman. 1978. Evidence for a possible major histocompatibility complex (BLA) in cattle. Journal of Immunogenetics 5:335-346.

Takeshima, S., M. Ikegami, Y. Nakai, M. Morita and Y. Aida. 2000. Identification of BoLA- DRB3 in Japanese black cattle by PCR sequence-based typing. ISAG 2000 (abstract). Proceeding of the 27th International Conference of Animals Genetics, July 22-26, 2000. Minneapolis, Minnesota, p. 82.

Van Eijk, M. J. T., J. A. Stewart-Haynes and H. A. Lewin. 1992. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP. Anim. Genet. 23:483-489.

Wang, W, K. Ouyang, J. Ouyang, H. Li, S. Lin and H. Sun. 2004. Polymorphism of insulin-like growth factor I gene in six chicken breeds and its relationship with growth traits. Asian-Aust. J. Anim. Sci. 17:301-304.

Zhang, B, Z. M. G. Lei., C. Y. Deng, Y. H. Xiong, B. Zuo and F. E. Li. 2005. Association between PCR-RFLP polymorphism of the fifth intron in lipoprotein lipase gene and productive traits in pig resource family. Asian-Aust. J. Anim. Sci. 18:458-462.